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Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction

A cell culture, combined loading technology, applied in the field of cell engineering and tissue engineering, can solve the problem of inability to simulate, and achieve the effect of preventing bacteria from entering

Inactive Publication Date: 2013-08-14
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the technical problem that the existing method cannot simulate the physical microenvironment where the cells in the body are in the shear force and the electric stimulation coexist, a kind of cell culture device with shear force and electric stimulation combined loading in parallel directions is proposed, and the shear force is adopted. Stimulation and electrical stimulation combined loading, placing cells under parallel loading of shear force stimulation and electrical stimulation, simulating the living environment of cells in vivo, promoting cell growth and differentiation, and constructing cells or tissues with specific functions

Method used

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  • Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction
  • Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction
  • Cell culture device capable of loading shear force stimulation and electrical stimulation jointly in parallel direction

Examples

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Effect test

Embodiment 1

[0050] Under sterile conditions, the cardiomyocytes of 1-3 day old SD rat suckling mice were divided into 1×10 5 / cm 2 The density was seeded on a glass slide 704 covered with collagen. After pre-cultivation for 3 days, the glass slide 704 seeded with cardiomyocytes was placed on the base 7012 between the two liquid storage tanks 7011 in the cell culture device 7. Two silica gel gaskets 703 are placed on the 704, and the two ends of the silica gel gaskets 703 are in close contact with the corresponding liquid storage tank bottom opening 705 respectively; the flat plate 702 is placed on the silica gel gasket 703, and the flat plate 702 is provided with screw holes, through which the bolts 709 Fix the plate 702 on the base 7012 .

[0051] The tank body cover 706 of the cell culture chamber 7 is fixed on the tank body 701 by bolts 709 . The upstream liquid storage bottle 3 and the downstream liquid storage bottle 4 are connected by three silicone tubes, such as figure 1 shown....

Embodiment 2

[0055] Under sterile conditions, the human umbilical vein endothelial cells were divided into 1 × 10 5 / cm 2 Density seeding on a glass slide 704. After 12 hours of pre-cultivation, the glass slide 704 inoculated with human umbilical vein endothelial cells is placed on the base 7012 between two liquid storage tanks 7011 in the cell culture device 7, on the glass slide 704 Place two silica gel gaskets 703, the two ends of the silica gel gasket 703 are in close contact with the corresponding bottom opening 705 of the liquid storage tank; the plate 702 is placed on the silica gel gasket 703, and the plate 702 is provided with screw holes, and the plate is fixed by bolts 709. 702 is fixed on the base 7012.

[0056] The tank body cover 706 of the cell culture chamber 7 is fixed on the tank body 701 by bolts 709 . The upstream liquid storage bottle 3 and the downstream liquid storage bottle 4 pass through three silicone tubes such as figure 1 connected as shown. Then the upstrea...

Embodiment 3

[0060] Under sterile conditions, the osteoblasts were divided into 1 × 10 5 / cm 2 The density was seeded on the silica gel membrane 704, and after 24 hours of pre-cultivation, the silica gel membrane 704 seeded with osteoblasts was placed on the base 7012 between the two reservoirs 7011 in the cell culture device 7, and placed on the silica gel membrane 704 Two silica gel gaskets 703, the two ends of the silica gel gasket 703 are in close contact with the corresponding bottom opening 705 of the liquid storage tank; the plate 702 is placed on the silica gel gasket 703, and the plate 702 is provided with screw holes, and the plate 702 is fixed by bolts 709. Fixed on the base 7012.

[0061] The tank body cover 706 of the cell culture chamber 7 is fixed on the tank body 701 by bolts 709 . The upstream liquid storage bottle 3 and the downstream liquid storage bottle 4 pass through three silicone tubes such as figure 1 connected as shown. Then the upstream liquid storage bottles 3...

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Abstract

The invention discloses a cell culture device capable of loading shear force stimulation and electrical stimulation jointly in the parallel direction. The cell culture device comprises a cell culture device, a peristaltic pump, a signal generator, an upstream liquid storage cylinder and a downstream liquid storage cylinder, wherein the cell culture device consists of a tank body, a flat plate, a silica gel gasket, a cell culture substrate, a cover and a bolt; the peristaltic pump, the upstream liquid storage cylinder, the downstream liquid storage cylinder and the like are connected by a silicone tube to form a culture solution perfusion system; a circular cell culture solution passes through the cell culture device, so that the flowing shear force stimulation is loaded to cells; and the signal generator is connected with a metal electrode on the cover of the tank body of the cell culture device, so that the uniform electrical stimulation of which the direction is parallel to that of shear force is loaded to the cells. By the cell culture device, the defect that the simulation of in-vivo cells in microenvironment in which the shear force stimulation and the electrical stimulation exist simultaneously cannot be performed by the conventional method is overcome, cell culture environment in which the shear force stimulation and the electrical stimulation are loaded jointly in the parallel direction is established, and the development of relevant tissue engineering reactors is expanded.

Description

technical field [0001] The invention relates to a cell culture device combined with parallel loading of shear force and electrical stimulation, belonging to the technical fields of cell engineering and tissue engineering. Background technique [0002] In tissue engineering, the in vitro functional culture of cells and tissues has become the core of tissue engineering and the necessary technical basis for the formation of tissue engineering industry. However, providing an in vitro growth environment similar to the living conditions of cells in vivo is crucial for the three-dimensional culture and functionalization of cells and tissues. Among them, physical influence factors such as mechanical force and electric field play a role that cannot be ignored. [0003] The mechanical environment has an important impact on life activities at all levels of organs, tissues, cells and even sub-cells. The fluid shear force of the interstitial fluid helps bone cells perceive changes in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/42
CPCC12M29/02C12M29/10C12M35/02
Inventor 樊瑜波李萍王梦航贾璐刘成国宋彦辉
Owner BEIHANG UNIV