Preparation method of temperature-sensitive cellulose quaternary ammonium salt/beta-sodium glycerophosphate hydrogel

A technology of sodium glycerophosphate and cellulose is applied in the field of materials and tissue engineering, and achieves the effects of simple method, good biocompatibility and low cost of raw materials

Active Publication Date: 2012-05-02
HUBEI GEDIAN HUMANWELL PHARMA EXCIPENTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the preparation method of cellulose

Method used

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  • Preparation method of temperature-sensitive cellulose quaternary ammonium salt/beta-sodium glycerophosphate hydrogel
  • Preparation method of temperature-sensitive cellulose quaternary ammonium salt/beta-sodium glycerophosphate hydrogel
  • Preparation method of temperature-sensitive cellulose quaternary ammonium salt/beta-sodium glycerophosphate hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Configured with 8wt% LiOH·H 2 O, 550 mL of an aqueous solution of 15 wt % urea, pre-cooled to -10 ° C, added 11 grams of cellulose at room temperature, and mechanically stirred for 3 to 5 minutes to obtain a cellulose solution (2 wt %). 111.0 g of 3-chloro-2-hydroxypropyltrimethylammonium chloride aqueous solution (69 wt %) was added dropwise to the cellulose solution, stirred for 24 hours, and then HCl was added to neutralize the reaction solution. The reaction solution was dialyzed with distilled water for 7 days and freeze-dried to obtain a white fibrous cellulose quaternary ammonium salt sample (substitution degree DS=0.34, nitrogen content 2.23%).

[0028] Dissolve 250 mg of cellulose quaternary ammonium salt (DS=0.34) in 8.5 mL of 0.1 mol / L acetic acid aqueous solution and mix evenly to form a 2.9 wt % solution. Dissolve 650 mg of β-sodium glycerophosphate in 1.5 mL of deionized water to form a 43.3 wt % solution. At 15°C, add 1.5mL of β-sodium glycerophosphate ...

Embodiment 2

[0030] According to the method of Example 1, a white fibrous cellulose quaternary ammonium salt sample with a degree of substitution DS=0.34 was prepared.

[0031] Dissolve 250 mg of cellulose quaternary ammonium salt (DS=0.34) in 8.5 mL of 0.1 mol / L acetic acid aqueous solution and mix evenly to form a 2.9 wt % solution. Dissolve 760 mg of sodium β-glycerophosphate in 1.5 mL of deionized water to form a 50.7 wt % solution. In an ice-water bath at 4°C, add 1.5mL of β-sodium glycerophosphate solution dropwise to 8.5mL of cellulose quaternary ammonium salt solution, and stir thoroughly to obtain a cellulose quaternary ammonium salt / β-sodium glycerophosphate solution, and the final mixed solution The concentration of β-glycerophosphate sodium in the medium is 7.6wt%, the concentration of cellulose quaternary ammonium salt is 2.5wt%, and when it is placed in a water bath at 37°C, the solution will quickly solidify within 30 seconds to form a hydrogel.

Embodiment 3

[0033] According to the method of Example 1, a white fibrous cellulose quaternary ammonium salt sample with a degree of substitution DS=0.34 was prepared.

[0034] Dissolve 400 mg of cellulose quaternary ammonium salt (DS=0.34) in 8.5 mL of 0.1 mol / L acetic acid aqueous solution and mix well to form a 4.7 wt % solution. Dissolve 300mg of β-sodium glycerophosphate in 1.5mL of deionized water to prepare a 20wt% solution. In an ice-water bath at 4°C, add 1.5mL of β-sodium glycerophosphate solution dropwise to 8.5mL of cellulose quaternary ammonium salt solution, and stir thoroughly to obtain a cellulose quaternary ammonium salt / β-sodium glycerophosphate solution, and the final mixed solution The concentration of β-glycerophosphate sodium in the medium is 3wt%, and the concentration of cellulose quaternary ammonium salt is 4wt%. After standing still in a water bath at 37°C, the solution will quickly solidify within 30 seconds to form a hydrogel.

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Abstract

The invention which discloses a preparation method of a temperature-sensitive cellulose quaternary ammonium salt/beta-sodium glycerophosphate hydrogel belongs to fields of the biological technology and tissue engineering. A 10-60wt% sodium glycerophosphate solution is added to a 1.5-5.5wt% cellulose quaternary ammonium salt solution drop by drop at 0-20DEG C to prepare a cellulose quaternary ammonium salt/beta-sodium glycerophosphate mixed solution, and the mixed solution is rapidly cured at the body temperature to form the hydrogel. The liquid state of the cellulose quaternary ammonium salt/beta-sodium glycerophosphate solution can be maintained at room temperature, so it is very convenient to add cells, proteins, enzymes and therapeutic medicines to the solution, thereby the obtained solution can be used as a cytoskeleton material or a medicine carrier. The cellulose derivative which has very good biological compatibility is very suitable for the fields of the biological technology and tissue engineering.

Description

technical field [0001] The invention relates to a preparation method of temperature-sensitive cellulose quaternary ammonium salt / beta-glycerophosphate sodium hydrogel, belonging to the field of materials and tissue engineering. technical background [0002] In recent years, more and more researchers have paid attention to thermosensitive hydrogels due to their outstanding advantages in drug loading, cell culture and tissue engineering. This solution is a flowable liquid prior to treatment, and when injected into the target site, it rapidly solidifies to form a hydrogel in a physiological environment. Thermosensitive hydrogels have many advantages in the application of biomedicine: 1) Due to the good fluidity of the solution before injection, it is very convenient to add drugs, growth factors and cells into the solution; 2) It can be passed through a very The solution is injected into the lesion through a small wound; 3) The solution solidifies rapidly at the lesion to form ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L1/08C08K5/521C08B15/06
Inventor 周金平尤俊张俐娜
Owner HUBEI GEDIAN HUMANWELL PHARMA EXCIPENTS
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