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Artificial propagation method for Ooencyrtus kuvanae

A technology of moth-eating wasps and artificial reproduction, which can be applied to animal husbandry and other directions, and can solve the problems of no large moth-eating wasps and no large moth-eating wasps.

Inactive Publication Date: 2012-07-11
HEBEI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has not been found in the wild that the large moth egg jumper can parasitize tussah silkworm eggs, and so far, there has been no report of using tussah silkworm eggs to reproduce the big moth egg jumper

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Obtaining of surrogate host eggs

[0020] Tussah silkworm eggs were used as an alternative host to reproduce the large moth egg jumper wasp. Female tussah silkworm chrysalis were purchased in the market. Pupae were stored at 0-5°C before use. When in use, the tussah chrysalis is placed under room temperature conditions of 25° C. to allow it to emerge as an adult moth. Then the female adult moth is dissected to obtain dissected eggs, the eggs are washed and dried, and abnormal eggs are removed to obtain normal eggs.

Embodiment 2

[0022] (a) After the tussah silkworm chrysalis eclosion, the cesarean eggs were taken, and frozen at -4°C for 15 days;

[0023] (b) thawing the laparotomy eggs after cryopreservation in an artificial climate box at 5°C for 2 days;

[0024] (c) Put the thawed laparotomy eggs into the inoculation box with the adult larvae larvae for inoculation, and the ratio of the number of laparotomy eggs to larvae larvae adults is 3:1 (100 larvae larvae adults Small bees inoculate 300 eggs), and feed the big moth eggs and jump small wasp adults with honey water with a mass concentration of 15%, and the inoculation time is 5 days;

[0025] (d) After the inoculation, take out the caesarean eggs and culture them at 22°C. After about 19 days, a large moth egg will emerge, and a maximum of 8 large moth eggs will emerge from one egg, and a minimum of 2 large moth eggs will emerge. For bees, an average of 5.2 large moth eggs and small bees emerge from each egg.

Embodiment 3

[0027] (a) After the tussah silkworm chrysalis eclosion, the cesarean eggs were taken, and frozen at -2°C for 60 days;

[0028] (b) thawing the laparotomy eggs after cryopreservation in an artificial climate box at 12°C for 1 day;

[0029] (c) Put the thawed laparotomy eggs into the inoculation box with the adult larvae larvae for inoculation, and the ratio of the number of laparotomy eggs to larvae larvae adults is 5:1 (100 larvae larvae adults Small bees inoculate 500 eggs), and feed the big moth eggs and jump small wasp adults with honey water with a mass concentration of 15%, and the inoculation time is 3 days;

[0030] (d) After the inoculation, take out the caesarean eggs and culture them at 26°C. After about 17 days, a large moth egg will emerge and a large moth egg will emerge. A maximum of 9 large moth eggs will emerge from one egg, and at least 3 large moth eggs will emerge. Bees, on average, 5.5 large moth eggs and small bees emerge from each egg.

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PUM

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Abstract

The invention discloses an artificial propagation method for Ooencyrtus kuvanae. A caesarean egg of a tussah is used as a substitute host, the treated caesarean egg is used for inoculating Ooencyrtus kuvanae, and by culturing at normal temperature, a descendant adult fly with properties the same as that of the fly cultured from an original host egg can be obtained. By the method, Ooencyrtus kuvanae can be propagated indoors on large scale, and a practical technical means for biological control of gypsymoth in forests is provided.

Description

technical field [0001] The invention relates to the field of insect reproduction, in particular to an artificial reproduction method for the large moth egg jumping wasp. Background technique [0002] Ooencyrtus kuwanai Howard (Ooencyrtus kuwanai Howard, Hymenoptera: Ooencyrtidae) mainly parasitizes the eggs of Lymantria dispar Linnaeus, and also the scales of pine caterpillars (Dendrolimus spp.) and willow moths (Stilpnotia salicis Linnaeus). A parasitic wasp of the eggs of a pest of the order Pteroptera. It is known to be distributed in Jilin, Liaoning, Beijing, Hunan, Sichuan and other provinces in China, and it is distributed in Japan abroad. It has been introduced to many countries in Western Europe, North Africa and North America to control gypsy moths. [0003] Gypsy moths have one generation per year all over the world, and the egg stage is from August to April of the following year. The large moth egg jumping wasp can parasitize any developmental stage of the gypsy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 魏建荣王建军
Owner HEBEI UNIVERSITY
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