Application of oligo-fructose, mannose and derivatives thereof in preparation of functional low-harm wines and anti-inebriation products
A technology of fructooligosaccharides and mannose, which is applied in the field of functional low-damage wine or hangover products, and the preparation of functional low-damage wine or hangover products
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Embodiment 1
[0032] Example 1: Accelerating the metabolism of ethanol and reducing the damage of drinking to the body
[0033] 1. Materials and methods
[0034] 1.1 Materials: Wistar rats were purchased from the Institute of Experimental Animals of the Third Military Medical University of the Chinese People's Liberation Army, with a body weight of 220±10 g, half male and half male, kept in separate cages, free to drink water, and fed with standard rat diet. Multifunctional microscope, BX410, Japan Olympus company; digital camera, A620, Japan company. Commercially available ordinary liquor with an alcohol content of 38°. Mannose, commercially available.
[0035] 1.2 Intervention methods of mannose on ethanol-induced liver injury
[0036] Rats were randomly divided into 3 groups (20 in each group):
[0037] Normal control group: intragastric administration of normal saline at a dose of 0.168ml / kg / d;
[0038] Model control group: each mouse was fed with 38° liquor, and the alcohol intake...
Embodiment 2
[0067] Example 2: Slow down the absorption of ethanol in the gastrointestinal tract experiment
[0068] 1. Materials and methods
[0069] 1.1 Materials: Wistar rats were purchased from the Institute of Experimental Animals of the Third Military Medical University of the Chinese People's Liberation Army, with a body weight of 220±10 g, half male and half male, kept in separate cages, free to drink water, and fed with standard rat diet. Multifunctional microscope, BX41, Japan Olympus company; digital camera, A620, Japan company. Commercially available Laobaigan with an alcohol content of 38°. N-acetylaminomannose, commercially available; prepared as a 0.5% ethanol solution for subsequent use.
[0070] 1.2 Method
[0071] Twenty healthy SD male rats were randomly divided into 2 groups:
[0072] Model control group: each mouse was fed with 38° liquor, and the alcohol intake was 0.168ml / kg / d;
[0073] N-acetylamino mannose intervention group: Each mouse was fed with 1‰ (0.1g p...
Embodiment 3
[0097] Example 3: Intervention of Alcohol Refined Intestinal Mucosa Barrier Damage to Reduce the Effect of Alcoholic Liver Disease
[0098] 1. Materials and methods
[0099] 1.1 Materials: fructo-oligosaccharide and mannose, commercially available; liquor, commercially available, alcohol content 38°, 65°, produced by Chongqing Shixian Taibai Group; KM mice, purchased from the Institute of Experimental Animals, Third Military Medical University of the Chinese People’s Liberation Army , body weight 20±2g, half male and half female, conventional feeding; multifunctional microscope, BX410, Japan Olympus Company; digital camera, A620, Canon digital camera.
[0100] 1.4 Intervention of fructooligosaccharides and mannose on ethanol-induced liver injury
[0101] Divide 30 mice equally into 3 groups:
[0102] Normal control group: intragastric administration of normal saline at a dose of 0.168ml / kg / d;
[0103] Model control group: each mouse was fed with 38° liquor, and the alcohol ...
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Abstract
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