Radish cytoplasmic male sterility molecular markers and method for assisting selective breeding using same

A technology of cytoplasmic male sterility and assisted selection, which is applied in biochemical equipment and methods, microbe measurement/testing, DNA/RNA fragments, etc., and can solve the problems of slow breeding process, time-consuming and laborious, long cycle, etc.

Inactive Publication Date: 2012-07-18
NORTHWEST A & F UNIV
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Problems solved by technology

Due to sterility, the traditional breeding method needs to wait until the flowering stage is completed through direct observation in the field. For the excellent materials carrying the restorer gene, in order to transfer them into a ste

Method used

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  • Radish cytoplasmic male sterility molecular markers and method for assisting selective breeding using same
  • Radish cytoplasmic male sterility molecular markers and method for assisting selective breeding using same
  • Radish cytoplasmic male sterility molecular markers and method for assisting selective breeding using same

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Embodiment Construction

[0055] In this example, the cytoplasmic male sterile material of Baiyuchun radish was taken as the research object, of which 3 copies of BC1 generation: B20×A20, B21×A21, B30×A30 and 4 copies of F2 generation: A20-1, A20-2, A21- 2, A30. The B20×A20 population was used as marker screening material.

[0056] The study used RAPD technology to screen the specific markers related to radish cytoplasmic male sterility, further cloned and sequenced the specific fragments, and converted them into SCAR markers, and calculated the linkage distance of the markers based on the segregation performance of the markers in the verification population. and SACR markers for molecular breeding.

[0057] First, analyze the genetic law of the sterile material through the fertility performance, then screen the segregated population with RAPD primers, clone and sequence the specific fragment and convert it into a SCAR marker, and then calculate its genetic distance in the population to verify its rel...

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Abstract

The invention discloses radish cytoplasmic male sterility molecular markers and a method for assisting selective breeding using same. The method comprises the steps of: based on genome DNA (Deoxyribonucleic Acid) of fertility separated F2 generation and first backcross generation of white radish cytoplasmic sterility material as templates, carrying out RAPD (Random Amplified Polymorphic DNA) analysis and multiple repeated tests to screen to obtain two RAPD markers, namely S88-1526 and S1344-415, linked with the radish cytoplasmic sterility fertility restorer gene, wherein the S88-1526 and S1344-415 markers are respectively located at both sides of the restorer gene and have the genetic distances of 4.2cm and 6.9cm, respectively. Furthermore, the RAPD marker S88-1526 is successfully transformed into SCAR (Sequence Characterized Amplified Region) marker scar-88. Linkage analysis shows that the cross-over value between the marker scar-88 and the restorer gene is 4.28%, and the corresponding genetic distance is 4.24cm. The radish cytoplasmic male sterility molecular markers can be used for performing assisted selection of early molecular markers during radish cytoplasmic male sterility breeding, so as to accelerate the breeding progress.

Description

technical field [0001] The invention belongs to the fields of agricultural vegetable variety selection and biotechnology, and in particular relates to RAPD and SCAR markers of radish cytoplasmic male sterility and their application for auxiliary selection breeding. Background technique [0002] For more than 30 years, plant male sterility has been a hot field in crop genetics, breeding and biological research. Cytoplasmic male sterility (cytoplasmic male sterility, CMS) is one of the most useful types of male sterility. In the 1970s, Ogura reported that cytoplasmic male sterility (CMS) in radish was jointly controlled by cytoplasmic sterile gene S and two pairs of nuclear sterile genes ms. Since the discovery of Ogura sterile cytoplasm, scholars at home and abroad have conducted various researches on the sterility mechanism and restoration genes of radish male sterility. slow. RAPD (Random Amplified Polymorphic DNA) was proposed by two research groups of Williams and Wels...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 张鲁刚郑晨光于娜娜许小勇惠麦侠牛娜
Owner NORTHWEST A & F UNIV
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