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Corydalis saxicola bunting alkaloid extract and preparation method thereof, as well as extraction method of dehydrocavidine

A Coptis Rhizoma alkaloid and extract technology, applied in the biological field, can solve the problems of high labor intensity, environmental pollution, low yield, etc., and achieve the effect of increasing the content and improving the dissolution rate

Inactive Publication Date: 2012-07-25
NINGBO DELVERPHARM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This process has disadvantages such as low yield, high labor intensity, and easy to cause environmental pollution. Moreover, chloroform is used in the process, which seriously endangers the health of production personnel.

Method used

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  • Corydalis saxicola bunting alkaloid extract and preparation method thereof, as well as extraction method of dehydrocavidine
  • Corydalis saxicola bunting alkaloid extract and preparation method thereof, as well as extraction method of dehydrocavidine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of the crude total alkaloids of embodiment 1 Litectin

[0026] After the clean and dry rhizome is preliminarily crushed with a pulverizer, put it into the extraction tank, add 20 times the weight of sodium phosphate buffer solution (pH4.0, 0.2mol / L), raise the temperature to 45°C, add the compound enzyme (fiber Vegetase: pectinase=1:1), consumption is 2%, stir slowly, after keeping at temperature 45 ℃ for 8 hours, stop reaction, adjust pH value to 2.0 with dilute hydrochloric acid, keep warm for 8 hours, centrifuge at 4000r / min , to collect the extract. The extract is passed through an AB-8 macroporous adsorption resin column to absorb the total alkaloids, and the desorption eluent is 55% ethanol aqueous solution. The desorbed solution is recovered from ethanol and dried to obtain crude total alkaloids. The total alkaloid content in this crude total alkaloid is 65.8%. Wherein, the content of total alkaloids=total alkaloids / the crude total alkaloids ma...

Embodiment 2

[0028] The preparation of the crude total alkaloids of embodiment 2 Litectin

[0029] Put the clean and dry rock yellow with a pulverizer into the extraction tank, add 25 times the weight of sodium acetate-acetic acid buffer solution (pH4.5, 0.2mol / L), raise the temperature to 45°C, and add the compound enzyme (cellulase: pectinase = 1: 1), the dosage is 3%, stir slowly, keep at a temperature of 45°C for 8 hours, stop the reaction, adjust the pH value to 2.0 with dilute hydrochloric acid, keep warm for 8 hours, 4000r / min Centrifuge to collect the extract. The extract is passed through a DA-201 macroporous adsorption resin column to absorb the total alkaloids, and the desorption eluent is 50% ethanol aqueous solution. The desorbed solution is recovered from ethanol and dried to obtain crude total alkaloids. The total alkaloid content in this crude total alkaloid is 66.2%.

Embodiment 3

[0030] Example 3 is refined to obtain total alkaloids

[0031] The thick total alkaloid that embodiment 1 or embodiment 2 obtains is dissolved (pH3.0, 0.1mol / L) with the glycine-hydrochloric acid buffer solution (pH3.0, 0.1mol / L) of 10 times of amount, refines on the Sephadex LH-20 separation column, in detector The elution peaks of the alkaloids are collected under monitoring, and the refined and purified alkaloids are obtained after desalting and drying, with a content of 96.8%.

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PUM

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Abstract

The invention discloses a corydalis saxicola bunting alkaloid extract and a preparation method thereof, as well as an extraction method of dehydrocavidine on the basis the alkaloid extract. According to the method, in the raw material pretreatment, pectinase and cellulose perform enzyme hydrolysis to the medicament, so that the cellulose component and pectin component in plant tissues are destroyed, the solubility of effective components is improved, and the content of total alkaloids in the extract is greatly increased. The content of the total alkaloids extracted by using a twice chromatography method is over 95 percent, and only ethanol is used as a solvent; and the dehydrocavidine monomer is extracted from the total alkaloids by adopting a high-speed counter-current chromatography technology, and the purity is over 99 percent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, to an extract of alkaloids from Litectinus chinensis and a preparation method thereof, and a method for extracting dehydrocarvitin. Background technique [0002] Rock coptis (Corydalis saxicola Bunting), a plant of the genus Corydalis in the family Papaveraceae, is a perennial herb. It has significant analgesic, stable, antibacterial, anti-inflammatory, anti-tumor, anti-viral, choleretic, and immunomodulatory effects, and can be used clinically for acute jaundice hepatitis, viral hepatitis, chronic hepatitis, hyperbilirubinemia, liver Treatment of diseases such as cirrhosis and liver cancer. Its active ingredient is a variety of alkaloids mainly dehydrocavidine (dehydrocavidine), and the content of alkaloids directly affects the quality and curative effect of medicinal materials. [0003] The plant distribution of Yanhuanglian is limited to limestone mountainous areas, and it is an e...

Claims

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Application Information

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IPC IPC(8): A61K36/66C07D455/03A61P1/16A61P31/20A61P35/00
Inventor 胡玮
Owner NINGBO DELVERPHARM TECH
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