Preparation method of hyaluronic acid and chitosan composite polyelectrolyte nanofibers

A composite polyelectrolyte, composite nanofiber technology, applied in fiber processing, fiber chemical characteristics, textiles and papermaking, etc., can solve the problems of material properties and adverse effects of application, and achieve good biocompatibility and cell adhesion rate, The effect of increasing the specific surface area

Active Publication Date: 2011-12-14
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature reports (Electrospinning of chitosan solution in acetic acid with poly, Journal of Biomaterials Science: Polymer Edition 2004,15), adding polyoxyethylene to the 2% acetic acid solution of chitosan improved the performance of chitosan and obtained Chitosan/polyoxye

Method used

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  • Preparation method of hyaluronic acid and chitosan composite polyelectrolyte nanofibers
  • Preparation method of hyaluronic acid and chitosan composite polyelectrolyte nanofibers
  • Preparation method of hyaluronic acid and chitosan composite polyelectrolyte nanofibers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Prepare a mixed solvent of deionized water and formic acid with a mass ratio of 25:75, and hyaluronic acid (Mw= 1 000 000 g mol -1 ) directly dissolved in the solvent, stirred at room temperature until the solution was transparent to make a 1wt% hyaluronic acid solution;

[0029] 2) Chitosan (Mw= 200 000 g·mol -1 ) was dissolved in a mixed solvent of deionized water and formic acid with a mass ratio of 30:70 to prepare a chitosan solution with a concentration of 6wt%;

[0030] 3) Mix the chitosan solution in step 2 and the hyaluronic acid solution in step 1 at a volume ratio of 1:9, and stir for 3 hours to obtain a uniform electrospinning solution;

[0031] 4) Put the electrospinning solution in step 3 into a 5ml syringe, set the extrusion speed to 0.1ml / h; the spinning voltage is 20kV, the distance from the spinning nozzle to the receiver is 15cm, start spinning, and that’s it Obtain the natural polymer nanofibers with pH-adjustable chargeability.

[0032] After ...

Embodiment 2

[0035] 1) Prepare a mixed solvent of deionized water and formic acid with a mass ratio of 30:70, and hyaluronic acid (Mw= 1 000 000 g mol -1 ) directly dissolved in the solvent, stirred at room temperature until the solution was transparent to make a 1wt% hyaluronic acid solution;

[0036] 2) Chitosan (Mw= 200 000 g·mol -1 ) was dissolved in a mixed solvent of deionized water and formic acid with a mass ratio of 20:80 to prepare a chitosan solution with a concentration of 6wt%;

[0037] 3) Mix the chitosan solution in step 2 and the hyaluronic acid solution in step 1 at a volume ratio of 1:9, and stir for 3 hours to obtain a uniform electrospinning solution;

[0038] 4) Put the electrospinning solution in step 3 into a 5ml syringe, set the extrusion speed to 0.1ml / h; the spinning voltage is 20kV, the distance from the spinning nozzle to the receiver is 15cm, start spinning, and that’s it Obtain the natural polymer nanofibers with pH-adjustable chargeability.

[0039]

Embodiment 3

[0041] 1) Prepare a mixed solvent of deionized water and formic acid with a mass ratio of 50:50, and hyaluronic acid (Mw= 1 000 000 g mol -1 ) directly dissolved in the solvent, stirred at room temperature until the solution was transparent to make a 1wt% hyaluronic acid solution;

[0042]2) Dissolve chitosan (Mw=200 000 g mol-1) in a mixed solvent of deionized water and formic acid with a mass ratio of 10:90 to prepare a chitosan solution with a concentration of 6wt%;

[0043] 3) Mix the chitosan solution in step 2 and the hyaluronic acid solution in step 1 at a volume ratio of 1:9, and stir for 3 hours to obtain a uniform electrospinning solution;

[0044] 4) Put the electrospinning solution in step 3 into a 5ml syringe, set the extrusion speed to 0.1ml / h; the spinning voltage is 20kV, the distance from the spinning nozzle to the receiver is 15cm, start spinning, and that’s it Obtain the natural polymer nanofibers with pH-adjustable chargeability.

[0045]

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Abstract

The invention discloses a preparation method of hyaluronic acid and chitosan composite nanofibers. According to the preparation method, natural products, namely the chitosan and the hyaluronic acid are used as raw materials, and the composite polyelectrolyte nanofibers are prepared by preparing a soluble composite polyelectrolyte mixture solution from the chitosan and the hyaluronic acid and adopting a high-pressure electrostatic spinning technology. The nanofibers prepared by adopting the preparation method disclosed by the invention have the property that the charge carrying property can beadjusted by means of the pH value, that is to say, the hyaluronic acid is ionized to carry negative charges under an alkaline condition, and the chitosan is protonized to carry positive charges underan acidic condition. The nanofibers have good application prospects in the aspects of biological tissue engineering, medicament or gene carrier materials, biomedical materials and the like.

Description

technical field [0001] The invention relates to a preparation method of natural product-based polyelectrolyte composite nanofibers, in particular to a preparation method of hyaluronic acid and chitosan composite polyelectrolyte nanofibers. [0002] Background technique [0003] Electrospinning technology is a simple and effective method to prepare nanofibers. It is a method of preparing polymer microfibers by jetting charged polymer solution or melt in an electrostatic field. When the applied electric field reaches a certain critical value, the jet will be ejected from the spinneret, and at the same time the solvent will volatilize, and the jet will dry and solidify to form ultrafine fibers. The properties of the solution (such as: surface tension, conductivity, viscosity, etc.) have an important impact on its spinnability. Due to its high specific surface area, high porosity, and biomimetic advantages, electrospun fiber membranes have high and wide application value in b...

Claims

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Application Information

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IPC IPC(8): D01F8/18D01D5/00
Inventor 马贵平聂俊方大为朱晓群
Owner BEIJING UNIV OF CHEM TECH
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