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Application of dog liver vegetable polysaccharide

A dog liver vegetable and polysaccharide technology, applied in the field of medicine, can solve the problems of polysaccharides in vivo process, unknown mechanism of action, unclear relationship between polysaccharide structure and function, difficult quality control, etc., to improve complex ingredients, improve drug effect repeatability and quality stability Sexuality and the effect of reducing the preparation cost

Inactive Publication Date: 2011-12-21
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been found that polysaccharides from dog liver vegetables have hepatoprotective effect, but the research reports in the prior art basically focus on the research on crude polysaccharides from dog liver vegetables. Due to the complex composition and low content of crude polysaccharides from dog liver vegetables, and the relationship between the structure and function of polysaccharides is not clear, polysaccharides The in vivo process and mechanism of action are unknown, and the quality is difficult to control, resulting in poor reproducibility of the drug effect, and it is difficult to enter the international market

Method used

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  • Application of dog liver vegetable polysaccharide
  • Application of dog liver vegetable polysaccharide
  • Application of dog liver vegetable polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042]Weigh 1 kg of dry whole grass powder of dog liver dish, add appropriate amount of petroleum ether to degrease, remove impurities with absolute ethanol, and reflux each for extraction for 2 hours. The dregs were extracted by boiling water for 2 times, each time for 2 hours, and the extracts were combined, concentrated to 800mL, extracted with chloroform-n-butanol (4:1, volume ratio) multiple times (refer to the Sevage method), and the protein was removed. Ethanol with a volume ratio concentration of 95% makes the alcohol content reach 80% (volume ratio concentration), and alcohol precipitation is carried out in a refrigerator at 4° C. for 12 hours. After suction filtration, the filter residue was washed with absolute ethanol, and dried under vacuum at 50°C for 4 hours to obtain the total polysaccharide of dog liver dish with a yield of about 9%.

[0043] Take 70g of the total polysaccharide of dog liver dish, dissolve it in 150mL of purified water, make a dialysis bag wit...

Embodiment 2

[0046] Example 2 The application experiment of different molecular weight polysaccharide groups of dog liver dish of the present invention

[0047] Male SD rats (220±20g) were provided by the Experimental Animal Center of Guangdong Province (certificate number: SCXK Yue 2008-0002). The rats were randomly divided into 7 groups (10 rats in each group), maintained a 12-hour circadian rhythm, controlled humidity and air circulation, had free access to water and food, and were adaptively fed for 7 days before the experiment.

[0048] 70 male SD rats were randomly divided into 7 groups, 10 in each group, which were normal control group, liver injury model group, bifendate group, dog liver vegetable polysaccharide group, dog liver vegetable polysaccharide group with a molecular weight less than 6000, and a dog liver vegetable polysaccharide group with a molecular weight of 6000~ 10000 dog liver vegetable polysaccharide group and dog liver vegetable polysaccharide group with molecular...

Embodiment 3

[0059] Example 3 The application experiment of different molecular weight polysaccharide groups of dog liver dish of the present invention

[0060] Experimental method is the same as embodiment 2. The experimental results are shown in Table 2:

[0061] Table 2 Effects of various parts of dog liver vegetable polysaccharides on liver tissue enzymes (n=10)

[0062]

[0063] p value: with embodiment 2.

[0064] The experimental results shown in Table 2 show that there is a significant difference between the liver injury model group and the blank control group, indicating that the experimental model is successful. Compared with the model group, the positive control drug significantly enhanced the reduction of SOD and GSH-Px enzyme activities caused by liver damage (P<0.01).

[0065] For SOD enzyme activity, the total polysaccharide group significantly enhanced the decrease in SOD enzyme activity due to liver damage (P10000.

[0066] For GSH-Px enzyme activity, the total poly...

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Abstract

The invention discloses application of dicliptera chinensis polysaccharide. According to the application, the liver protecting and enzyme reducing activities of polysaccharide with different molecular weights are researched and summarized, particularly including application of a polysaccharide group with the molecular weight of less than 6000 and a total polysaccharide group to preparation of a medicament for treating TP (Total Protein) rise and / or SOD (Superoxide Dismutase) enzymatic activity reduction symptom caused by liver damage or health-care products, application of the polysaccharide group with the molecular weight of less than 6000 and the total polysaccharide group to preparation of a medicament for treating GSH-Px (Glutathione Peroxidase) enzymatic activity reduction symptom caused by liver damage or health-care products, and the like. According to the application disclosed by the invention, the phenomena of complex components and lower content of crude polysaccharides are improved and the relation between the molecular weight composition and the functions of polysaccharide is summarized. The dicliptera chinensis can be applied to preparation of a medicament for protecting liver and reducing enzyme. The product prepared by the invention is easy to dissolve and simultaneously retains the liver protecting physiologically activity of the dicliptera chinensis polysaccharide; and the product has high yield, high content and low preparation cost.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of dog liver vegetable polysaccharide and its application in the preparation of medicines for treating liver damage. Background technique [0002] Liver injury is the liver damage caused by the invasion of external factors. Hepatitis virus can cause liver damage, but not all liver damage must be hepatitis. Liver injury is mainly caused by violent liver injury, pathological liver injury, chemical liver injury and other reasons. The symptoms of violent liver injury are obvious and severe quickly; pathological liver injury and chemical liver injury are relatively common, but the development of pathological and chemical liver injury is relatively slow. [0003] There are more and more patients with liver damage caused by pathogens, chemical drugs or excessive drinking. There are more than ten million patients with liver damage in my country. Modern...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P1/16A23L1/30
Inventor 沈志滨崔红花陈艳芬尹永芹张鑫
Owner GUANGDONG PHARMA UNIV
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