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Rhodococcus sp.RHA-MLDS with mlds gene knocked out and application thereof

A cgmccno.4379, gene technology, applied in the field of Rhodococcus sp.RHA-MLDS with MLDS gene knockout and its application, can solve the problem of lipid droplet binding protein blank and so on

Active Publication Date: 2015-11-25
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although lipid droplet binding proteins in eukaryotic cells have been mostly identified [4-7], bacterial lipid droplet binding proteins are still blank

Method used

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  • Rhodococcus sp.RHA-MLDS with mlds gene knocked out and application thereof
  • Rhodococcus sp.RHA-MLDS with mlds gene knocked out and application thereof
  • Rhodococcus sp.RHA-MLDS with mlds gene knocked out and application thereof

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Experimental program
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Embodiment 1

[0025] Experimental materials and equipment

[0026] 1. Strains and plasmids

[0027] Rhodococcus sp.RHA1 was donated by Professor Lindsay D.Eltis of the University of British Columbia (University of British Columbia) (it was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on December 8, 2010, and its address is People's Republic of China No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, classified as Rhodococcus sp., and the preservation number is CGMCCNo.4423; Escherichia coli S17-1 (ATCC47055) and pK18mobsacB (ATCC87097) integrated plasmid Purchased from ATCC, U.S.; pJAM2-eGFP expression plasmid was Daniel from the University of Munster (University of Munster), Germany Gifted by the professor, the Escherichia coli pJAM2-egfp / S17-1 containing the pJAM2-eGFP expression plasmid was deposited in the General Microorganism Center (CGMCC) of the...

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Abstract

The invention relates to an oil producing rhodococcus sp.RHA-MLDS having MLDS (Multiple Low Dose Streptozotocin) gene knocked out and a purpose of the oil producing rhodococcus sp.RHA-MLDS. Particularly, the invention relates to the oil producing rhodococcus sp.RHA-MLDS having MLDS gene knocked out, the purpose of the oil producing rhodococcus sp.RHA-MLDS, the application of the MLDS gene and / or an MLDS protein inhibitor in biodiesel production, and a purpose of the MLDS gene and / or the MLDS protein as well as a composition, which includes a recombinant MLDS gene protein expression product and the MLDS gene or includes MLDS gene recombinant expression carrier, in the preparation of medicines for preventing and / or treating metabolic diseases related to lipid metabolism and storage disorder. According to the invention, a high efficient microbe strain is provided for the production of the biodiesel. Meanwhile, according to the invention, a candidate medicine is provided for treating the metabolic diseases related to lipid metabolism and storage disorder.

Description

technical field [0001] The present invention relates to Rhodococcus sp. RHA-MLDS knocking out MLDS gene and use thereof, and relates to the application of inhibitors of MLDS gene and / or MLDS protein in the production of biodiesel, MLDS gene and / or MLDS protein and containing Use of a recombinant MLDS gene protein expression product, MLDS gene or a composition of a recombinant expression vector comprising the MLDS gene in the preparation of a medicament for preventing and / or treating metabolic diseases related to lipid metabolism and storage disorders. Background technique [0002] The shortage of energy and environmental pollution have severely restricted the development of the world economy and the improvement of living standards. Therefore, it is imperative to develop new energy sources, especially sustainable and renewable clean bioenergy. From the fact that my country's investment in green and clean energy in 2009 (US$34.6 billion) was more than a quarter of the world's...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/64A61K38/16A61K48/00A61P3/04A61P3/06A61P1/16A61P9/10C12R1/01
CPCY02E50/13Y02E50/10
Inventor 刘平生丁云峰张淑妍张慧娜汪洋杜雅兰于进海彭恭杨丽
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES