Method for measuring distribution of human cell drug in animal body

A technology of human cells and animal bodies, applied in the biological field, can solve the problems of inability to detect the content of monkey human cells, inability to distinguish human and monkey chromosomal DNA, and narrow application range.
CN102453758AInactive Publication Date: 2012-05-16北京昭衍新药研究中心股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京昭衍新药研究中心股份有限公司
Publication Date
2012-05-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for measuring distribution of a human cell drug in an animal body, comprising the following steps of: (1) selecting a PCR(polymerase chain reaction) amplification region according to a human specific gene and designing a PCR amplification primer; (2) extracting human genome DNA(deoxyribonucleic acid), carrying out PCR amplification on a target gene fragment; (3) connecting the amplified fragment with a T vector to construct a standard plasmid; (4) establishing a standard curve by using the standard plasmid; (5) after the human cell drug is applied to an animal, collecting tissues, extracting a total DNA sample to carry out quantitative PCR reaction; and (6) calculating the contents of human genome DNA in the total DNA sample of each tissue by the standard curve, thereby calculating the contents of the human cell drug in the tissues. The measuring method provided by the invention has simple experimental processes and accurate and reliable experimental results.
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Description

Technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for determining the distribution of human cell drugs in animals. Background technique

[0002] With the development of human somatic cell research, the role of various human cells (especially mesenchymal stem cells) in the treatment of various human diseases has gradually attracted attention, such as liver damage, immune damage, nerve damage, blood For the treatment of diseases, the preclinical safety evaluation of this new type of therapeutic drug is particularly important. It is urgent to study the distribution of human cell drugs in animals after the drug.

[0003] At present, there are two main methods to study the distribution of human cell drugs in animals: one method is to modify human cell drugs (Ju Shenghong, Teng Gaojun, Mao Xi, etc., cord blood mesenchymal stem cell magnetic probe labeling and MR imaging Research, Chinese Journal of Radiology, 2005, 39: 101-106; Bos C, ...

Claims

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