Semen diluter and preparation method as well as application method thereof

Abstract

The invention relates to a semen diluter and a preparation method as well as application method thereof. The semen diluter is mainly used for diluting and preserving the semen of breeder cocks. The semen diluter is prepared from the following components: 0.1-10g of glucose, 0.1-2g of amikacini sulfatis for injection, 0.1-2g of cefotaxime sodium for injection, 0.1-5ml of yolk liquid and 100ml of normal saline. The preparation method of the semen diluter comprises the following steps of: (1) respectively dissolving the glucose, the amikacini sulfatis for injection and the cefotaxime sodium for injection in the normal saline; and (2) adding the yolk liquid, and uniformly mixing to prepare the semen diluter. The ratio of the prepared semen diluter to the semen to be diluted is (1:1.5)-(1.5:1). After the semen is diluted by the diluter, the utilization ratio of the semen can be improved, the preservation time of the semen can be prolonged, the semen can be preserved in a wider temperature range, and higher motility and activity of the semen can be kept.

Description

technical field [0001] The invention relates to a semen diluent, which is used for diluting collected semen during artificial insemination, especially for artificial insemination of poultry such as chickens, etc. The invention also relates to a preparation method and using method of the semen diluent . Background technique [0002] In order to improve the semen utilization rate of the rooster, prolong the storage time, and not significantly reduce the vitality of the sperm, the method usually adopted in the prior art is: after the semen of the rooster is collected into the semen collection device, the temperature is similar to that of the original semen collection. The semen collection device is stored in a constant temperature water bath with warm water, usually the water temperature is kept at 36.5-37 ℃. [0003] The temperature requirements are stricter when using this method, and the viability of sperm after 30 minutes of storage is usually reduced from the initial 70-8...

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Problems solved by technology

[0003] When this method is used for storage, the temperature requirements are strict, and the sperm viability usually decreases from the initial 70-80% to 30-40% after 30 minutes of storage, which not only makes the semen not fully utilized, but also saves for a short time. The actual operation is more difficult, and it needs to be stored and used at the same time. In order to improve the fertilization rate, the time from collection to use should generally not exceed 30 minutes

[0004] For the semen that needs to be stored for a long time, the method of low temperature storage is generally used in the prior art, such as storage at 5-10°C, to prolong the storage time and facilitate transportation, etc., but the storage time is generally not more than 24 hours, which cannot meet the requirements. actual needs

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