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Kit used for diagnosing immune associated diseases of human or animals

A correlation and kit technology, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as lack of treatment methods and unsatisfactory vaccine prevention effects

Active Publication Date: 2014-02-19
潍坊康奥思生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] So far, people still lack effective treatments for chronic infectious diseases caused by intracellular parasitic pathogens (viruses, bacteria, parasites, etc.), and vaccines The preventive effect is not ideal
Theoretically, such chronic infectious diseases require the host to produce a strong type (Th1) anti-infection immune response to eliminate pathogens, but how to induce the body to produce a strong Th1 anti-infection immune response is still a difficult problem

Method used

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  • Kit used for diagnosing immune associated diseases of human or animals
  • Kit used for diagnosing immune associated diseases of human or animals
  • Kit used for diagnosing immune associated diseases of human or animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of mouse mastocytoma (dynamic) diagnostic kit

[0022] 1. The composition of the kit: antigen, enzyme-labeled secondary antibody, ELISPOT antibody combination (including anti-cytokine primary antibody, biotin-labeled secondary antibody and enzyme-labeled avidin), positive and negative control serum, washing solution, samples Diluent, substrate solution, stop solution, special chemiluminescent reagent for WEST-BLOT (A and B) and X-ray film.

[0023] 2. Preparation method:

[0024] (1) Antigen preparation method: collect a number of mouse mast cell tumor cells, wash them twice with PBS, add an appropriate amount of PBS to resuspend the cells, lyse the cells with ultrasonic waves, and centrifuge at 5000RPM for 10 minutes to remove cell debris. Gradient centrifugation to collect proteins with a molecular weight in the range of 30-100kDa, measure the protein concentration, and if it is not less than 1mg / ml, it is the spare antigen. Then coat t...

Embodiment 2

[0043] Example 2: Using the kit prepared in Example 1, the criteria for dynamic (or early) diagnosis of cancer are determined by measuring different types of cytokines and / or antibody subtypes.

[0044] 50 mice were randomly divided into two groups, 30 in the experimental group and 20 in the control group. The mice in the experimental group were first immunized with appropriate tumor vaccines, blood samples were collected 2 weeks later to measure their immune response, and 20 mice in the immune protection state were selected for tumor attack experiments. The immunized mice and the control mice were challenged with a high dose of tumor cells, so that 100% of the control mice developed tumors, while the immunized mice were basically 100% protected with an appropriate dose. Two weeks after the high-dose tumor cell challenge, blood samples were collected, red blood cells were lysed, and white blood cells were washed and counted, which were then used to determine the levels of spec...

Embodiment 3

[0082] Example 3: An example of dynamic diagnosis of mouse cancer using the kit prepared in Example 1 and the diagnostic criteria formulated in Example 2

[0083] 30 mice were randomly divided into two groups, 20 in the experimental group and 10 in the control group. Blood was collected before the experiment, and the serum was reserved for future use. The mice in the experimental group were inoculated with an appropriate amount of tumor cells intradermally (i.d.) for about 2 weeks. When the tumor grew to a diameter of about 3mm, the tumor was surgically removed, and blood was collected at an appropriate interval after the operation, and the serum was separated to measure the specificity. Sexual antibody subclasses IgG1 and IgG2a, the method is indirect ELISA or WEST-BLOT assay method (see Example 2 for details), and ends after 85 weeks.

[0084] See the experimental results image 3 . It can be seen from the figure that both IgG1 and IgG2a were negative at -2 weeks, indicat...

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Abstract

The invention discloses a kit used for diagnosing the immune associated diseases of human or animals, and the kit comprises antibodies or cell factors, antibody antigens or the stimulation sources of the cell factors, and other reagents required by forming the kit, wherein the antibodies are two or more than two antibodies or sub-antibodies with the function of distinguishing different immune response types; and the cell factors are two or more than two cell factors with the function of distinguishing different immune response types. In the kit, a special biomarker serves as a diagnosis indicator to realize the purpose of diagnosis and the purpose of distinguishing different immune response types of the first class, the second class and the like. The diagnosis has the important meaning that the disease developing degree can be identified by the diagnosis indicator at the same time; and according to the dynamic variation of the indicator in a therapeutic process, clinicians can be correctly guided to regulate a therapeutic scheme in time, thereby properly treating diseases, and making a correct judgement for prognosis.

Description

technical field [0001] The invention relates to a kit for diagnosing immune-related diseases in humans or animals. Background technique [0002] So far, people still lack more effective treatment methods for chronic infectious diseases caused by intracellular parasitic pathogens (viruses, bacteria, parasites, etc.), and the preventive effect of vaccines is not satisfactory. Theoretically, such chronic infectious diseases require the host to produce a strong type (Th1) anti-infection immune response to eliminate pathogens, but how to induce the body to produce a strong Th1 anti-infection immune response is still a difficult problem. Contents of the invention [0003] Aiming at the above-mentioned prior art, the present invention provides a kit that can be used for (dynamic) diagnosis of human or animal immune-related diseases and correct guidance of clinical treatment. [0004] The present invention is achieved through the following technical solutions: [0005] A kit for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/531
Inventor 刘永庆
Owner 潍坊康奥思生物技术有限公司
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