Method for preparing memory-improving bioactive peptide from deep sea fish
A technology for bioactive peptides and memory improvement, which is applied in the field of preparation of bioactive peptides to achieve the effects of improving the ability of the human body, improving memory, and simple preparation process
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Embodiment 1
[0035] (1) Restricted enzymatic hydrolysis: After the small yellow croaker is caught, it is frozen at -18oC. After thawing, the head and viscera are removed, and the meat is minced for later use. Use the above minced meat as raw material, add water to make the protein content reach 10% (w / w), heat in a water bath to 45oC, add Alcalase to enzymatically hydrolyze until the degree of hydrolysis is 28%, inactivate the enzyme at 98oC for 10 minutes, and obtain the enzymolyzate;
[0036] (2) Separation of protein and lipid: the enzymatic hydrolyzate was refrigerated and centrifuged at 4 oC, the centrifugation speed was 4800g, and the centrifugation time was 30min. After centrifugation, the upper layer of lipid was removed to obtain the supernatant;
[0037] (3) Ultrafiltration to remove macromolecular proteins; filter the centrifuged supernatant through an ultrafiltration membrane with a molecular weight of 10kDa, and take the permeate;
[0038] (4) Ion exchange resin separation: Pa...
Embodiment 2
[0041] (1) Restricted enzymatic hydrolysis: After the grouper is caught, it is frozen at -22oC. After thawing, the head and viscera are removed, and the meat is minced for later use. Use the above minced meat as raw material, add water to make the protein content reach 6% (w / w), heat in a water bath to 60oC, add papain to enzymatically hydrolyze until the degree of hydrolysis is 35%, inactivate the enzyme at 90oC for 15 minutes, and obtain the enzymatic solution;
[0042] (2) Separation of protein and lipid: the enzymatic solution was refrigerated and centrifuged at 8 oC, the centrifugation speed was 6500g, and the centrifugation time was 45min. After centrifugation, the upper layer of lipid was removed to obtain the supernatant;
[0043] (3) Remove macromolecular proteins by ultrafiltration; filter the centrifuge supernatant through an ultrafiltration membrane with a molecular weight of 30kDa, and take the permeate;
[0044] (4) Ion exchange resin separation: Pass the permeat...
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