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Novel application of ricin B chain protein in immunoloregulation

A technology of ricin and immunomodulators, which is applied in the field of medicine and can solve the problem that the RTB chain has no immune activity

Inactive Publication Date: 2012-07-11
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RTB chains are generally considered to be nonimmunely active

Method used

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  • Novel application of ricin B chain protein in immunoloregulation
  • Novel application of ricin B chain protein in immunoloregulation
  • Novel application of ricin B chain protein in immunoloregulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Effect of RTB chain protein on NO secretion of peritoneal macrophages

[0016] Reagents and medicines: RTB chain protein, self-made, dissolved in normal saline, diluted to different concentrations (the same below), NO detection kit was purchased from Biyuntian Biotechnology Research Institute.

[0017] Animals: Balb / c mice were purchased from the Laboratory Animal Laboratory of Changchun Institute of Biological Products.

[0018] Methods: NO detection kit was used to detect the secretion of NO in mouse peritoneal macrophages. Mice were sacrificed, intraperitoneally injected with 5 mPBS, the abdomen of the mice was lightly rubbed, the abdomen was cut open, and the peritoneal cavity was repeatedly washed 2 or 3 times; the prepared macrophages were taken and the number of cells was adjusted to 1×10 7 cells / ml; Inoculate macrophages into 96-well culture plates, add different concentrations of RTB catalin 100 μl / well, collect supernatant after 24 hours, and use N...

Embodiment 2

[0020] Example 2: Effect of RTB chain protein on macrophage cytotoxicity

[0021] Reagents and medicines: RTB catenin, self-made, RPMI-1640 medium was purchased from GIBCO company.

[0022] Animals: Balb / c mice were purchased from the Laboratory Animal Laboratory of Changchun Institute of Biological Products.

[0023] METHODS: MTT assay was used to detect the cytotoxic effect of mouse peritoneal macrophages. The mice were killed, 5ml PBS was injected into the intraperitoneal cavity, the abdomen of the mice was lightly rubbed, the abdomen was cut open, and the peritoneal cavity was repeatedly washed 2 or 3 times; the prepared macrophages were taken, and the number of cells was adjusted to 1×10 7 cells / m; 0.1ml per well, inoculated macrophages into 96-well culture plates, added different concentrations of RTB catalin, cultured in 37°C, 5% CO2 incubator for 48 hours, added B16 target cells (the effect-to-target ratio was 10:1) ) for 48 hours; before terminating the culture, add...

Embodiment 3

[0026] Example 3: Effect of RTB chain protein on the proliferation of mouse spleen lymphocytes in vitro

[0027] Reagents and medicines: lipopolysaccharide and ConA were purchased from Sigma, and MTT was purchased from Sigma.

[0028] Animals: Balb / c mice were purchased from the Laboratory Animal Laboratory of Changchun Institute of Biological Products.

[0029] method:

[0030] 3.1 MTT method was used to detect the effect of RTB chain protein on the proliferation of unactivated mouse spleen lymphocytes. Aseptically prepare mouse spleen lymphocyte suspension, adjust the cell concentration to 1×10 7 cells / ml, add 100 μl per well of 96-well cell plate, then add 100 μl of different concentrations of RTB chain protein, culture for 44 hours, collect the supernatant for later use, add 10 μl MTT to each well, continue to incubate for 4 hours, add 100 μl DMSO to each well, shake for 10 minutes , Measure the absorbance value (A value) at a wavelength of 540nm. Calculate the value-a...

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Abstract

The invention relates to a novel application of ricin B chain protein in the aspect of immunoloregulation, which belongs to the scope of medicine field. An application of ricin B chain protein in the preparation of immunoregulators, and an application of ricin B chain protein in the preparation of agents for tumor adjuvant therapy are provided. Ricin B chain protein can activate mouse peritoneal macrophages, promotes mouse spleen T and B lymphocyte proliferation, improve NK cell activity, has the effect of immunoloregulation, can be used as an immunoregulator, and can be used in tumor adjuvant therapy.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the new application of ricin B chain protein in immune regulation. Background technique [0002] Ricin toxin (RT) is derived from castor bean glycoprotein with a molecular weight of 64kDa. The holotoxin of ricin consists of two polypeptide chains A and B linked together by a disulfide bond. RTA is a ribosome inactivating protein that can inhibit protein synthesis in mammalian cells; RTB chain is non-toxic and has lectin activity, which can bind to the receptors of galactose or mannose on the cell surface and assist the A chain to enter the cell perform biological functions. RTB is a molecule with a bilobal or dumbbell-shaped structure, consisting of two different spherical regions with the same folding topology, and each region includes three subregions (α, β, γ), only 1α and 2β have obvious galactose-binding activity. When both globular regions are mutated, RTB loses ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P35/00A61P37/02
Inventor 刘文森许娜侯峰徐静钱军万家余刘林娜孙玉成
Owner MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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