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System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system

A flow cytometer and flow channel technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, instruments, etc., can solve problems such as raising prices

Inactive Publication Date: 2012-05-23
SONY CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When a 40-channel microfluidic sorting system can match the throughput of a single droplet-based cell sorting system, most potential users are unwilling (or unable) to pay 40 times more for additional components of the required system components
For example, it is easy to calculate that the typical price of the lasers, filters, photodetectors, and data acquisition components required for a single-cell sorting channel is $15,000, so manufacturing a 40-channel microfluidic sorting system would cost upwards of $600,000, and when retail , the manufacturer will raise the price in order to gain profits

Method used

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  • System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system
  • System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system
  • System and method for the measurement of multiple emissions from multiple parallel flow channels in a flow cytometry system

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Embodiment Construction

[0030] In order to enhance the understanding of the principles of the invention, reference will now be made to the embodiments illustrated in the drawings, and specific language will be used to describe the same features. It should be understood, however, that no limitation of the scope of the invention is thereby intended, but alternatives and further modifications of the illustrated apparatuses and methods and the principles of the invention illustrated are intended to be protected as commonly understood by those skilled in the art to which the invention pertains further application.

[0031] From the following description, those skilled in the art will realize that this embodiment can utilize any parallel channel system and is not only applicable to microfluidic systems, which is done only to describe the concept of the present invention in a convenient environment. It should also be understood that the term "parallel" as used herein is intended to include any number of flo...

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Abstract

A system and method for the measurement of multiple emissions in multiple flow channels in a flow cytometry system is disclosed where each excitation source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all sources in all flow channels, and the emissions are segregated using Fourier Transform techniques. The system and method are well-suited to microfluidic applications.

Description

[0001] Cross-References to Associated Applications [0002] This application claims priority to US Provisional Patent Application No. 61 / 222,509, filed July 2, 2009, which is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates generally to flow cytometry systems, and more particularly to systems and methods for measuring multiple emissions from multiple parallel flow channels in a flow cytometry system. Background technique [0004] Flow cytometry-based cell sorting was first introduced into the research world more than two decades ago. It is a technique that has been widely used in many fields, including life science research, and has become an important tool for those working in fields such as genetics, immunology, molecular biology and environmental science. Unlike bulk cell separation techniques such as immuno-panning and magnetic column separation, flow cytometry-based cell sorting equipment processes individual cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34
CPCG01N15/1429G01N15/1434G01N15/149C12M41/36G01N21/63
Inventor 加里·杜拉克
Owner SONY CORP