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Method for preparing high-purity madecassoside and asiaticoside B

A technology of madecassoside and madecassoside, which is applied in the field of preparation of high-purity madecassoside and madecassoside B, can solve the problems of inability to separate madecassoside and the like

Inactive Publication Date: 2012-05-30
SHANGHAI INST OF PHARMA IND CO LTD
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  • Abstract
  • Description
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Problems solved by technology

[0006] The purpose of the present invention is to solve the above-mentioned defects that madecassoside and its isomer volume madecassoside B cannot be separated under the existing HPLC conditions, and provide a simple and easy separation of high-purity madecassoside and its Method for isomerizing madecassoside B

Method used

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  • Method for preparing high-purity madecassoside and asiaticoside B

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Embodiment 1-12

[0016] 1. Preparation: (The sample sent is the raw material of Centella asiaticoside)

[0017] Separation of madecassoside from its isomeric volume madecassoside B by cyclodextrin additive method using preparative high-performance liquid chromatography. Shimadzu preparative high-performance liquid chromatography, including Shimadzu Class-VP workstation, LC-8A pump, controller of SCL-10Avp pump, SPD-M10Avp detector, FRC-10A automatic collector, mobile phase selection acetonitrile: 2mmol / L cyclodextrin aqueous solution (v / v)=26:74, chromatographic column For: SHIMADZU shim-pack PRC-ODS column (20mm×250mm), flow rate: 6ml / min, injection volume: 5ml, detection wavelength: 204nm, according to the chromatographic peaks were collected to obtain the collected solution of preparative liquid chromatography, the collected solution The solvent was recovered separately to obtain a white powder.

[0018] 2. Extraction:

[0019] Take 50 mg of the white powder obtained above, select n-butan...

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Abstract

The present invention relates to a method for preparing high-purity madecassoside and asiaticoside B. The method comprises performing preparative high-performance liquid chromatography by using a mobile phase added with cyclodextrin, collecting two eluates according to chromatographic peaks, respectively, recovering the solvent in the eluates, and extracting to remove cyclodextrin. By adopting preparative high-performance liquid chromatography and cyclodextrin-containing mobile phase, the invention has no need for a special analytical column, is cheap and feasible, and has substantial improvements and advantages compared with other methods.

Description

technical field [0001] The invention relates to the technical field of a purification method for madecassoside and its isomer madecassoside B. Background technique [0002] Centella asiatica (L.) Urban. is the dry whole herb of Centella asiatica of the Umbelliferae family Centella asiatica. It was first recorded in "Shen Nong's Materia Medica", and it tastes bitter and cold. Mainly severe fever, malignant sores, carbuncles, and body heat are listed as middle-grade. Centella asiatica is a commonly used traditional Chinese medicine recorded in the 2010 edition of "Chinese Pharmacopoeia". It has the effects of clearing away heat and dampness, detoxifying and reducing swelling. Meningitis, acute glomerulonephritis, etc. my country has a history of 2,000 years of using Centella asiatica for external and internal use to treat diseases. Many countries and regions such as South Africa, India, and Southeast Asia also use it as a traditional medicine. The main chemical components o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 邵燕陈云艳孔德云翁小香周靖欧阳丹薇吕峰高雯张静成亮
Owner SHANGHAI INST OF PHARMA IND CO LTD
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