Production method for mycelium pellets of black fungus and products of mycelium pellets of black fungus
A technology of black fungus, mycelium ball, applied in the fields of botanical equipment and methods, fertilizer mixtures, gardening, etc.
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Embodiment 1
[0047] 1. Preparation of black fungus mycelium balls
[0048] 1.1 Activate and rejuvenate the preserved species of black fungus with potato medium (PDA medium), place them in a 26°C incubator and culture them statically for 7 days to obtain production slant species, and store them in the refrigerator for later use; the formula of the PDA medium is as follows: (g / L): Peeled potato 200, glucose 15, agar 20, add water to the required volume, pH natural;
[0049] 1.2 Expand the culture of the production slant seed with the shake flask medium to obtain the first-class species of the shake flask; the formula of the shake flask medium is ( / L): 50ml of hawthorn juice, 50ml of carrot juice, 5g of white sugar, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 2g, ZnSO 4 ·7H 2 O 0.1g, add water to make up to 1000 ml, pH is natural; culture conditions: 100mL / 500mL filling, insert 1cm 2 cultured on a slant surface with a rotary shaker at 250rpm for 5 days at a temperature of 30°C;
[0050] 1.3 Use 5%...
Embodiment 2
[0067] 1. Preparation of black fungus mycelium balls
[0068] 1.1 Activate and rejuvenate the preserved species of black fungus with potato medium (PDA medium), put them in a 28°C incubator and culture them statically for 7 days, and obtain the production slant species, which are stored in the refrigerator for later use; the formula of the PDA medium is as follows: (g / L): Peeled potato 250, glucose 15, agar 15, add water to the required volume, pH natural;
[0069] 1.2 Expand the cultivation of the production slant species with the shake flask medium to obtain the first-grade species of the production shake flask; the formula of the shake flask medium is ( / L): 100 ml of carrot juice, 50 ml of grape juice, and 5 g of white sugar 、KH 2 PO 4 2g, MgSO 4 ·7H 2 O 2g, ZnSO 4 ·7H 2 O 0.25g, add water to the volume of 1000ml, pH is natural; culture conditions: 250mL / 500mL filling, insert 1cm 2 slant strains, cultivated on a rotary shaker at 120rpm for 7 days, at a temperature...
Embodiment 3
[0087] 1. Preparation of black fungus mycelium balls
[0088] 1.1 Activate and rejuvenate the preserved species of black fungus with potato medium (PDA medium), place them in a 30°C incubator and culture them statically for 7 days to obtain production slant species, and store them in the refrigerator for later use; the formula of the PDA medium is as follows: (g / L): Peeled potato 150, glucose 20, agar 15, add water to the required volume, pH natural;
[0089] 1.2 Expand the culture of the production slant species with the shake flask medium to obtain the first-class species of the production shake flask; the formula of the shake flask medium is ( / L): 50 ml of apple juice, 10 g of white sugar, KH 2 PO 4 5g, MgSO 4 ·7H 2 O 5g, ZnSO 4 ·7H 2 O 0.5g, add water to the volume of 1000ml, pH is natural; culture conditions: 250mL / 500mL filling, insert 1cm 2 slant strains, cultivated on a rotary shaker at 70rpm for 10 days, at a temperature of 20°C;
[0090] 1.3 Use 10% of the ...
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