Pyrethroid pesticide residue degradation bacteria as well as bacteria agent and application

A technology for the degradation of pyrethroids and pesticide residues. It is applied in the directions of bacteria, biochemical equipment and methods, and microorganisms. It can solve the problems of no degrading bacteria biological agents, difficult to degrade, and complex mechanisms, so as to promote the coordination and unification of diseases and insect pests. Prevention and treatment of drugs and food safety, low production cost, good degradation effect

Active Publication Date: 2013-05-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pyrethroid pesticides after the second generation are stable to light and heat, have a long half-life in the environment, and are difficult to degrade quickly under natural conditions. In addition, they are frequently used for a long time, and the problems and hazards related to their residues are becoming more and more serious.
[0005] However, the mechanism of using microorganisms to degrade pesticides is very complicated and highly targeted
Pyrethroid pesticides such as deltamethrin have been widely used in recent years, and no degrading bacteria and related biological agents specifically targeting such pesticides have been found

Method used

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  • Pyrethroid pesticide residue degradation bacteria as well as bacteria agent and application
  • Pyrethroid pesticide residue degradation bacteria as well as bacteria agent and application
  • Pyrethroid pesticide residue degradation bacteria as well as bacteria agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The acquisition of embodiment 1 degrading bacteria DG-02

[0028] In June 2010, the inventor isolated a degrading bacteria strain DG-02 from the soil of Dongguan City, Guangdong Province, China through a conventional enrichment culture method, and it was preserved in the China Type Culture Collection Center on November 8, 2011 ( The address is Luojia Mountain, Wuchang, Wuhan City, Hubei Province), the strain preservation number is CCTCC M 2011382, and it has been identified as Bacillus ( Bacillus sp.), the cells are rod-shaped, arranged in a chain, with spores; the colonies are white, opaque, with rough surfaces, large colonies, and irregular edges; contact enzyme test, methyl red test, acetylmethylmethanol test, nitrate Reduction test, starch hydrolysis test, gelatin liquefaction test, hydrogen sulfide test, casein hydrolysis test were positive; oxidase test, phenylalanine deaminase test, tyrosine hydrolysis test, indole test, glucose gas production test were negative;...

Embodiment 2

[0029] Embodiment 2 Preparation of degrading bacterial agent

[0030] The strain of pyrethroid pesticide residue degrading bacteria DG-02 of the present invention is activated on a solid plate, and its degradation performance is measured, and it is inoculated on the inclined surface of a test tube for later use. Inoculate the test tube seed of the pyrethroid pesticide residue-degrading bacteria DG-02 in a 1000 mL shake flask containing 250 mL of LB medium, shake at a constant temperature to the logarithmic phase, and prepare to inoculate the seed tank. The mass percentage of the LB medium consists of For: yeast extract 0.5%, peptone 1%, NaCl 1%; seed tank 100 L, feeding volume 80 L, medium formula: yeast extract 0.5%, peptone 1%, NaCl 1%, pH value 7.2-7.5. At 1.1 Kg / cm2 after feeding 3 Under the pressure of 121 ℃, high-pressure damp heat sterilization, after cooling to 30 ℃, inoculate the above cultured shake flask strains into 100 L seed tanks according to the inoculum amoun...

Embodiment 3

[0032] Example 3 Degradation experiment on pyrethroid pesticide residues

[0033] In basal salt medium (components: NH 4 NO 3 1.0 g, NaCl 0.5 g, K 2 HPO 4 1.5 g, KH2 PO 4 0.5 g, MgSO 4 . 7H 2 O 0.5 g, water 1000 mL, pH value 7.5) were added deltamethrin, fenpropathrin, lambda-cyhalothrin, lambda-cypermethrin, lambda-cyfluthrin and m-phenoxybenzoic acid to make the final The concentration was 50 mg / L; the concentration of the inoculated degrading bacteria DG-02 was 1.0×10 8 CFU / mL bacterial liquid, and the culture medium without inoculation was used as the control, cultured on a shaking table for 0-72 hours, samples were taken regularly, and the residual amount of pesticides was detected by high performance liquid chromatography (HPLC). The results are shown in Table 1. The degradation curve is attached figure 1 shown.

[0034]

[0035] Table 1 Degradation effect of degrading bacteria DG-02 on pyrethroid pesticides and m-phenoxybenzoic acid

[0036]

[0...

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Abstract

The invention discloses pyrethroid pesticide residue degradation bacteria as well as a bacteria agent and an application. A strain is Bacillussp. DG-02 with the strain preservation number of CCTCCM2011382. The degradation bacterial agent prepared by applying the Bacillussp. DG-02 is low in production cost, convenient to use and good in degradation effect, can be directly used to reduce the residual amount of deltamethrin, fenpropathrin, lambda-clyhalothrin, beta-cypermethrin, beta-cyfluthrin and 3-phenoxybenzoic acid in soil and crops by more than 75%, the problem of overproof pesticide residue in agricultural production is solved, and the degradation bacterial agent is suitable for being used in a large scale in fruit and vegetable production export bases across the country or places with green food brand marks, and has great significance in promoting the production of nuisance-free agricultural products and green food.

Description

technical field [0001] The invention belongs to the field of biological high technology, and relates to a technique for degrading chemical pesticide residues by means of microorganisms, in particular to a pyrethroid pesticide residue degrading bacteria and bacteria agent, and the production of green and pollution-free agricultural products in the field of modern agricultural production and processing applications. Background technique [0002] Chemical pesticides have made great contributions to the prevention and control of agricultural and forestry diseases, pests, weeds and rats. They are indispensable production substances in modern agriculture and forestry. However, pesticides are a double-edged sword. After they enter the natural environment, they will also bring great harm to the entire ecological environment. come to serious harm. Extensive use of pesticides has resulted in severe pesticide residues in some agricultural products and the environment, and the residues...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/07A62D101/04A62D101/28
Inventor 胡美英陈少华钟国华罗建军
Owner SOUTH CHINA AGRI UNIV
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