Liquid chromatography reverse phase separation and electrochemical detection and analysis system

A liquid chromatography and analysis system technology, which is applied in the direction of material separation, material analysis, and measuring devices, to achieve the effects of easy control of conditions, high detection sensitivity, and easy operation

Inactive Publication Date: 2012-01-25
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on direct separation and electrochemical detection of am

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  • Liquid chromatography reverse phase separation and electrochemical detection and analysis system
  • Liquid chromatography reverse phase separation and electrochemical detection and analysis system
  • Liquid chromatography reverse phase separation and electrochemical detection and analysis system

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Embodiment Construction

[0026] specific implementation plan

[0027] The present invention will be further specifically described below in conjunction with the accompanying drawings of the description

[0028] In the present invention, the use of the polymer reversed-phase column can be used under alkaline conditions and the pulse amperometric detection method needs to work under alkaline conditions, and the tobramycin is retained in the polymer reversed-phase column. figure 1 It is the device diagram of the analysis method, as shown in the figure, the mobile phase is transported from the ion chromatography pump 1, the sample to be analyzed 3 is injected by the quantitative loop 4 on the six-way valve 2, and the components separated by the separation column 5 enter the pulse ampere Detector 6, the tail liquid is discharged to waste liquid tank 7, and the signal is recorded and processed by computer and workstation. figure 2 In order to use the above method to analyze the chromatogram of tobramycin...

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Abstract

The invention relates to a use system for analysis of a chemical apparatus, and in particular relates to a liquid chromatography and electrochemical analysis system for directly separating an autonomous polymer reverse phase column and electrochemically detecting aminoglycoside antibiotic. In the system, the polymer reverse phase column can be used under an alkali condition and a pulse ampere detection method can work under the alkali condition; under the alkali condition, tobramycin can be retained in the polymer reverse phase column, so a little of acetonitrile is added into a sodium hydroxide solution so as to form an elution liquid; the autonomous polymer reverse phase column with a polystyrene divinylbenzene matrix is used as a fixed phase; and the pulse ampere detection method is used for detecting the tobramycin. By using the autonomous polymer reverse phase column, the analysis cost is reduced greatly; under the alkali condition, the tobramycin can be separated directly; the pulse ampere detection method is used for detecting the tobramycin; the system has a simple structure, and a complicated derivation method is not required; an extra after-column alkalifying device is not required in the electrochemical detection; the conditions are easy to control, the operation is simple, measurement can be finished within a short time, and the detection sensitivity is high.

Description

technical field [0001] The invention relates to a system for chemical instrument analysis, in particular to a liquid chromatography-electrochemical analysis system for direct separation of self-made polymer reversed-phase columns and electrochemical detection of aminoglycoside antibiotics (tobramycin). [0002] Background technique [0003] Aminoglycoside antibiotics (Aminoglycosides) are glycoside antibiotics formed by linking aminosugar and aminocyclic alcohols through oxygen bridges. They mainly inhibit the synthesis of bacterial proteins and are used for systemic infections caused by sensitive aerobic Gram-negative and positive bacilli. . Tobramycin is one of the broad-spectrum aminoglycoside antibiotics, which has good antibacterial effect on both Gram-positive and Gram-negative bacteria. Due to the lack of ultraviolet absorption, the determination of tobramycin is mostly carried out by microbial method, optical rotation method, spectrophotometry, HPLC pre-column and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 朱作艺朱岩
Owner ZHEJIANG UNIV
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