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Method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement

A technology for determining the method of chlorophyll fluorescence, which is applied in the field of determination of actinic light intensity in the measurement of chlorophyll fluorescence induction curve, can solve the problems of low degree of decline and unrepresentative test results, etc., and achieve simple implementation, wide application range and operability strong effect

Active Publication Date: 2013-02-13
上海乾菲诺农业科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the Y(II) of maize in the field (higher light intensity) will be significantly reduced after drought stress, but if a lower actinic light intensity is used to measure the chlorophyll fluorescence induction curve, the degree of Y(II) decline obtained Far below the actual level in the field, it will lead to unrepresentative test results

Method used

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  • Method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement
  • Method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement
  • Method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement

Examples

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Embodiment 1

[0058] figure 2 It is the chlorophyll fluorescence induction curve of the pothos radish in one embodiment of the present invention. Take a healthy live pothos (Scindapsus aureun) (Fv / Fm>0.8), clamp the leaves with a dual-channel PAM-100 measuring system DUAL-PAM-100 (Walz, Germany), and cover the whole leaves with a black cloth. Set 10 light intensity gradients from low to high in the instrument software DualPAM v1.9 (9, 89, 127, 211, 342, 522, 696, 861, 1064 and 1323 μmol m -2 the s -1 ), and set the illumination time of each light intensity gradient to 20s, and then measure the fast light curve according to the conventional method. After the measurement, use the equation P=Pm (1-e -α·PAR / Pm ) e -β·PAR / Pm Non-linear fitting is carried out to obtain the half-saturated light intensity Ik = 142μmol m -2 the s -1 . Select the light intensity closest to Ik in the light intensity list, 127 μmol m -2 the s -1 As the actinic light intensity, and then measure the chlorophyll...

Embodiment 2

[0060] image 3 It is the chlorophyll fluorescence induction curve of the sorrel under different actinic light intensities according to one embodiment of the present invention. Take healthy safflower wood sorrel (Oxalis corymbosa DC.) leaves (Fv / Fm>0.8), use the dual-channel PAM-100 measurement system DUAL-PAM-100 (Walz, Germany) to clamp the leaves, and soak the petioles in water. Cover the entire leaf with a black cloth. Set 10 light intensity gradients from low to high in the instrument software DualPAM v1.9 (49, 89, 127, 211, 342, 522, 696, 861, 1064 and 1323 μmol m -2 the s -1 ), and set the illumination time of each light intensity gradient to 20s, and then measure the fast light curve according to the conventional method. After the measurement, use the equation P=Pm (1-e -α·PAR / Pm ) e -β·PAR / Pm Non-linear fitting is carried out to obtain the half-saturated light intensity Ik = 263μmol m -2 the s -1 . Select the light intensity 127 μmol m far below Ik in the light ...

Embodiment 3

[0062] Figure 4 It is the chlorophyll fluorescence induction curve of the camphor tree of one embodiment of the present invention under different actinic light intensities. Take healthy camphor tree (Cinnamomum camphora (L.) Presl.) leaves (Fv / Fm>0.8), clamp the leaves with the leaf clip 2030-B of the portable modulation chlorophyll fluorescence instrument MINI-PAM (Walz, Germany), and soak the petioles in In water, cover the entire leaf with a black cloth. Eight light intensity gradients (116, 207, 312, 427, 620, 816, 1143 and 1504 μmol m -2 the s -1 ), and set the illumination time of each light intensity gradient to 20s, and then measure the fast light curve according to the conventional method. After the measurement, use the equation P=Pm (1-e -α·PAR / Pm ) e -β·PAR / Pm Non-linear fitting is carried out to obtain the half-saturated light intensity Ik = 286μmol m -2 the s -1 . Use the light intensity 312 μmol m closest to Ik in the light intensity list -2 the s -1 T...

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Abstract

The invention provides a method for confirming actinic light intensity in chlorophyll fluorescence induction curve measurement, which comprises the following steps of: obtaining plant leaf blades, shading environment light, setting multiple light intensity gradients, irradiating for a preset time with each light intensity gradient and measuring practical photosynthetic efficiency and relative electron transmission rate; drawing a response curve of the relative electron transmission rate, which is changed along the light intensity and fitting the response curve to obtain semi-saturated light intensity; taking the semi-saturated light intensity as the actinic light intensity for measuring the chlorophyll fluorescence induction curve; calculating a difference value between a maximal fluorescence value after light adaptation and a practical fluorescence value and a difference value between a maximal fluorescence value after dark adaptation and a basic fluorescence value after dark adaptation, and calculating the ratio of the two difference values; judging if the ratio is in a preset range, if so, confirming the current light intensity as the actinic light intensity, and if the ratio is less / more than the lower limit / the upper limit of the preset range, reducing / increasing the light intensity, re-measuring the chlorophyll fluorescence induction curve and calculating the ratio. The method can measure typical chlorophyll fluorescence induction curve.

Description

technical field [0001] The invention relates to the technical field of plant physiology, in particular to a method for determining the intensity of actinic light in the measurement of chlorophyll fluorescence induction curves. Background technique [0002] Photosynthesis is the most important chemical reaction in the process of plant physiological metabolism. It uses light energy to split water to release oxygen, and at the same time assimilate carbon dioxide to synthesize glucose, which is the basis for all life activities on earth. The measurement and research of photosynthesis has always been a hot spot in the fields of plant physiology, plant ecology, agronomy, forestry, horticulture, and algae physiology and ecology. [0003] The measurement of photosynthesis mainly includes several techniques such as gas exchange, modulation of chlorophyll fluorescence, differential absorption and photosynthetic oxygen evolution. Among them, the technology of modulating chlorophyll fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01J1/10
Inventor 韩志国胡静顾群
Owner 上海乾菲诺农业科技有限公司
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