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Method for degrading vacuum gas oil by applying combination of phanerochete chrysosporium and bacillus

A technology of vacuum distillate oil and Phaneroderma chrysosporium, which is applied in the field of petrochemical industry, can solve the problem that the quantity of domestic crude oil cannot be satisfied, and achieve the effects of increased output rate, good application prospects and economic benefits

Active Publication Date: 2013-04-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the domestic crude oil is heavy, and most of the crude oil contains 40% to 50% of the vacuum residue > 500 ℃, and the quantity of domestic crude oil is increasingly unable to meet the needs of the rapid development of the national economy

Method used

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  • Method for degrading vacuum gas oil by applying combination of phanerochete chrysosporium and bacillus
  • Method for degrading vacuum gas oil by applying combination of phanerochete chrysosporium and bacillus
  • Method for degrading vacuum gas oil by applying combination of phanerochete chrysosporium and bacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Cultivation of vacuum distillate oil degrading bacteria

[0041] Bacterial strain A is inoculated in enriched PDA (recipe is: peeled potato 200g; Glucose 20g; KH 2 PO 4 3g, MgSO 4 ·7H 2 (1.5 g of water; 1000 mL of water; 18 g of agar; natural pH) plate, culture at 35° C. for 48 hours, after the plate is completely covered with white spores, inoculate 3 rings of spores into 50 mL of enriched PD liquid medium (formula: : Peeled potato 200g; glucose 20g; KH 2 PO 4 3g, MgSO 4 ·7H 2 O 1.5g; water 1000mL; pH natural) amplification culture, the culture condition is shaker culture, 35 ℃, 180rpm, 96 hours, when bacterial strain A grows into the bacterium ball that diameter is about 5mm, the bacterium solution is packed in 40mL In the centrifuge tube, centrifuge at 10,000 rpm, remove the supernatant, and keep the cells for later use.

[0042] Strain B was streak-inoculated on beef extract peptone solid medium (its formula (L -1 ): beef extract 5g; peptone 10g; NaCl 5g...

Embodiment 2

[0051] 1. Cultivation of vacuum distillate oil degrading bacteria

[0052] Same as embodiment one.

[0053] 2. Degradation of vacuum distillate oil

[0054] The vacuum fraction oil that adopts is vacuum fraction three, and others are with embodiment one.

[0055] 3. Gas chromatography analysis

[0056] Others are the same as embodiment one. Gas chromatographic analysis after degradation see image 3 and Figure 4 .

[0057] The analysis found that C33-C35 in the vacuum distillate oil had obvious degradation in different degrees, and other hydrocarbons also had different degrees of degradation, and the amount of short-chain hydrocarbons did not decrease. However, new hydrocarbon components are added between C24 and C26.

Embodiment 3

[0059] 1. Cultivation of vacuum distillate oil degrading bacteria

[0060] Same as embodiment one.

[0061] 2. Degradation of vacuum distillate oil

[0062] The vacuum distillate oil that adopts is vacuum distillate oil four, and others are with embodiment one.

[0063] 3. Gas chromatography analysis

[0064] Others are the same as embodiment one. Gas chromatographic analysis after degradation see Figure 5 and Figure 6 .

[0065] The analysis found that the content of linear alkanes below C29 in vacuum distillate oil IV increased overall, while the types of components between C31 and C36 increased, while the content of linear alkanes C36 decreased the most, and the content of C31 increased the most. It shows that after the vacuum distillate oil IV undergoes microbial degradation, the content of light oil increases.

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Abstract

The invention relates to a method for degrading vacuum gas oil by applying the combination of phanerochete chrysosporium and bacillus. The invention specifically separates bacillus pumilus SWS-0501 with the collection number of CGMCC (China General Microbiological Culture Collection Center) No. 3608. The invention further relates to a method for degrading the vacuum gas oil, which is characterized by combining the phanerochete chrysosporium and the bacillus pumilus to be used for degrading the vacuum gas oil.

Description

technical field [0001] The invention belongs to the field of petrochemical industry. More specifically, the present invention relates to the biodegradation of vacuum distillates, ie, to the degradation of vacuum distillates into lighter components by microorganisms. Background technique [0002] Petroleum is a very complex mixture of hydrocarbons and non-hydrocarbon compounds. To study or process crude oil, it is necessary to fractionate crude oil to obtain distillate oil. Distillates of crude oil mainly include gasoline, kerosene, aviation kerosene, diesel oil, lubricating oil, asphalt, etc. [0003] Looking at the history of the development of refining technology, the most basic driving force to promote the development of refining technology is how to produce petroleum products that can meet the constantly evolving quality and quantity requirements from crude oil with certain properties and compositions. In recent years, an important trend in refining technology is the t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C10G32/00C12R1/07
Inventor 杨树林艾凤祥赵健烽徐骏杨苏平
Owner CHINA PETROLEUM & CHEM CORP