Microbe strain and application thereof

A technology of microorganisms and strains, applied in the directions of microorganisms, microorganism-based methods, applications, etc., can solve problems such as affecting the burning speed and completeness of combustion, affecting the aroma quality and safety of tobacco leaves, and reducing the flammability of tobacco leaves, so as to improve the availability of tobacco leaves. , the effect of reducing irritation, protein and starch content

Active Publication Date: 2014-03-12
CHINA TOBACCO GUANGXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sugars in the form of starch will affect the burning speed and completeness of burning when cigarettes are smoked. When burning, they will produce burnt smell, which will make the flavor of tobacco worse.
Protein is the basic material of cells. Its hydrolyzed products and further transformed products are the original substances of many tobacco aroma substances, but when the protein content is high, it will produce a protein odor like burning feathers, which reduces the burning of tobacco leaves. It is a precursor of harmful substances in smoke, including quinine, HCN, and other nitrogen-containing compounds, which seriously affect the flavor quality and safety of tobacco leaves

Method used

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  • Microbe strain and application thereof
  • Microbe strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Slant culture: 10g peptone, 5g yeast extract, 10g sodium chloride, 15g agar, 1000ml distilled water, pH7.0, inoculate the cryopreserved glycerol suspension on the slant medium, and incubate at 30°C for 2 days.

[0031]Shake flask culture: nicotine 1g, peptone 5g, yeast extract 5g, sodium chloride 5g, K 2 HPO 4 ·3H 2 O13.3g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O0.2g, 0.5ml of trace elements, add 1000ml of distilled water, adjust the pH value to 7.0, and sterilize by autoclaving. The volume of liquid in a 250ml shaker flask is 30ml, the inoculum size is 3v / v%, the culture temperature is 30°C, the shaker speed is 200rpm, and the shaker flask is cultivated for 36 hours, the nicotine degradation rate reaches 100%, the protease activity reaches 28U / ml, and the starch The enzyme activity reaches 36U / ml. Trace elements: CaCl 2 0.04g, CuSO 4 0.07g, MnSO 4 ·H 2 O0.008g, FeSO 4 ·7H 2 O0.004g, Na 2 MoO 4 2H 2 O0.1g, add 0.1mol L -1 Dissolve in HCl to a volume of 100ml.

[...

Embodiment 2

[0035] The slant culture was the same as in Example 1. Fill a 250ml shaker flask with 30ml medium, the composition of which is as follows: nicotine 2g, corn steep liquor 15g, yeast extract 5g, sodium chloride 5g, K 2 HPO 4 ·3H 2 O13.3g, KH 2 PO 4 4g, MgSO 4 ·7H 2 O0.2g, 0.5ml of trace elements, add 1000ml of distilled water, adjust the pH value to 7.0, and sterilize by autoclaving. The volume of liquid in a 250ml shaker flask is 30ml, the inoculum size is 2v / v%, the culture temperature is 30°C, the shaker speed is 200rpm, and the shaker flask is cultivated for 48 hours. The nicotine degradation rate reaches 100%, the protease activity reaches 54U / ml, and the starch The enzyme activity reaches 28U / ml. Trace element is identical with embodiment 1. The definition of amylase activity and protease activity is the same as in Example 1.

Embodiment 3

[0037] Get the fermented liquid 10ml among the embodiment 1, dilute to 10ml with sterile distilled water 9 Bacteria / ml, the bacterial solution is sprayed to 2008 Shaoyang upper tobacco leaves B2F with a water content of 18% according to 1% of the weight of the tobacco leaves, and fermented for 10 hours at 35°C and a relative humidity of 75%. The content of nicotine decreased by 2.9%, the content of protein decreased by 5.6%, and the content of starch decreased by 10.8%.

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Abstract

The invention discloses a microbe strain and an application thereof and particuarly relates to a tobacco smoke alkaloid degradation bacterium (Arthrobacter nicotianae GYC103 with the collection number of CCTCC NO:M2010312) capable of simultaneously generating amylolytic enzyme and proteolytic enzyme and an application of the tobacco smoke alkaloid degradation bacterium to tobacco. The strain disclosed by the invention can decompose and utilize nicotine and generate amylolytic enzyme and proteolytic enzyme in a growth process. A strain fermentation liquid or thallus accounting for 1-5wt% of tobacco leaves is added into the tobacco leaves with the water content of 10-50%, and fermentation is performed for 6-72 hours, so that the nicotine content of the tobacco leaves is reduced by 2-20%, the starch content of the tobacco leaves is reduced by 10-35%, the protein content of the tobacco leaves is reduced by 5-25%, the pungency of the tobacco leaves is obviously reduced, the miscellaneous flavor is reduced, the smoke is soft, the tobacco fragrance is enhanced, and the esthetic quality is obviously improved. The microbe strain realizes the purpose of degrading tobacco smoke alkaloid, starch and protein by utilizing microbes, and can be used for properly regulating the contents of nicotine, starch and protein in tobacco leaf raw materials and improving the usability of the tobacco leaves.

Description

technical field [0001] The present invention relates to a microbial strain and its application, in particular to a tobacco nicotine-degrading bacterium capable of producing both amylase and protease - Arthrobacter nicotianae GYC103 CCTCC NO: M2010312 and its application in tobacco. The invention belongs to the technical field of microbe application. Background technique [0002] Nicotine, also known as nicotine, is the main component of tobacco alkaloids. The content of nicotine in flue-cured tobacco is generally 1.5% to 3.5%, preferably about 2.5%. If the nicotine content is too low, the energy will be too small; if the nicotine content is too high, the energy will be too large, which will cause a tingling and spicy feeling. In recent years, the appearance quality of tobacco leaves in some areas of my country is close to the international level, but the chemical composition of tobacco leaves is not harmonious, and the nicotine is high, especially in the upper tobacco leav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/06
Inventor 龙章德黄泰松韦建玉白森邹克兴胡亚杰蔡联合金亚波张纪利
Owner CHINA TOBACCO GUANGXI IND
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