Fermentation culture medium and fermentation method for producing pentostatin by fermentation of streptomyces antibioticus
A fermentation medium and anti-streptomyces technology, applied in the field of industrial microorganisms, can solve the problem of low fermentation units of pentostatin, and achieve the effect of increasing production and increasing fermentation units
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Embodiment 1
[0030] Streptomyces antibioticus NRRL3238 (purchased from the US Department of Agriculture Culture Collection) was streaked on the seed slant medium, cultured at 28°C for 7 days, and then inoculated into the seed medium with a loading volume of 20ml / 250ml. ℃, 250rpm rotary shaker for 36h. The obtained fermented seeds were inoculated in a 5L fermenter containing 2L medium with a 1% (v / v) inoculation amount, the fermentation temperature was automatically controlled to 30°C, pH 6.5, the speed was related to dissolved oxygen, and the dissolved oxygen was maintained at 40 %, ventilation volume 0.5VVM. At 24h, start to feed glucose 1-10‰L / h, and the fermentation period is 72h. The output of pentostatin reaches 50mg / L.
[0031] The slant medium used therein consists of: glucose 4.0g / L, yeast extract powder 4.0g / L, malt extract powder 10.0g / L, agar powder, 20.0g / L, pH natural.
[0032] The composition of the seed medium used: 2% glucose, 2% soybean cake powder, 0.5% NaCl, 0.25% CaC...
Embodiment 2
[0035] The 1% (v / v) inoculum of the seeds prepared in Example 1 was inoculated in the fermentation medium for fermentation. Fermentation medium composition: glucose 10.0g / L, fine bean dregs 30.0g / L, yeast extract powder 5.0g / L, NaCl 5.0g / L. Automatically control the fermentation temperature to 30°C, pH to 6.5, dissolved oxygen to 40%, ventilation to 0.5VVM, and start to feed glucose at 1 to 10‰L / h every 24 hours. The fermentation period was 72h, and the output of pentostatin reached 20mg / L.
Embodiment 3
[0037] The 1% (v / v) inoculum of the seeds prepared in Example 1 was inoculated in the fermentation medium for fermentation. Fermentation medium composition: glucose 60.0g / L, fine bean dregs 30.0g / L, yeast extract powder 5.0g / L, NaCl 5.0g / L. Automatically control the fermentation temperature to 30°C, pH to 6.5, dissolved oxygen to 40%, ventilation to 0.5VVM, and start to feed glucose at 1 to 10‰L / h every 24 hours. The fermentation period was 72h, and the output of pentostatin reached 30mg / L.
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