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Microbial agent as well as preparation method and application thereof

A kind of microbial bacterial agent and bacterial agent technology, applied in the field of microbial bacterial agent and its preparation, can solve the problems that greenhouses cannot continue vegetable production, complicated medication, and damage to the ecological environment, so as to reduce the occurrence of subsequent crop diseases, improve soil quality, Remarkable effect

Active Publication Date: 2015-04-15
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, root-knot nematode disease and fungal disease have occurred in some vegetable greenhouses at the same time due to continuous cropping and other reasons, and the losses have been quite serious. Some greenhouses have been unable to continue vegetable production
[0003] For common root-knot nematode diseases and fungal diseases in vegetable production, chemical pesticides are usually used to prevent and control them. However, most of the chemical pesticides used at present are highly toxic and high-residue pesticides, and the medication is complicated. Nematodes and pathogenic fungi are prone to drug resistance. Pesticides It will cause serious pollution and damage to soil microorganisms, plants, water sources and the atmosphere. At the same time, pesticide residues in plants will directly cause harm to humans and other organisms and destroy the ecological environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 microbial inoculant consists of streptomyces griseus (Streptomyces griseus), bacillus subtilis (Bacillus subtilis) and bran or grass carbon soil of 10 times of two kinds of bacterium gross weights; Streptomyces griseus (Streptomyces griseus) KJA04-02, has Preserved in China Culture Collection Center on September 28, 2010, the preservation number is: CCTCC M 2010253; Bacillus subtilis (Bacillus subtilis) KJB04-11, has been preserved in China Culture Collection Center on September 28, 2010, The deposit number is: CCTCC M 2010254.

[0014] Streptomyces griseus (Streptomyces griseus) KJA04-02 strain has no septum and no breakage in the basal filaments, and the spore filaments are chain-like straight filaments. The colonies are round, with wrinkled bulges on the surface, and the edges are neatly spread radially around, powdery; the aerial hyphae are single-branched, and the spore filaments are wavy. It can liquefy gelatin, hydrolyze starch, produce hydrogen sul...

Embodiment 2

[0016] Example 2 Preparation of microbial inoculum: 1) Preparation of microbial slant seeds: inoculate mycelia or spores of actinomycete Streptomyces griseus (Streptomyces griseus) KJA04-02 on the slant of Gao's medium, and cultivate at 25-28°C for 7 Obtain seed slant days later. Gao's medium is 2 grams of starch, 0.05 grams of magnesium sulfate, 0.1 grams of potassium nitrate, 0.05 grams of sodium chloride, 0.05 grams of dipotassium hydrogen phosphate, 0.001 grams of ferrous sulfate, 2 grams of agar, 100 ml of water, pH7.2- 7.4.

[0017] The bacterium Bacillus subtilis (Bacillus subtilis) KJB04-11 was inoculated on the slant of PDA medium, and cultured at 28-37° C. for 1 day to obtain the seed slant.

[0018] The PDA medium is 20 grams of potatoes, 2 grams of glucose, 2 grams of agar, and 100 milliliters of water.

[0019] 2) Cultivation of microbial liquid seeds: transfer the slant seeds of the actinomycete Streptomyces griseus (Streptomyces griseus) KJA04-02 to the liquid...

Embodiment 3

[0027] Example 3 Preparation of microbial inoculum: 1) Preparation of microbial slant seeds: Inoculate mycelia or spores of actinomycete Streptomyces griseus (Streptomyces griseus) KJA04-02 on the slant of Gao's medium, and cultivate at 25-28°C for 7 Obtain seed slant days later. Gao's medium is 2 grams of starch, 0.05 grams of magnesium sulfate, 0.1 grams of potassium nitrate, 0.05 grams of sodium chloride, 0.05 grams of dipotassium hydrogen phosphate, 0.001 grams of ferrous sulfate, 2 grams of agar, 100 ml of water, pH7.2- 7.4.

[0028] The bacterium Bacillus subtilis (Bacillus subtilis) KJB04-11 was inoculated on the slant of PDA medium, and cultured at 28-37° C. for 1 day to obtain the seed slant.

[0029] The PDA medium is 20 grams of potatoes, 2 grams of glucose, 2 grams of agar, and 100 milliliters of water.

[0030] 2) Cultivation of microbial liquid seeds: the slanted seeds of the actinomycete Streptomyces griseus (Streptomyces griseus) KJA04-02 were transferred to ...

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PUM

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Abstract

The invention relates to the technical field of biological pesticides, in particular to a microbial agent as well as a preparation method and application thereof. The microbial agent comprises streptomycesgriseus and bacillussubtilis; the streptomycesgriseus KJA04-02 is preserved in China Center for Type Culture Collection on Sep. 28th, 2010, with the collection number of CCTCC M2010253; and the bacillussubtilis KJB04-11 is preserved in the China Center for Type Culture Collection on Sep. 28th, 2010, with the collection number of CCTCC M2010254. The microbial agent disclosed by the invention is efficient, non-toxic and environment-friendly, is simple in preparation process, has a good effect on controlling root knot nematodiasis of vegetables and fungal diseases, and can be used for biologically controlling the root knot nematodiasis of the vegetables and the fungal diseases.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to a microbial agent and its preparation method and application. Background technique [0002] The root-knot nematode disease is widespread in the world, and the annual crop loss caused by the root-knot nematode damage reaches more than 100 billion U.S. dollars, which can reduce the world's agricultural production by 24.5% on average every year. In my country, root-knot nematodes occur very seriously in many provinces and cities, and the crops that are damaged include vegetables, tobacco, flowers, cotton, soybeans, peanuts, wheat, rice, and traditional Chinese medicines. In recent years, root-knot nematode disease has been very serious in greenhouses and greenhouses in many areas of our country. The lightly damaged vegetables have a 20-30% reduction in yield, and the serious ones have reached more than 70% or even no harvest. Fungal diseases are also important disease...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20A01N63/02A01P3/00A01P5/00C12R1/125C12R1/545
Inventor 张惠文李旭苏振成王秀娟孙健张成刚
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI