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Extraction method for diverse wormwood herb and application of extractive of diverse wormwood herb

An extraction method and an extract technology, which are applied to the Liu Jinu extraction method and the application field of the extract in cosmetics

Inactive Publication Date: 2012-03-14
SHANGHAI BOTANOVA COSMETIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report in the existing literature that the extract of Liu Jinu and its derivatives and analogs have the activity of inhibiting TNFα production

Method used

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  • Extraction method for diverse wormwood herb and application of extractive of diverse wormwood herb
  • Extraction method for diverse wormwood herb and application of extractive of diverse wormwood herb
  • Extraction method for diverse wormwood herb and application of extractive of diverse wormwood herb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1-Preparation of Liu Jinu ethanol extract

[0018] The ethanol extract of Liu Jinu mentioned in the present invention is extracted from the dried whole plant of Liu Jinu of the Compositae family through multiple reflux extraction with organic solvents. The components extracted from the solvent are further purified by chromatography or fractionation, and the product is separated.

[0019] The pulverized particle size of the Liu Jinu is 100um-5000um; it is extracted by heating and refluxing with 30-90% ethanol aqueous solution; the ratio of medicinal materials and solvent is 1:10; the extraction temperature is kept between 20-90℃; the extraction time is 8 Between -24 hours; the ethanol extract is decolorized by 1-40% activated carbon and concentrated for later use. Process flow like figure 1 Shown.

[0020] The sample liquid was further concentrated and dried to prepare Liu Jinu ethanol extract. The appearance of the product is brown powder (it can also exist in the...

Embodiment 2

[0023] Example 2 Experimental study on the inhibition of the precursor inflammatory factor TNFa extracted by Liu Jinu

[0024] Experimental Materials :

[0025] 1. Cells: normal human peripheral blood mononuclear cells (PBMC)

[0026] 2. Tested drug: Liu Jinu ethanol extract

[0027] 3. Positive control: dexamethasone (product of Sigma, USA)

[0028] 4. Reagents: Ficoll-Paque Plus (Amersham Bioscience); endotoxin (LPS, lipopolysaccharide) and dexamethasone (CalBiochem.); TNFa ELISA Kit (Jingmei Bioengineering Company); dimethyl sulfoxide (DMSO) for Sigma Company's products; cell culture media and fetal bovine serum Gibco's products

[0029] experiment method:

[0030] Fresh blood uses EDTA as anticoagulant, Ficoll separates blood cells, and resuspends the cells in RIMP 1640 medium containing 10% fetal bovine serum. Add 100 ??l to a 96-well plate with a density of 1×10 5 Cells / ml newly isolated cells, the total number of cells per well is 10 4 Each sample makes 3 holes.

[0031] 1) Wei...

Embodiment 3

[0043] Example 3: Preparation of skin cream (by weight ratio)

[0044] Liu Jinu ethanol extract 0.1-3%

[0045] Glycerin 3-8%

[0046] Caprylic acid / capric acid / triglyceride 3-8%

[0047] Isooctyl palmitate 3-8%

[0048] Cetyl Alcohol / Stearyl Alcohol 3-5%

[0049] C12-20 alkyl glucoside 1-3%

[0050] Ceteareth-25 1-3%

[0051] Polydimethylsiloxane 1-3%

[0052] Mineral oil 3-8%

[0053] Methyl paraben 0.1-0.5%

[0054] Phenoxyethanol 0.3-0.5%

[0055] Flavor amount

[0056] Deionized water plus 100%

[0057] Preparation method: Heat the glycerin and methyl paraben in the de-particle water and water phase to 80°C, and simultaneously heat the oil phase isooctyl palmitate, caprylic acid / capric acid / triglyceride, cetyl alcohol / stearyl alcohol, C12-20 alkyl glucoside, ceteareth-25, polydimethylsiloxane, and phenoxyethanol are heated to 80°C, and the oil phase solution is slowly added to the water phase solution while stirring, homogenization Stir for 2-5 minutes, turn on the vacuum, stir and keep f...

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Abstract

The invention relates to the cosmetic industry and especially to an extraction method for diverse wormwood herb and application of the extractive of diverse wormwood herb in cosmetics. According to the invention, the compositae of diverse wormwood herb is dried, and integral diverse wormwood herb with flowers is subjected to mechanical grinding and crushing to prepare powder; the powder is subjected to reflux extraction with an organic solvent a plurality of times; the ingredients obtained after solvent extraction are further purified by using the method of chromatography or fractionation for separation to obtain the extractive of diverse wormwood herb after separation. The extractive of diverse wormwood herb in the invention can be used as a natural plant additive in cosmetics for anti-inflammation, whitening or freckle removing for skin.

Description

technical field [0001] The invention relates to the cosmetics industry, in particular to an extraction method of Liu Jinu and the application of the extract in cosmetics. Background technique [0002] Cosmetics are very popular commodities, and their consumption follows the social trend, which has distinct characteristics of the times. With the deepening of medical research, consumers are more cautious about cosmetics made of chemical compounds. In recent years, the production of cosmetics has begun to use natural raw materials. People hope to improve product safety and efficacy by adding active ingredients from natural animals and plants. [0003] Skin is an important part of maintaining human life and health. It is in direct contact with the outside world and is the first barrier of the body. It is extremely vulnerable to damage caused by the environment. In addition to the aging factors brought about by age, stress reactions such as inflammation and allergies, pollution...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61Q1/12A61Q17/04A61Q19/00A61Q19/10
Inventor 束放
Owner SHANGHAI BOTANOVA COSMETIC
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