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Heavy metal control method for haematococcus pluvialis culture process

A technology for Haematococcus pluvialis and Haematococcus pluvialis powder is applied in the field of bioengineering, can solve problems such as undiscovered technical reports, and achieve the effects of no side effects, easy operation and simple method

Active Publication Date: 2012-07-18
云南绿A生物产业园有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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  • Heavy metal control method for haematococcus pluvialis culture process
  • Heavy metal control method for haematococcus pluvialis culture process
  • Heavy metal control method for haematococcus pluvialis culture process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0031] Embodiment 2 determines the type and concentration of cleaning agent

[0032] Table 2 Test results of heavy metals and astaxanthin content in the algae powder of Haematococcus pluvialis with different cleaning agents under different operating conditions

[0033]

[0034] *Not treated with detergent.

[0035] According to the test setup and test results, it can be seen that:

[0036] 1. Using CH 3 After cleaning the mud of Haematococcus pluvialis with COOH, no significant reduction of Pb and Cd content was found.

[0037] 2. Using HCl, HNO 3 After washing the Haematococcus pluvialis mud, the content of Pb and Cd decreased significantly when the volume ratio was 1%. Compared with the control, the content of Pb decreased by 87.22% and 87.09% respectively; the content of Cd decreased by 90.91%.

[0038] 3. Suitable cleaning agents are HCl, HNO 3 ; The suitable cleaning agent concentration is 1% by volume.

Embodiment 3

[0039] Embodiment 3 determines the ratio of suitable Haematococcus spores (algae mud) and cleaning agent and when cleaning between

[0040] Table 3 The test results of heavy metals and astaxanthin in the algae powder of Haematococcus pluvialis with the ratio of Haematococcus spores (algae mud) to cleaning agent (mass volume ratio) of 1:4 at different cleaning times

[0041]

[0042] *Not treated with detergent.

[0043] Table 4 cleaning time is under 12h, the test result of heavy metal and astaxanthin content in the algal powder of Haematococcus pluvialis of different Haematococcus spores (algae mud) and cleaning agent ratio (mass volume ratio)

[0044]

[0045]

[0046] *Not treated with detergent.

[0047] According to the above test results, it can be seen (same as above):

[0048] 1. The cleaning time is more than 4 hours, the effect is better; the cleaning time is 8h, 12h and 15h, and the difference of Pb and Cd content is not obvious;

[0049] 2. The effe...

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Abstract

The invention provides a heavy metal control method for the haematococcus pluvialis culture process. The method includes the following steps: (1) according to the mass-to-volume ratio of 1:1 to 1:8, haematococcus pluvialis mud and cleaning agent are mixed into mixture; (2) the mixture obtained in step 1 is put on a shaker and washed for 1 to 15 hours; (3) the mixture washed in step 2 is centrifuged, supernatant is removed, and alga mud is collected; (4) the alga mud collected in step 3 is then washed by water for 2 to 3 times; (5) the alga mud washed in step 4 is centrifuged, and alga mud is collected; (6) the alga mud collected in step 5 is then dried and ground, and the Pb and Cd contents in the alga mud are tested. The method is applied in the production of haematococcus pluvialis powder, and can control the Pb content in haematococcus pluvialis powder at less than 2.0mg / Kg and the Cd content at less than 0.1mg / Kg; a spectrophotometric test shows that the content of astaxanthin is not notably affected, and an HPLC (High Performance Liquid Chromatography) test shows that the cis-astaxanthin structure and the trans-astaxanthin structure are not notably changed.

Description

technical field [0001] The invention relates to a method for controlling heavy metals, in particular to a method for controlling heavy metals in the cultivation process of Haematococcus pluvialis, and belongs to the technical field of bioengineering. Background technique [0002] Haematococcus pluvialis belongs to the division Chiorophata, the class Chiorophyceae, the order Volvocales, the family Haematococcaceae, and the genus Haematococcus. In a specific ecological environment, it can form chlamydospores and accumulate a large amount of astaxanthin. It is the organism most capable of synthesizing and accumulating astaxanthin in a large amount in nature, and it is also the organism with the highest natural astaxanthin content in nature. Astaxanthin is a biological antioxidant, which has a strong ability to remove free radicals, protect cells and tissues from lipid oxidation and peroxidation damage, and has broad applications in human functional food, medicine and precious a...

Claims

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Application Information

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IPC IPC(8): C12Q3/00
Inventor 陈建荣杜伟春鸭乔张瑶
Owner 云南绿A生物产业园有限公司
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