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Preparation method for 17 beta-estradiol molecular imprinting film electrochemical sensor

A membrane electrochemical and molecular imprinting technology, applied in the field of analysis and detection, can solve problems such as long elution time

Inactive Publication Date: 2012-01-18
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the elution time of this method is longer

Method used

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  • Preparation method for 17 beta-estradiol molecular imprinting film electrochemical sensor
  • Preparation method for 17 beta-estradiol molecular imprinting film electrochemical sensor
  • Preparation method for 17 beta-estradiol molecular imprinting film electrochemical sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] (1) Glassy carbon electrode pretreatment

[0081] Glassy carbon electrode (Φ=3mm) through 0.05μm Al 2 o 3 After the suspension was polished, put into Piranha solution (30% H 2 o 2 : Concentrated H 2 SO 4 =3:7, V / V) for 2 minutes, and then ultrasonically cleaned with absolute ethanol and deionized water for 2 minutes.

[0082] (2) Nano-Pt particle modified glassy carbon electrode

[0083] Put the polished and cleaned glassy carbon electrode (GCE) in 0.5mol·L -1 h 2 SO 4 +5mmol·L -1 h 2 PtCl 6 In the solution, within the potential range of -0.2 ~ 0.6V, at 50mV s -1 Electrodeposited Pt at a scanning rate of 10 minutes, then rinsed with absolute ethanol and deionized water in sequence, dried with nitrogen, then soaked in Piranha solution for 5 minutes, rinsed with deionized water, and dried with nitrogen to obtain Platinum nanoparticles modified glassy carbon electrodes (PtNPs / GCE), stored in PBS solution.

[0084] (3) Molecular self-assembled membrane modifie...

Embodiment 2

[0093] (1) Glassy carbon electrode pretreatment

[0094] Glassy carbon electrode (Φ=3mm) through 0.05μm Al 2 o 3 After the suspension was polished, put into Piranha solution (30% H 2 o 2 : Concentrated H 2 SO 4 =3:7, V / V) for 5 minutes, and then ultrasonically cleaned with absolute ethanol and deionized water for 2 minutes.

[0095] (2) Nano-Pt particle modified glassy carbon electrode

[0096] Put the polished and washed GCE in 0.5mol·L -1 h 2 SO 4 +5mmol·L -1 h 2 PtCl 6 In the solution, within the potential range of -0.2 ~ 0.6V, at 50mV s -1 The scan rate was cyclically scanned for 10 min to electrodeposit Pt, then rinsed with absolute ethanol and deionized water in sequence, and dried with nitrogen. Then soak in Piranha solution for 8 minutes, rinse with deionized water, and blow dry with nitrogen to obtain platinum nanometer modified glassy carbon electrodes (PtNPs / GCE), which are stored in PBS solution.

[0097] (3) Molecular self-assembled membrane modified...

Embodiment 3

[0104] (1) Glassy carbon electrode pretreatment

[0105] Glassy carbon electrode (Φ=3mm) through 0.05μm Al 2 o 3 After the suspension was polished, put into Piranha solution (30% H 2 o 2 : Concentrated H 2 SO 4 =3:7, V / V) for 3 minutes, and then ultrasonically cleaned with absolute ethanol and deionized water for 2 minutes each.

[0106] (2) Nano-Pt particle modified glassy carbon electrode

[0107] Put the polished and washed GCE in 0.5mol·L -1 h 2 SO 4 +5mmol·L -1 h 2 PtCl 6 In the solution, within the potential range of -0.2 ~ 0.6V, at 50mV s -1 The scan rate was cyclically scanned for 28min to electrodeposit Pt, and then rinsed with absolute ethanol and deionized water, respectively, and dried with nitrogen. Soak in Piranha solution for 3 minutes, rinse with deionized water, and blow dry with nitrogen to obtain platinum nanometer-modified glassy carbon electrodes (PtNPs / GCE), which are stored in PBS solution.

[0108] (3) Molecular self-assembled membrane mod...

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Abstract

The invention discloses a preparation method for a 17 beta-estradiol molecular imprinting film electrochemical sensor. In the method, the 17 beta-estradiol molecular imprinting film electrochemical sensor is prepared through the steps in sequence as follows: pretreating an electrode, modifying the electrode by nanometer platinum particles, modifying the electrodes by a molecular self-assembly film, carrying out an electropolymerization reaction and eluting template molecules. The molecular imprinting electrochemical sensor prepared by the invention has high selectivity, rapid response, high sensitivity, high stability and high tolerance, is capable of realizing high-efficiency, sensitive and real-time detection on trace 17 beta-estradiol in a water sample and has an important significance in monitoring environmental pollutants.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a preparation method of a 17β-estradiol molecularly imprinted membrane electrochemical sensor. Background technique [0002] With the development of human production activities, a large number of harmful chemical substances continue to enter the human living environment, and the residue of environmental estrogen has caused great harm to human health. As early as the early 1990s, the influence of environmental estrogen on human reproduction and development and its relationship with hormone-dependent tumors and autoimmune diseases aroused the attention of society and medical circles. Among them, endogenous estrogen (estradiol) has strong physiological activity, and even at extremely low concentrations (mostly at the ppt-ppb level or lower) will have a great impact on organisms, so the detection of estradiol And control has become a hot issue of global co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48C25B3/00
Inventor 周学敏姜慧君李芸文婷婷袁丽华周萍王若禹吴晓丽王舒
Owner NANJING MEDICAL UNIV
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