Method and kit for detecting susceptibility gene of colorectal cancer

Abstract

The invention relates to the field of gene engineering and provides a method and a kit for detecting a susceptibility gene of colorectal cancer. The method for detecting the susceptibility gene of the colorectal cancer comprises the following steps: A, amplifying a plurality of object regions in samples to be detected by using a colorectal cancer susceptibility gene specific primer, and constructing a sequencing library on the basis of an amplified product; B, carrying out single molecule amplification on the sequencing library to obtain a plurality of single molecule amplified products corresponding to the target regions; and C, simultaneously carrying out high throughout gene sequencing on the single molecule amplified products to obtain the sequence information of the object regions. According to the detection method and the kit provided by the invention, the regions of the colorectal cancer susceptibility gene are simultaneously sequenced by using a high throughout sequencing technology, so that the detection efficiency is increased and the detection cost is reduced; and meanwhile, the accuracy and the sensitivity of detection are improved.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a method and kit for detecting colorectal cancer susceptibility genes. Background technique [0002] Susceptibility genes refer to genes or alleles that have a certain association with a specific disease. Detect the susceptibility genes and mutation sites of complex diseases to obtain their specific gene sequence information, and then combine further molecular biology tests, clinical trials, clinical observations and statistical analysis of comprehensive data to establish disease models, which can realize Assess the susceptibility to disease early and take corresponding preventive measures to reduce the probability of disease occurrence. A large number of studies have confirmed that genetic factors play an important role in the susceptibility of diseases. The genetic factors of complex diseases have confirmed the existence of minor gene dosage effects, that is, the accumulatio...

AI Technical Summary

Problems solved by technology

Currently, the most common method for detecting colorectal cancer susceptibility genes is Sanger sequencing, which can detect colorectal cancer susceptibility genes regionally, but Sanger sequencing can only sequence a certain region of a sample at a time , so the detection efficiency is low and the detection cost is high; and due to the limitation of the sanger sequencing principle, double peaks or even multiple peaks will appear when detecting the signal of a sample with a heterozygote, making the sample unable to be accurately identified, resulting in low detection sensitivity (20%)

However, the detection kits based on this method currently on the market have the same defects.

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