Compound magnetic biological adsorbent and preparation method for same

A biosorbent and composite technology, which is applied in the field of composite biosorbent and its preparation, can solve the problems of difficult recycling and repeated use, decreased processing efficiency, and difficulty in maintaining activity, so as to enhance the internal transmission and adsorption mechanism, reduce the Toxic, easy to make effects

Inactive Publication Date: 2012-08-01
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the traditional biosorption process, biological cells are generally in a free state, and are easily lost from the reactor and discharged with the liquid to be treated. It is difficult to recover and reuse by solid-liquid separation
In addition, the wastewater to be treated is often highly toxic to cells, and the activity of biological cells in a free state is difficult to maintain, resulting in a gradual decline in treatment efficiency.

Method used

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  • Compound magnetic biological adsorbent and preparation method for same
  • Compound magnetic biological adsorbent and preparation method for same
  • Compound magnetic biological adsorbent and preparation method for same

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A composite magnetic biosorbent of the present invention, the composite magnetic biosorbent is based on the mycelium of white rot fungus live fungus as a carrier, the mycelium is wound into a bacterium ball, and the inside of the bacterium ball is embedded with Fe 3 o 4 Nanoparticles and calcium alginate, calcium alginate as the immobilization medium will mycelium and Fe 3 o 4 Nanoparticles are tightly linked together. Fe embedded in the spheroid 3 o 4 The mass ratio of nanoparticles and calcium alginate is 1:1.2.

[0029] The preparation method of the composite magnetic biosorbent of the present embodiment comprises the following steps:

[0030] (1) Sterile Fe 3 o 4 Preparation of particles and sodium alginate mixture: 0.10g Fe 3 o 4 Nanoparticles were added to 2ml of sodium alginate solution with a concentration of 60 g / L, stirred well, then sterilized at 115°C for 30 minutes, and finally cooled under sterile conditions for 60 minutes to prepare sterile Fe 3...

Embodiment 2

[0036] A composite magnetic biosorbent of the present invention, the composite magnetic biosorbent is based on the mycelium of white rot fungus live fungus as a carrier, the mycelium is wound into a bacterium ball, and the inside of the bacterium ball is embedded with Fe 3 o 4 Nanoparticles and calcium alginate, calcium alginate as the immobilization medium will mycelium and Fe 3 o 4 Nanoparticles are tightly linked together. Fe embedded in the spheroid 3 o 4 The mass ratio of nanoparticles and calcium alginate is 1:0.4.

[0037] The preparation method of the composite magnetic biosorbent of the present embodiment comprises the following steps:

[0038] (1) Sterile Fe 3 o 4 Preparation of particles and sodium alginate mixture: 0.20g Fe 3 o 4 Nanoparticles were added to 2ml of sodium alginate solution with a concentration of 40g / L, stirred well, then sterilized at 105°C for 30min, and finally cooled for 80min under sterile conditions to obtain sterile Fe 3 o 4 Partic...

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Abstract

The invention discloses a compound magnetic biological adsorbent, which is characterized in that dry mycelia of white rot fungi are used as a carrier, the mycelia are wound to a ball, Fe3O4 nano particles and calcium alginate are embedded into the ball, and the calcium alginate serving as immobilized medium is tightly connected together with the Fe3O4 nano particles. The preparation method for the adsorbent includes the steps: inoculating spore suspension of the white rot fungi into aseptic mixed liquid of the Fe3O4 particles and the calcium alginate under the aseptic condition, mixing uniformly, dropwise adding the mixed liquid of one volume into aseptic CaC12 solution of 4-10 volumes, standing at the room temperature to obtain reaction liquid including Fe3O4-calcium alginate-white-rot fungus microspheres, and performing immobilized culture so as to obtain the compound magnetic biological adsorbent after culture is completed. The compound magnetic biological adsorbent has the advantages of high adsorption capacity, high adsorption speed, high reuse rate, cleanliness, no pollution and the like.

Description

technical field [0001] The invention belongs to the field of biological adsorption treatment of waste water, in particular to a composite biological adsorbent and a preparation method thereof. Background technique [0002] With the acceleration of urbanization and the rapid development of industry and agriculture, most cities in my country have serious water pollution problems. A large amount of untreated urban garbage, industrial waste water, domestic sewage and atmospheric sediments are continuously discharged into the water body, causing the heavy metal content in the suspended matter and sediment of the water body to rise sharply. Water heavy metal pollution has become a global environmental pollution problem. The treatment of heavy metal polluted water bodies is imminent. [0003] Lead-containing wastewater comes from various battery workshops, mineral processing plants, petrochemical plants, etc. During the production and operation of these industrial and mining enter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30C02F3/34C12N11/14C12N11/10C12R1/645
Inventor 许飘曾茁桐曾光明黄丹莲赵美花赖萃危臻李宁杰黄超张辰李芳玲
Owner HUNAN UNIV
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