High-density culture process of autotrophic oil-producing microalgae

A technology for high-density culture and oil-producing microalgae, which is applied in the high-density culture technology field of autotrophic oil-producing microalgae, can solve the problems of easily polluted bacteria and complex sewage components, and achieves high social and economic comprehensive benefits and comprehensive High efficiency and the effect of reducing greenhouse gas emissions

Active Publication Date: 2013-07-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The composition of sewage is complex, and the content of nutrients such as nitrogen and phosphorus is high. It is easy to contaminate bacteria (or algae) when directly used as a medium for microalgae

Method used

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  • High-density culture process of autotrophic oil-producing microalgae
  • High-density culture process of autotrophic oil-producing microalgae
  • High-density culture process of autotrophic oil-producing microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] With the municipal sewage (nitrogen content 30.0mg / L, phosphorus content 5.0mg / L) after filtration to remove miscellaneous bacteria or miscellaneous algae as the culture medium, the concentration of cultured is 4.0×10 7 cfu / ml~3.0×10 8 A high concentration of cfu / ml Chlorella autotuophica (Chlorella autotuophica) was inoculated into the photoreactor, the inoculum amount was 40% (v / v), the culture temperature was 28°C, the pH was 7.0, the light cycle was 12h, and the light intensity was 10000lux, the CO emitted by the thermal power plant 2 Exhaust gas (CO 2 The concentration is 13.0% (v / v)) as the carbon source, and the ventilation rate is 0.2VVm. Before the split culture, after 48 hours of culture, the algae concentration in the photoreactor is as high as 1.5×10 8 cfu / ml, and when the content of nitrogen and phosphorus in the photoreactor is close to the national Class A discharge water standard, the fermentation broth is divided, and 1 / 3 to 2 / 3 of the fermentation br...

Embodiment 2

[0060] With the municipal sewage (nitrogen content 30.0mg / L, phosphorus content 5.0mg / L) after filtration to remove miscellaneous bacteria or miscellaneous algae as the culture medium, the concentration of cultured is 4.0×10 7 cfu / ml~3.0×10 8 The high concentration Nannochloropsis algae (Nannochloropsissalina) of cfu / ml was inoculated into the photoreactor, the inoculation amount was 20% (v / v), the culture temperature was 20°C, the pH was 8.5, the light cycle was 16h, and the light intensity was 15000lux , the CO emitted by thermal power plants 2 Exhaust gas (CO 2 The concentration is 20.0% (v / v)) as the carbon source, and the ventilation rate is 0.1VVm. Before the split culture, after 48 hours of culture, the algae concentration in the photoreactor is as high as 3.0×10 8 cfu / ml, and when the content of nitrogen and phosphorus in the photoreactor is close to the national Class A discharge water standard, the fermentation broth is divided, and 1 / 3 to 2 / 3 of the fermentation b...

Embodiment 3

[0064] With the municipal sewage (nitrogen content 30.0mg / L, phosphorus content 5.0mg / L) after filtration to remove miscellaneous bacteria or miscellaneous algae as the culture medium, the concentration of cultured is 4.0×10 7 cfu / ml~3.0×10 8 The high-concentration Brown botrytis (Botyococcus Braunii) of cfu / ml was inoculated into the photoreactor, the inoculum size was 60% (v / v), the culture temperature was 35°C, the pH was 6.5, the light cycle was 10h, and the light intensity was 5000lux. CO emissions from thermal power plants 2 Exhaust gas (CO 2 The concentration is 10.0% (v / v)) as the carbon source, and the ventilation rate is 0.01VVm. Before the split culture, after 48 hours of culture, the algae concentration in the photoreactor is as high as 1.0×10 8cfu / ml, and when the content of nitrogen and phosphorus in the photoreactor is close to the national Class A discharge water standard, the fermentation broth is divided, and 1 / 3 to 2 / 3 of the fermentation broth is taken ou...

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Abstract

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical field of circular economy for comprehensive utilization of waste, green production for energy saving and emission reduction, in particular to the technical field of circular economy for comprehensive utilization of sewage and waste gas, and green production for energy saving and emission reduction. Density cultivation process. Background technique [0002] Energy shortage, environmental pollution and food shortage are the three major problems in the world today. Microalgae play a very important role in solving the above problems. Microalgae is a kind of microorganism capable of photosynthetic autotrophy. It is a primary producer in the ecosystem and plays a huge role in the entire material cycle. It has high production efficiency and attractive development prospects. Moreover, microalgae are rich in oil, protein, carbohydrates, trace elements and other biologically active substances, so they can not only be used...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12B01D53/84B01D53/62C02F3/32C12R1/89
CPCY02A50/20Y02P20/59
Inventor 杨海麟夏小乐辛瑜张玲王武李昌灵李宇佶
Owner JIANGNAN UNIV
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