Application of polydatin in the preparation of sunscreen cosmetics
A technology of cosmetics, polydatin, applied in the field of natural plant extract polydatin
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Embodiment 1
[0038] Embodiment 1, Ultraviolet (UVB) irradiation dose determination of HaCaT cells
[0039] HaCaT cells in 96-well plates, using 30mJ / cm 2 、60mJ / cm 2 、90mJ / cm 2 and 120mJ / cm 2 The UVB dose was irradiated, and a blank group that did not receive irradiation was set up. The cell viability of each group was measured by MTS method as follows: figure 1 . figure 1 It shows that the semi-lethal dose of UVB irradiated HaCaT cells is 90mJ / cm 2 , so all future experiments use 90mJ / cm 2 As the damaging dose of UVB irradiated cells.
Embodiment 2
[0040] Embodiment 2, the protective effect of polydatin on HaCaT cells irradiated by UVB
[0041] UVB irradiation made the lethality of HaCaT cells in the control group less than half lethal, and greatly reduced the proliferation activity of HaCaT cells. When the concentration of polydatin is 10 μg / ml, it can significantly protect HaCaT cells from UVB damage, such as figure 2 Shown, and this protective effect is dose-dependent in the concentration of polydatin in the range of 10-200 μg / ml. figure 2 Compared with the UV control group, *P<0.05, **P<0.01, ***P<0.001.
Embodiment 3
[0042] Embodiment 3, the effect of polydatin on the activity of HaCaT cells
[0043] After HaCaT cells were treated with different concentrations of polydatin (20 μg / ml, 40 μg / ml, 80 μg / ml, 100 μg / ml, 150 μg / ml, 200 μg / ml) for 24 hours, the cell proliferation activity was detected by MTS method, and the results were as follows: image 3 shown. image 3 Among them, compared with the UV control group, *P<0.05, **P<0.01, ***P<0.001. When the concentration of polydatin is below 80 μg / ml, it has almost no effect on the proliferation activity of HaCaT cells; while when the concentration of polydatin reaches 100 μg / ml and above, it can significantly inhibit the proliferation activity of HaCaT cells. Therefore, polydatin concentrations of 20 μg / ml, 40 μg / ml and 80 μg / ml were selected as drug concentrations for cell and molecular mechanism research in the present invention.
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