Method for evaluating eye irritation by using miniature pig erythrocytes
A system evaluation, miniature pig technology, applied in the direction of color/spectral property measurement, material inspection products, biological testing, etc., can solve problems such as animal suffering, lack of relevant patents and literature reports, and differences in animal and human responses, achieving sensitive High degree, meet the needs of routine toxicity detection and mechanism research, and have good correlation effect
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Embodiment 1
[0187] 1. Preparation of Minipig Red Blood Cell Suspension
[0188] 1.1 Miniature pigs
[0189]The blood comes from closed group Wuzhishan miniature pigs, and the anticoagulant blood comes from Wuzhishan miniature pigs raised in a standardized manner. The genetic background is clear, the population is stable, and the source is reliable, which can meet the needs of testing. The healthy and stable source of red blood cells; , age and weight are uniformly controlled, and the difference between batches is small to ensure stable and reliable test results; the damage effect of chemicals on the cell membrane is quantitatively evaluated by red blood cell hemolysis; A predictive model was established to evaluate the eye irritation of chemical substances.
[0190] 1.2 Preparation of minipig red blood cell suspension
[0191] 1.2.1 Experimental materials
[0192] PBS buffer solution, PBS buffer solution contains the following components: based on 1L of buffer solution, sodium chl...
Embodiment 2
[0226] 1. Preparation of Minipig Red Blood Cell Suspension
[0227] 1.1 Miniature pigs
[0228] The blood material comes from a closed herd of Wuzhishan miniature pigs native to Wuzhishan District, Hainan Province.
[0229] 1.2 Preparation of minipig red blood cell suspension
[0230] 1.2.1 Experimental materials
[0231] PBS buffer solution: pH 7.3, stored at 4°C, PBS buffer solution contains the following components: based on 1L of buffer solution, sodium chloride 8.0g, potassium chloride 0.2g, glucose 1.8g, anhydrous disodium hydrogen phosphate 1.15g, Anhydrous potassium hydrogen phosphate 0.2 g, the rest is deionized water.
[0232] 5mL anticoagulant tube: containing K 2 -EDTA.
[0233] Positive control solution: Prepare a sodium dodecyl sulfate (SDS) solution with a concentration of 10 g / L in PBS.
[0234] 1.2.2 Test substance: Benzophenone is a fragrance fixative, which can be used as a raw material for cosmetics, and can also be used to manufacture antihistamines...
Embodiment 3
[0264] 1. Preparation of Minipig Red Blood Cell Suspension
[0265] 1.1 Miniature pigs
[0266] Tibetan miniature pigs are native to the Nyingchi region of Tibet and later turned into experimental animals. They are standardized experimental animals with known genetic background, clear microbial background, and stable population.
[0267] 1.2 Preparation of minipig red blood cell suspension
[0268] 1.2.1 Experimental materials
[0269] PBS buffer solution: pH 7.2, stored at 4°C, PBS buffer solution contains the following components: based on 1L buffer solution, 8.0g sodium chloride, 0.2g potassium chloride, 1.8g glucose, 1.15g anhydrous disodium hydrogen phosphate, Anhydrous potassium hydrogen phosphate 0.2 g, the rest is deionized water.
[0270] 5mL anticoagulant tube: containing citrate buffer, in which the concentration of sodium citrate in the citrate buffer is 66 mmol / L, and the concentration of citric acid is 44 mmol / L, prepared with ultrapure water.
[0271] Posit...
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