Method for determining starch content of cane juice or syrup
A technology of starch content and determination method, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of cumbersome steps, large error, poor reproducibility, etc., and achieve the effect of simple operation, small error, fast and accurate determination method
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Embodiment 1
[0035] A method for assaying starch content in sugarcane juice, comprising the following steps:
[0036] 1) Heat and boil 100ml of sugarcane juice filtered through an 80-mesh sieve for 15 minutes, and then filter to obtain clear sugarcane juice;
[0037] 2) Take 20ml of sugarcane juice and add 100μl of high-temperature amylase to it, and react in a water bath at 80°C for 20min;
[0038] 3) Add 2ml of the clear solution in step 2) and 1ml of 1mol / L hydrochloric acid to six 25ml colorimetric tubes, and mix well;
[0039] 4) Then add 0 μl, 80 μl, 160 μl, 240 μl, 320 μl, 400 μl of starch solution with a concentration of 1000 μg / mL to the 6 colorimetric tubes;
[0040] 5) Add 5ml of iodine solution to the series of colorimetric tubes, and then add water to make up the volume to 10ml, wherein the solutes in the iodine solution are 15mmol / L KI and 5mmol / L I 2 ;
[0041] 6) Measure the absorbance of starch in the series of mixtures obtained in step 5) at a wavelength of 580nm, and ...
Embodiment 2
[0048] A method for assaying starch content in sugarcane juice, comprising the following steps:
[0049] 1) Heat and boil 100ml sugarcane juice filtered through a 90-mesh sieve for 15 minutes, and then filter to obtain clear sugarcane juice;
[0050] 2) Take 20ml of sugarcane juice, add 120μl of high-temperature amylase to it, and react in a water bath at 70°C for 10min;
[0051] 3) Add 1.6ml of the clear solution in step 2) and 0.8ml of 1mol / L hydrochloric acid to six 25ml colorimetric tubes, and mix well;
[0052] 4) Then add 0μl, 80μl, 160μl, 240μl, 320μl, 400μl starch solutions with a concentration of 800μg / mL to the 6 colorimetric tubes;
[0053] 5) Add 4ml of iodine solution to the series of colorimetric tubes, and then add water to make up the volume to 10ml, wherein the solutes in the iodine solution are 15mmol / L KI and 5mmol / L I 2 ;
[0054] 6) Measure the absorbance of starch in the series of mixtures obtained in step 5) at a wavelength of 580nm, and draw a workin...
Embodiment 3
[0061] A method for assaying starch content in sugarcane juice, comprising the following steps:
[0062] 1) Heat and boil 100ml of cane juice filtered through a 100-mesh sieve for 10 minutes, and then filter to obtain clear cane juice;
[0063] 2) Take 20ml of sugarcane juice and add 140μl of high-temperature amylase to it, and react in a water bath at 90°C for 10min;
[0064] 3) Add 1.8ml of the clear solution in step 2) and 0.9ml of 1mol / L hydrochloric acid to six 25ml colorimetric tubes, and mix well;
[0065] 4) Then add 0 μl, 80 μl, 160 μl, 240 μl, 320 μl, 400 μl of starch solution with a concentration of 900 μg / mL to the 6 colorimetric tubes;
[0066] 5) Add 4.5ml of iodine solution to the series of colorimetric tubes, and then add water to make up the volume to 10ml, wherein the solutes in the iodine solution are 15mmol / L KI and 5mmol / L I 2 ;
[0067] 6) Measure the absorbance of starch in the series of mixtures obtained in step 5) at a wavelength of 580nm, and draw ...
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