Method For Analyzing Nucleic Acid Mutation Using Array Comparative Genomic Hybridization Technique

Abstract

The present invention provides a method for analyzing nucleic acid mutations using an array comparative genomic hybridization technique, which reduces false positives and false negatives and improves reliability of analysis results. The method for analyzing nucleic acid mutations using array comparative genomic hybridization technology includes: [step (a): making a plurality of labeled sample nucleic acids contact with a plurality of identical probe nucleic acid groups one by one to achieve hybridization], [step (b): obtaining the Labeling intensities F1 to Fn of sample nucleic acids in each of the spots hybridized to spots X1 to Xn, which have been hybridized with the same probe nucleic acid], [step (c): determining from the comparison of F1 and F2 Whether each of the n-1 comparison values ​​of the comparison values ​​of F1 and Fn falls within the specified numerical range], and [Step (d): comparing the comparison values ​​that have been determined not to fall within the specified numerical range Whether the number of the specified number exceeds the specified number and, in the case of exceeding the specified number, it is judged that the sample point X1 is positive]. n is an integer of 3 or more.

Description

technical field [0001] The present invention relates to a method for analyzing nucleic acid mutations using an array comparative genomic hybridization technique, which reduces the influence of false positives and the like from analysis results and improves the reliability of analysis results. Background technique [0002] Array comparative genomic hybridization (CGH) technology is known as a method for detecting genome structural abnormalities occurring at the level of several 10 kb to several Mb, such as genome copy number abnormalities, in a short period of time (see, for example, Non-Patent Document 1 and 2). [0003] In the case of diagnostic arrays for genetic diseases and the like using array CGH, it becomes necessary to accurately diagnose copy number abnormalities directly related to pathological conditions. Therefore, a nucleic acid as a standard must not have copy number variation (CNV) and thus careful selection and confirmation should be made to include rare CNV...

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Problems solved by technology

In the case where it is judged to be a false positive or a false negative, reexamination becomes necessary and a problem arises that a diagnosis cannot be made with one examination

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