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Compositions and methods for the production of a compound

A technology of composition and compound, applied in the field of synthetic compound, isolated composition, in vitro cell-free system, and synthetic shikimic acid, which can solve the problem that the method of influenza is not enough to deal with global or even national emergencies

Inactive Publication Date: 2012-01-25
GREENLIGHT BIOSCIENCES INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, despite advances in treatments for influenza, current treatments for influenza are insufficient for a global or even national emergency

Method used

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  • Compositions and methods for the production of a compound
  • Compositions and methods for the production of a compound
  • Compositions and methods for the production of a compound

Examples

Experimental program
Comparison scheme
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preparation example Construction

[0051] Preparation of cell extracts

[0052] In one embodiment of the invention, a cellular extract of a photosynthetic organism comprising components of a photosynthetic pathway is provided. Typically, components of the photosynthetic pathway are present in thylakoid membranes or photosynthetic bacterial membranes (such as those of cyanobacteria). Thylakoid membranes can be obtained from any plant, algae or photosynthetic bacteria. Typically, the photosynthetic organism is grown under conditions favorable for its growth prior to extraction of thylakoid membranes. Large numbers of photosynthetic organism cells can be grown in fermentors or bioreactors according to methods known in the art for growing cells. The methods and conditions used to extract thylakoid membranes preferably leave intact the photosystem components of the associated membranes. The cyclic and acyclic photosynthetic machinery of plant cells to catalyze ATP production through ferredoxin photoactivation and...

example

[0104] The present invention will be described with reference to the following examples, which are provided for purposes of illustration and are not intended to limit the invention in any way. The examples utilize standard techniques well known in the art or as specifically described below.

example 1

[0105] Example 1. Production of shikimic acid by incorporation of the photosynthetic ATP regeneration system.

[0106] The present invention provides compositions and methods for producing adenosine triphosphate (ATP) in a cell-free system and producing compounds by enzymes or enzymatic pathways in a cell-free system. Examples of general cell-free systems are provided in figure 1 middle. ATP is produced by an ATP regeneration system that catalyzes the formation of ATP from adenosine diphosphate (ADP) and inorganic phosphate. ATP regeneration systems include photosynthetic ATP regeneration systems of plants and other photosynthetic organisms. Enzymes or enzyme pathways requiring ATP can be combined with ATP regeneration systems to produce related compounds, such as shikimate. This study will evaluate the ability of isolated thylakoid fractions to generate ATP in vitro. This study will evaluate the rate and yield of ATP production and shikimic acid production in a cell-free...

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Abstract

The invention features compositions and methods that are useful for the production of a compound.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of US Provisional Application Serial No. 61 / 140,053, filed December 22, 2008, which is hereby incorporated by reference in its entirety. Background technique [0003] Despite advances in industrial fermentation and advances in biotechnology, current methods still do not allow large-scale (industrial or commercial) batch manufacturing of certain compounds. For example, most linear and aromatic hydrocarbons produced, eg, during the manufacture of biofuels, are strong inhibitors of cell growth. Thus, biochemical synthesis is limited to natural products (eg, vitamins, enzymes, antibodies) as well as high-value, small-volume synthetics. Nevertheless, there is still a large market for carbon-neutral synthetic products and cytotoxic products that cannot be produced by industrial fermentation. Therefore, alternative manufacturing methods that enable large-scale production of difficult-to-sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00
CPCC12P21/02C12P7/42C12N9/00A61P31/16C12N9/90C12P1/00C12Y207/01001
Inventor 安德雷·J·扎鲁尔詹姆斯·斯沃茨
Owner GREENLIGHT BIOSCIENCES INC