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Purification method and application of water-soluble alkaloid with alpha-glycosidase activity inhibition function in mulberry branch bark

A water-soluble and glycosidase technology, which is applied to medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, etc., can solve the problems of not mentioning the effective part and the content of the active ingredient, and achieve saving in dosage and price The effect of cheap, good comprehensive utilization prospects

Inactive Publication Date: 2012-07-25
NANKAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Patent application No. 200810021592.X describes an extract from mulberry bark that has a hypoglycemic effect, but does not mention the effective part, let alone the content of the active ingredient

Method used

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  • Purification method and application of water-soluble alkaloid with alpha-glycosidase activity inhibition function in mulberry branch bark
  • Purification method and application of water-soluble alkaloid with alpha-glycosidase activity inhibition function in mulberry branch bark
  • Purification method and application of water-soluble alkaloid with alpha-glycosidase activity inhibition function in mulberry branch bark

Examples

Experimental program
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preparation example Construction

[0022] 1. Preparation and application of total extract of water-soluble alkaloids from mulberry bark

[0023] Firstly, the mulberry branch bark is crushed, and the extraction solvent is added (water: alcohol=100%-20%:0%-80% as the extraction solvent, adjust the PH2-7 of the extraction solution), and the volume of the extraction solvent is the weight of the mulberry branch bark (Kg) 6-10 times as much as; heat reflux, filter, repeat extraction 2 times, and combine the filtrate of 2 times;

[0024] The extract is concentrated to 1 / 2 to 1 times the weight of the original mulberry branch bark;

[0025] Add methanol or ethanol with a volume percentage of 75% to 100%, the added amount is 3 to 5 times the volume of the concentrated extract obtained in step 2, and perform alcohol precipitation at 4 to 30°C; centrifuge or filter to remove the residue And insoluble matter to obtain the supernatant;

[0026] Take the supernatant after alcohol precipitation and concentration, purify it with a ca...

Embodiment 1

[0029] Add 1Kg of crushed mulberry bark, add 10L of water, adjust pH2, heat to reflux for 2 hours, and filter; repeat the extraction twice, combine the filtrate twice, concentrate to 500mL, add 1500mL of 95% ethanol, let stand overnight at room temperature, filter to remove insoluble The filtrate is applied to Zerolit 225 cationic resin, washed with deionized water to remove non-adsorbed impurities, then eluted with 1 mol ammonia water, and the eluent is applied to D380 anionic resin, collect the non-adsorbed part, adjust pH 8, pass through the membrane, and dry the filtrate to obtain Light yellow water-soluble total alkaloid extract 1.3g, DNJ-content 62%.

Embodiment 2

[0031] Add 1Kg of crushed mulberry bark, add 8L of 80% methanol, adjust the pH to 7, heat to reflux for 2 hours, and filter; repeat the extraction twice, combine the filtrate twice, concentrate to 500mL, add 1500mL of 95% ethanol, let stand overnight at room temperature, and filter , Remove the insoluble matter, put 0017 cationic resin on the filtrate, wash with deionized water to remove non-adsorbed impurities, and then eluted with 0.5mol ammonia water, and put D261 anionic resin on the eluent, collect the non-adsorbed part, adjust pH 8, filter with membrane, filtrate After drying, 11 g of light yellow water-soluble total alkaloid extract with a DNJ content of 69% is obtained.

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PUM

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Abstract

The invention provides an extraction and purification method of water-soluble total alkaloid with an alpha-glycosidase activity inhibition function from mulberry branch bark and application of the water-soluble total alkaloid. The purification method comprises the following steps: based on the mulberry branch bark as a raw material, adding an extraction solvent (water and ethanol at a ratio of 100-20 percent to 0-80 percent), extracting, heating and refluxing, filtering, repeatedly extracting twice, combining two filtrates, and concentrating; adding 75-100 percent methanol or ethanol by volume, and performing alcohol precipitation at a temperature of between 4 and 30 DEG C; centrifuging or filtering to remove residues and insoluble substances, collecting the supernatant after alcohol precipitation and concentration, purifying through a cation exchange resin column, washing non-adsorbed impurities with deionized water, and eluting with 0.2 to 2mol of ammonia water; passing the eluate through an anoin exchange resin, washing with deionized water, collecting the non-adsorbed part, and regulating the pH to 8; filtering with a membrane, drying the filtrate to prepare powder, namely light-yellow water-soluble total alkaloid extract, wherein the extract can inhibit the activity of alpha-glycosidase, and the content of DNJ (1-deoxynojirimycin) is between 50 and 70 percent.

Description

Technical field [0001] The invention relates to a method for separating and purifying water-soluble alkaloids in mulberry branch bark with the effect of lowering blood sugar and its application. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia caused by insufficient insulin secretion and / or impaired insulin action. It is manifested as an abnormally high blood and urine glucose concentration. When blood sugar and urine sugar are too high, the typical "three more and one less" symptoms may appear, that is, polydipsia, polyuria, polyphagia, weight loss, and fatigue. Powerless. Diabetes is generally divided into several types such as type I diabetes, type II diabetes and gestational diabetes. Diabetes can not only cause microvascular complications, such as peripheral neuropathy, eye disease, diabetic nephropathy, etc., but also serious macrovascular complications, such as myocardial infarction, stroke, and vascular obstructive l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P3/10A61K135/00
Inventor 朱元元朱晓丹白钢聂万达刘新元周德英
Owner NANKAI UNIV
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