A kind of preparation method of daphnia steroidal saponin and flavonoid

A technology of steroidal saponins and galaxies, applied in the field of preparation of steroidal saponins and flavonoids of galaxies, can solve problems such as difficulty in separating alkaloids, and achieve the effects of controllable quality, increasing the number of particle layers formed, and high content

Active Publication Date: 2018-08-28
匡海学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Steroidal saponins and flavonoids are the main active ingredients of Dajinhua in treating psoriasis, and they are difficult to separate from alkaloids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing daphnia steroidal saponins and flavonoids, the composition of which comprises: the method is divided into two steps, the first step is to dissolve the medicinal material extract with a small amount of solvent to make a solution, and the solution passes through macroporous weakly alkaline acrylic acid It is an anion exchange resin, the solvent is eluted, and the solvent is recovered to obtain daphnia steroidal saponins.

[0025] The second step is to wash the macroporous weakly basic acrylic anion exchange resin with an alkaline solution, adjust the pH value of the eluent to neutral, and if the neutral eluent passes through the macroporous resin, rinse with water first, and discard the water eluent; Then elute with an ethanol solution, recover the ethanol eluent, and then obtain the flavonoids of datura flavonoids.

[0026] In the preparation method of the described datura steroidal saponins and flavonoids, the medicinal material extract in the fir...

Embodiment 2

[0033] The preparation method of dagenid saponin and flavone described in embodiment 1, above-mentioned order comprises the following steps:

[0034] (1) Dissolve the medicinal material extract in a small amount of water, pass through D941 macroporous weakly basic acrylic anion exchange resin or D900 macroporous weakly basic acrylic anion exchange resin or D318 macroporous weakly basic acrylic anion exchange resin or WDA918 macroporous weakly basic acrylic anion exchange resin, eluted with eluent 3 times to 20 times the volume of the resin bed, combined eluent, concentrated under reduced pressure, dried to obtain daphnia steroidal saponins;

[0035] (2) Use 3 times to 20 times of the volume of the resin bed to use the alkaline solution pH value of 8 to 14 ammonia or the sodium hydroxide of 0.1% to 3% to wash the macroporous weakly basic acrylic anion exchange resin, wash Use hydrochloric acid to adjust the pH value to 6.8-7.0 for dehydration, pass the eluate after adjusting th...

Embodiment 3

[0037] The preparation method of dagenid steroid saponin and flavonoid described in embodiment 1 or 2, its composition comprises:, this method is divided into two steps, and the first step is to dissolve the medicinal material extract with a small amount of solvent to make a solution, and the solution is passed through The macroporous weakly basic acrylic anion exchange resin is used to elute the solvent and recover the solvent to obtain daphnia steroidal saponins.

[0038] The second step is to wash the macroporous weakly basic acrylic anion exchange resin with an alkaline solution, adjust the pH value of the eluent to neutral, and if the neutral eluent passes through the macroporous resin, rinse with water first, and discard the water eluent; Then elute with an ethanol solution, recover the ethanol eluent, and then obtain the flavonoids of datura flavonoids.

[0039] In the preparation method of the described datura steroidal saponins and flavonoids, the medicinal material e...

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PUM

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Abstract

The invention relates to a preparation method of steroid in a traditional Chinese medicine: flos daturae, which can treat psoriasis. The flos daturae is the dry flower of datura metel L. which belongs to the family of solananeae and also named as datura stramonium flower, mandrake flower, brachybotrys paridiformis, solanumcoagulans forsk, white datura or the like. The preparation method comprises the following steps: 1, dissolving an flos daturae extract in a small amount of solvent, loading the flos daturae solution on a macroporous weak-basic acrylic acid type anion exchange resin, eluting by a solvent, and recovering the solvent to obtain the steroid saponin type component in the flos daturae; and 2, eluting the macroporous weak-basic acrylic acid type anion exchange resin by an alkaline solution, adjusting the pH value of the eluate to be neutral, loading to macroporous resin, firstly eluting by water, discarding the water eluate, eluting by a solution containing alcohol, and recovering the alcohol eluate to obtain the flavone type component in the flos daturae. The preparation method is used for preparing the steroid in the flos daturae.

Description

Technical field: [0001] The invention relates to a preparation method of daphronoid steroid saponins and flavonoids. Background technique: [0002] Datura datura is the dried flower of Datura metel L., a plant of the genus Solanaceae Datura. Steroidal saponins and flavonoids are the main active ingredients of Dajinhua for treating psoriasis, and they are difficult to separate from alkaloids. Therefore, it is of great significance to study more effective and stable extraction and separation methods of daphnia steroidal saponins and flavonoids. [0003] The steroidal components found in the flowers, leaves, stems, roots, and whole plants of Datura datura are: 5α, 27-dihydroxy-6α, 7α-epoxy-1-one-withandia- 2,24-dienolactone; 5α,12β,27-trihydroxy-6α,7α-epoxy-1-keto-withan-2,24-dienolactone; 5α,12α,27-trihydroxy- 6α, 7α-epoxy-(20R,22R)-1-keto-withan-2,24-dienolactone-27-O-β-D-glucopyranoside; 5α,6β,21,27- Tetrahydroxy-1-keto-withan-2,24-dienolactone; 5α, 6β, 21-trihydroxy-1-k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/81A61P17/06A61P29/00A61P37/08A61P17/04A61P11/06
Inventor 匡海学杨炳友王秋红王知斌王雪晶
Owner 匡海学
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