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Medicine composition for reversing cancer cell tolerance and application of medicine composition

A technology for tumor cells and tumor drugs, which is applied in the field of pharmaceutical compositions for reversing drug resistance of tumor cells, and can solve problems such as drugs for reversing drug resistance of tumor cells

Active Publication Date: 2012-08-15
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, in clinical application, there is no drug that can effectively reverse the drug resistance of tumor cells

Method used

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  • Medicine composition for reversing cancer cell tolerance and application of medicine composition
  • Medicine composition for reversing cancer cell tolerance and application of medicine composition
  • Medicine composition for reversing cancer cell tolerance and application of medicine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0184] Example 1 Effect of Corydaline on the Ratio of Side Group Cells of Hepatocellular Carcinoma Cell Lines

[0185] 1.1 Detection of the effect of isocydine and doxorubicin on the proportion of side population cells in hepatocellular carcinoma cell lines

[0186] 1.1.1 Flow cytometry was used to detect the changes in the proportion of side population cells in the hepatocellular carcinoma cell lines of the isorydine treatment group and the control group.

[0187] Control group: MHCC-97L, MHCC-97H and MHCC-LM324h were treated with two different concentrations of isocydine (150 μg / ml, 250 μg / ml).

[0188] Blank control group: MHCC-97L, MHCC-97H and MHCC-LM324h were treated with PBS.

[0189] Negative control group: MHCC-97L, MHCC-97H and MHCC-LM324h were treated with FTC.

[0190] The results showed that: compared with the blank control group, the proportion of side population cells in the hepatocellular carcinoma cells in the treatment group decreased significantly.

[019...

Embodiment 2

[0212] Example 2 Isocydine has a stronger growth inhibitory effect on side population cells

[0213] Sorting of side population cells and non-side population cells

[0214] The side population cells and non-side population cells were sorted from MHCC-97L, see method 2.2 for the sorting method, and the purity of the sorting was tested. The purity of the sorted side population cells and non-side population cells all reached more than 99% (such as Figure 3A shown), the total protein of the sorted cells was extracted, and the expression level of ABCG2 was detected by Western blot, and ABCG2 was relatively highly expressed in the side population cells (such as Figure 3B shown), further proving that the sorting was successful.

[0215] 2.1 BrdU cell proliferation assay

[0216] The sorted side population cells and non-side population cells were inoculated into 96-well plates, and the above two kinds of cells were treated with 50 μg / ml, 100 μg / ml, 150 μg / ml and 200 μg / ml of isoc...

Embodiment 3

[0245] Example 3 Effect of Corydaline on MHCC-97L Side Population Cell Subcutaneous Tumor Formation in Nude Mice

[0246] Side group cells and non-side group cells were sorted from MHCC-97L cells, and inoculated subcutaneously in nude mice respectively. After tumor formation, they were divided into two groups:

[0247] Treatment group: including side population cell subcutaneous tumor formation treatment group and non-side population cell subcutaneous tumor formation treatment group;

[0248] Each mouse in the treatment group was given isocydine solution (0.4 mg / 200 μl PBS) by tail vein injection respectively; 5 times a week, after 4 weeks of administration, the two groups of nude mice were sacrificed without pain. Tumor tissue was removed.

[0249] Control group: including a side group cell subcutaneous tumor formation control group and a non-side group cell subcutaneous tumor formation control group;

[0250] PBS was used instead of cordydine as a control, and after 4 week...

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Abstract

The invention relates to a medicine composition for reversing cancel cell tolerance and application of the medicine composition, and particularly discloses an isocorydin (ICD) and a pharmaceutically acceptable novel use of salt of the isocorydin (ICD), which are used for preparing the medicine composition for treating cancer. The medicine composition can increase sensitivity of cancer cells to anti-cancer medicines, or reduce cancel cell tolerance to the anti-cancer medicines.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a pharmaceutical composition for reversing drug resistance of tumor cells and its application. Background technique [0002] Hepatocellular carcinoma (HCC) is the fifth most common malignant tumor and the third in the number of deaths caused by tumors. About 600,000 people die of HCC every year, which seriously threatens human health. Resistance to traditional chemotherapeutic drugs is an important factor in the unsuccessful treatment of hepatocellular carcinoma. The generation of tumor cell drug resistance is a complex process involving many factors, one of which is the high expression of ATP-dependent transporter family proteins in tumor cells. [0003] ABCG2 is an important member of this family and is closely related to the diverse drug resistance of tumors. As a transporter, it can efflux many commonly used chemotherapeutic drugs, such as doxorubicin, daunorubicin, mi...

Claims

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Application Information

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IPC IPC(8): A61K31/473A61P35/00
Inventor 李锦军路平
Owner SHANGHAI INST OF ONCOLOGY
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