Solution gradient dilution device with microfluidic chip

Abstract

The invention belongs to the field of chemical analysis, and in particular relates to a solution gradient dilution device with a microfluidic chip. The device consists of the microfluidic chip with a structure comprising an upper layer and a lower layer, wherein the upper layer of the microfluidic chip is a disk-shaped substrate with a radial micro-channel network; the lower layer of the microfluidic chip is a flat substrate; the disk-shaped substrate is packaged with the flat substrate relatively to form a micro-channel network on a bottom layer; the micro-channel network is provided with N concentric circular channels, N stages of zigzag channels and M holes used as inlets; and the last stage of zigzag channel is a linear channel. According to the solution gradient dilution device, fluid is distributed averagely by using the symmetric micro-channel network; complete mixing based on molecule diffusion is realized in the zigzag channels; and through a repeated dividing-mixing process, an initial solution is diluted into a solution with gradient distributed concentration. According to the dilution device, the using amount of test samples is small, the space is saved, and series concentration is generated quickly and stably.

Description

technical field [0001] The invention belongs to the field of chemical analysis, and in particular relates to a solution gradient dilution device for a microfluidic chip. Background technique [0002] In chemical and biochemical experiments, gradient dilution is one of the most commonly used operations, especially in cytotoxicity analysis, gradient dilution operation is more important, in drug dose response experiments, it is necessary to prepare a series of linear dilution or logarithmic dilution The drug concentration generally needs to span 3-6 orders of magnitude. The conventional gradient dilution method requires precise metering and mixing of solutions. The operation is time-consuming and laborious, and it is easy to cause experimental errors and affect the accuracy of experimental results. [0003] The emergence of microfluidic technology provides a powerful means for gradient dilution operation. In the channel of the microfluidic chip, the two solutions can form a co...

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Problems solved by technology

In the channel of the microfluidic chip, the two solutions can form a concentration gradient in the main channel after confluence by repeating the flow-mixing-splitting process (Jeon N L, Dertinger S K W, Chiu D T, Choi I ​​S, Stroock A D, Whitesides G M.Langmuir, 2000, 16, 8311-8316.), if the above solutions do not merge into one main channel in the last step, but disperse into multiple branch channels, a series of gradient dilution solutions are formed (Ye N N, Qin J H, Shi W W, Liu X, Lin B C.Lab Chip, 2007, 7, 1696-1704), this gradient dilution method can easily dilute a drug solution into a series of concentrations, and can be integrated with cell culture devices in Together, it is used for drug screening research, but if this method is to form a wider concentration range, the chip area is required to be significantly increased, and it is difficult to realize the combined dilution solution of multiple drugs, and the operation process becomes more complicated.

Through different flow resistance ratios, the gradient dilution operation of the solution can also be realized on the microfluidic chip, and a gradient with a concentration range spanning 6 orders of magnitude can be generated (Sugiura S, Hattori K, Kanamori T.Anal.Chem., 2010, 82,8278-8282), but this gradient dilution method also has the problem that increasing the concentration gradient branch channel will significantly increase the chip area, it is difficult to perform combined dilution of different solutions, and the concentration gradient distribution is not fine enough

The existing microfluidic gradient dilution method that has been reported so far, because the concentration distribution range of the diluted solution is not wide enough, it is difficult to dilute the combination of different substances, and cannot meet the needs of large-scale dilution of one solution and combined dilution of multiple solutions. There are many limitations in practical application

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