Photoacoustic detection of analytes in solid tissue and detection system
A technique for analyte, in vitro detection, applied in the analysis of materials, material analysis by optical means, analysis of solids using sonic/ultrasonic/infrasonic waves, etc.
Inactive Publication Date: 2012-05-16
UNIV OF MISSOURI BOARD OF CURATORS
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Thus, only a very small proportion (sometimes less than 1%) of a typical lymph node length of 1 cm has been examined
The dyes non-selectively stain SLN
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[0013] The present invention provides methods and systems for photoacoustic detection of analytes in extracted solid tissue, such as sentinel lymph nodes. The methods and systems of the present invention enable the detection and precise localization of microscopic analytes in lymph nodes. Any analyte that is a light absorber can be detected in solid tissue, but the methods and systems of the invention are particularly useful for detecting the presence and location of micrometastases in lymph nodes. The method and system of the present invention can, for example, replace or assist conventional sentinel lymph node biopsy to detect the presence or absence of micrometastases in extracted sentinel lymph nodes and precisely determine their location in three-dimensional space.
[0014] A preferred system for in vitro detection of an analyte in solid tissue, such as an intact lymph node, comprises a laser arranged to generate a pulsed laser beam into the solid tissue, which may be an ...
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Abstract
A preferred system for detecting an analyte in solid tissue (12), such as an intact lymph node, in vitro includes a laser (22) arranged to generate a pulsed laser beam into solid tissue, which can be a fully intact lymph node. An acoustic sensor, and preferably at least three acoustic sensors (20a, 20b, 20c) are arranged in different positions to span a three dimensional space, such as in an X, Y and Z coordinate system, to detect photoacoustic signals generated within the lymph node. At least one computer (28) receives signals from the acoustic sensor(s). The computer determines the presence or absence of, and preferably the position of analyte, from the signals and the timing of the signals. A preferred method for detecting an analyte in a lymph node in vitro includes exposing an extracted lymph node to a pulsed laser beam. A photoacoustic signal is sensed.; The photoacoustic signal is analyzed to confirm the presence or absence of an analyte in the lymph node. Preferably, multiple photoacoustic signals are sensed from sensors that span a three dimensional space and the position of analyte is also determined.
Description
[0001] Priority Claims and Citations to Related Applications [0002] This application claims the benefit of an earlier provisional application, Serial No. 61 / 170,880, filed April 20, 2009, under 35 U.S.C. § 119. technical field [0003] The field of the invention is the detection of analytes in solid tissue and tissue analysis systems. A preferred application of the present invention is the in vitro detection of melanoma micrometastases in completely resected sentinel lymph nodes, and a preferred system of the present invention is a photoacoustic system that can detect the presence and location of melanoma micrometastases in extracted lymph nodes. Background technique [0004] The sentinel lymph nodes are the lymph nodes in the first group of lymph nodes reached by metastatic cancer cells traveling through the lymphatic system from a cancerous tumor. Tracing of the sentinel nodes involves detection of the sentinel nodes, typically by dye injection, and removal of the senti...
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IPC IPC(8): G01N21/17G01N33/483G01N29/04
CPCA61B8/15G01N2291/02809G01N33/4833A61B8/4477A61B5/0095A61B5/418G01N21/1702G01N29/2418A61B8/0841
Inventor J.A.维亚特P.S.戴尔D.麦克考马克
Owner UNIV OF MISSOURI BOARD OF CURATORS