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Two-dimensional chromatography multi-channel separating and purifying apparatus and method

A separation and purification, two-dimensional chromatography technology, applied in separation methods, chemical instruments and methods, measuring devices, etc., can solve the problems of large column packing consumption, less sample loading, long elution time, etc., and achieve high separation efficiency. , the effect of reducing consumption and low cost

Active Publication Date: 2012-06-13
哈尔滨工大中奥生物工程有限公司
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  • Abstract
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problems in the traditional chromatographic separation and purification technology, such as less sample loading, large consumption of column packing, long elution time, large amount of eluent and low monomeric target product for separation and purification, and provides a two Dimensional chromatography multi-channel separation and purification device and method

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  • Two-dimensional chromatography multi-channel separating and purifying apparatus and method
  • Two-dimensional chromatography multi-channel separating and purifying apparatus and method
  • Two-dimensional chromatography multi-channel separating and purifying apparatus and method

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specific Embodiment approach 1

[0026] Specific implementation mode one: the following combination figure 1 Describe this embodiment, the two-dimensional chromatographic multi-channel separation and purification device described in this embodiment includes a central distributor 1, a material tank 3, a mobile phase tank 4, a stationary phase tank 5, a pressure pump 6, a detector 7 and n Column 2,

[0027] The upper port of each chromatographic column 2 is connected with the central distributor 1 through a parallel valve, and the lower port of each chromatographic column 2 is provided with a discharge valve,

[0028] The lower port of the previous chromatographic column 2 also communicates with the upper port of the next chromatographic column 2 through a series tube, and a series valve is arranged on the series tube, so that n chromatographic columns 2 are connected end to end, and the last chromatographic column 2 The output of the lower port is sent to the detector 7 through the detection valve, and the lo...

specific Embodiment approach 2

[0039] Specific implementation mode two: the following combination figure 2 To illustrate this embodiment, the two-dimensional chromatographic multi-channel separation and purification method based on the two-dimensional chromatographic multi-channel separation and purification device described in the first embodiment, the method includes the following steps:

[0040] Step 1, making a plurality of chromatographic columns 2 in the two-dimensional chromatographic multi-channel separation and purification device in parallel mode, and filling the separation and purification medium in the stationary phase tank 5 into each chromatographic column 2 through a pressure pump 6;

[0041] Step 2, making the plurality of chromatographic columns 2 in the two-dimensional chromatographic multi-channel separation and purification device be in a series mode, and the material in the material tank 3 is adsorbed in the first and second chromatographic columns 2 by the pressure pump 6;

[0042] St...

specific Embodiment approach 3

[0045]Specific embodiment three: this embodiment will further explain embodiment two, and the valves in the open state in step one are: the parallel valve provided at the upper port of each chromatographic column 2, the discharge valve provided at the lower port of each chromatographic column 2 and A discharge valve arranged at the lower end of the stationary phase tank 5 .

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Abstract

The invention belongs to the field of chemical engineering, and provides an apparatus and a method of two-dimensional chromatography separating and purifying. According to traditional chromatography separating and purifying technologies, a fed sample amount is small, column filling material consumption is high, an elution time is long, eluant consumption is high, and the monomer target product yield of separating and purifying is low. The apparatus provided by the invention comprises a central distributor, a material tank, a mobile phase tank, an immobile phase tank, a pressure pump, a detector and n chromatographic columns. The method comprises steps that: step 1, a parallel connection mode is adopted, wherein a separating and purifying medium in the immobile phase tank is filled into every chromatographic column by using the pressure pump; step 2, a series connection mode is adopted, wherein a material in the material rank is absorbed in a first chromatographic column and a second chromatographic column by using the pressure pump; step 3, series connection elution is carried out, wherein the mobile phase in the mobile phase tank is circulated in the n chromatographic columns by using the pressure pump; step 4, parallel connection elution is carried out, wherein the mobile phase in the mobile phase tank flushes from top to bottom in every chromatographic column by using the pressure pump, eluant in each chromatographic column is collected from section to section, such that two-dimensional chromatography separation is finished.

Description

technical field [0001] The invention relates to a device for separation and purification of a chromatographic column and a method for separation and purification, belonging to the field of chemical industry. Background technique [0002] At present, almost all fields involve the application of chromatography and related technologies. The application of chromatography technology is becoming more and more common, and chromatography technology is playing an increasingly important role in scientific research and industrial production. The early invention of chromatography technology was mainly used in chemical analysis. As a method of physical and chemical separation and analysis, it can separate compounds with little difference in physical and chemical properties. In particular, the chemical or physical properties of the various components of the mixture are very close, and other separation techniques are difficult or impossible to apply, and chromatography technology has incre...

Claims

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Application Information

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IPC IPC(8): B01D15/18G01N30/46
Inventor 王振宇曾祥宏赵海田
Owner 哈尔滨工大中奥生物工程有限公司
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