Method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum

Abstract

The invention relates to a method for determining regeneration consumption in-vivo carbohydrate of roots of leaf-removed lolium multiflorum. The method comprises the following steps of: taking 36-48 plants of growth consistent lolium multiflorum in two weeks, after growing the lolium multiflorum for eight weeks under the conditions of the temperature of 18-25 DEG C and illumination of 6-8 h everyday, respectively detecting the lolium multiflorum to obtain the average carbon content of roots of the lolium multiflorum before removing leaves, the average carbon content of crops of the lolium multiflorum before removing leaves, and cutting off the roots to culture by keeping out of the sun, detecting the total carbon content of the regeneration consumption in-vivo carbohydrate of the roots of the lolium multiflorum after removing leaves each time, and calculating the amount of the regeneration consumption carbohydrate of the roots of regenerated lolium multiflorum. The method for determining the regeneration consumption in-vivo carbohydrate of the roots of the leaf-removed lolium multiflorum provided by the invention has the advantages of being simple in determination method, simple for operation and increased in production efficiency and working efficiency.

Description

technical field [0001] The invention relates to a measurement method in the technical field of forage grass, in particular to a measurement method for the consumption of carbohydrates in the body of ryegrass root system regeneration. Background technique [0002] After the ryegrass multiflora is eaten by herbivores or artificially cut, new leaves can grow on the grazing or clipped parts, or the leaves that have been partially damaged can continue to grow. "Regeneration" is of great significance for maintaining its own survival, resisting damage from herbivores and increasing grass production. [0003] A key problem for ryegrass regrowth is the source of carbohydrates needed for regrowth after defoliation. Because after ryegrass is grazed or cut, the leaves need carbon, nitrogen, phosphorus, water and many other substances to maintain growth. In the ryegrass leaves that have been dehydrated, the content of carbon is about 34-38%, the content of nitrogen is only about 2%-3%,...

AI Technical Summary

Problems solved by technology

During the regeneration process of ryegrass multiflora leaves after grazing or pruning, carbohydrates are consumed not only for the regeneration of leaves, but also for the growth of roots. Find out the carbohydrates stored in ryegrass multiflora before leaves are removed How many compounds will directly participate in the process of root growth will help to clarify the distribution pattern of carbohydrates stored in Lolium multiflora, which is of great significance for the study of regeneration of Lolium multiflora. So far, There is no related report on the method of determining the consumption of stored carbohydrates in Lolium multiflora during root regeneration