Mycobacterium tuberculosis rifampin-resistance mutation visual detection probes and application thereof

A technology for Mycobacterium tuberculosis and drug-resistant mutations, applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc., can solve problems such as complex operations

Inactive Publication Date: 2012-08-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods currently used by researchers to detect drug-resistant mutations in Mycobacterium tuberculosis include molecular beacons and reverse probe hybridization, but these two methods are more complicated to operate and require specialized instruments

Method used

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  • Mycobacterium tuberculosis rifampin-resistance mutation visual detection probes and application thereof
  • Mycobacterium tuberculosis rifampin-resistance mutation visual detection probes and application thereof
  • Mycobacterium tuberculosis rifampin-resistance mutation visual detection probes and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Design of DNA peroxidase mutation detection probe system

[0063] (1) The sequence of the core region of the rpoB gene mutation is as follows: figure 1 shown

[0064] The present invention comprises 22 mutation types of 510 to 524 amino acids ( figure 1 underlined), the specific mutations and corresponding detection probes are as follows:

[0065] probe cluster

mutation site

mutation type

Corresponding detection probe

1

510

G→C

B510-3G

1

511

C→T

B511-1A

1

511

T→C

B511-2G

1

511

T→G

B511-2C

1

513

C→A

B513-1T

1

513

A→C

B513-2G

1

513

A→G

B513-2C

2

515

A→G

B515-1C

2

515

G→T

B515-3A

2

516

G→A

B516-1T

2

516

G→T

B516-1A

2

516

A→C

B516-2G

2

516

A→G

B516-2C

...

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Abstract

The invention discloses mycobacterium tuberculosis rifampin-resistance mutation visual detection probes and an application thereof. For rifampin-resistant mutation, mycobacterium tuberculosis rifampin-resistant rpoB gene mutation core region 510-524 amino acids are divided into three regions; three detection probe clusters are designed and synthesized based on all mutation sites included in every region; each probe cluster includes several probe sets, each probe set comprises specific mutation detection B probes. Various probe sets and probe clusters designed in the invention have great discrimination performance for synthesized wild-type and mutant-type detection fragments, can accurately detect the specific mutation sites of the rifampin-resistant mycobacterium tuberculosis rpoB gene mutation core region 510-524 amino acids and have a good application prospect.

Description

technical field [0001] The invention belongs to the fields of pathogenic biology, molecular microbiology and nucleic acid chemistry, and relates to a design method and sequence of a novel gene point mutation detection probe, and its application in the detection of rifampicin-resistant mutations of Mycobacterium tuberculosis. This method can intuitively, rapidly and visually detect rifampicin-resistant mutations in Mycobacterium tuberculosis. Background technique [0002] Tuberculosis (TB for short) caused by Mycobacterium tuberculosis is an ancient disease that has been widely spread in the world long ago. Since the 1950s, effective drugs such as isoniazid and rifampicin have been discovered for the treatment of tuberculosis. , greatly improved the cure rate of tuberculosis, and reduced the recurrence rate of tuberculosis, making tuberculosis a curable and controllable disease. In some developed countries, tuberculosis has been largely brought under control. However, in re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12N15/11
Inventor 章晓联周翔邓明刚冯烁罗凤玲孙小明
Owner WUHAN UNIV
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