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Testis live sperm collector

A technology of sperm collector and collector, applied in biochemical instruments, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc. Cross-contamination and other issues, to achieve the effect of easy mastering, short grinding time and low cost

Inactive Publication Date: 2012-09-12
陈子江 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the manual peeling and crushing steps, there are many deficiencies: 1. In the manual peeling and crushing process, the safety is poor, and the sample is easily knocked over
2. The in vitro operation time is too long and the temperature drops significantly, which affects the activity of testicular sperm in the sample
3. Samples are prone to cross-contamination
4. The operation steps and the pre- and post-cleaning are cumbersome

Method used

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  • Testis live sperm collector
  • Testis live sperm collector
  • Testis live sperm collector

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] The cylindrical edge 1 of the collector, the outer clamping feet 3, the inner clamping feet 4 and the grinding net 2 are integrally formed, and the material is polypropylene (PP) plastic. The outer clamping feet 3 are perpendicular to the plane of the cylindrical edge 1, and the inner clamping feet 4 are also perpendicular to the plane of the cylindrical edge 1. Between the outer clamping feet 3 and the inner clamping feet 4 A card slot 5 with a substantially rectangular cross section is formed. The width of the card slot 5 is 2mm. The lengths of the outer clamping feet 3 and the inner clamping feet 4 are respectively 5mm. The grinding net 2 is in a concave shape, and the mesh area on it is located at the center of the cylindrical edge 1, and the hole diameter of the mesh is 75 μm. Choose to use the carrying container 7 whose protruding edge (vertical) can be in close contact with the card slot 5 .

[0061] When in use, first add a small amount of reagents or other s...

Embodiment 2

[0063] The cylindrical edge 1, the outer clip 3, the inner clip 4 and the grinding net 2 of the collector are integrally formed, and the material is aluminum-magnesium alloy. The outer clip 3 is perpendicular to the plane of the cylindrical edge 1, the angle between the inner clip 4 and the plane of the cylindrical edge 1 is 45 degrees, and the outer clip 3 and the A card slot 5 with a substantially triangular cross-section is formed between the inner clip feet 4 . The lengths of the outer clamping feet 3 and the inner clamping feet 4 are respectively 5mm. The grinding net 2 is in the shape of a plane, and the mesh area on it is located at the center of the cylindrical edge 1, and the hole diameter of the mesh is 75 μm. Choose to use the carrying container 7 whose protruding edge (vertical) can be in close contact with the deepest part of the card slot 5 or choose to use the carrying container 7 whose protruding edge (vertical) can be in close contact with the inner clip 4 . ...

Embodiment 3

[0066] The cylindrical edge 1 of the collector, the outer clip 3 and the inner clip 4 are integrally formed, and the material is polyethylene (PE). The material of the grinding net 2 is magnesium aluminum alloy, and is connected with the cylindrical edge 1 Lap fastened. The angle between the outer clip 3 and the plane of the cylindrical edge 1 is 100 degrees (pointing to the outside of the cylindrical edge 1), the inner clip 4 and the plane of the cylindrical edge 1 The angle between them is 80 degrees (pointing to the inner side of the cylindrical edge 1 ), and a card slot 5 with a substantially triangular cross-section is formed between the outer clip 3 and the inner clip 4 . The lengths of the outer clamping feet 3 and the inner clamping feet 4 are respectively 5mm. The grinding net 2 is in a concave shape, and the mesh area on it is located at the center of the cylindrical edge 1, and the hole diameter of the mesh is 75 μm. Choose to use the carrying container 7 whose pr...

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Abstract

The invention relates to a testis live sperm collector comprising a tubular edge, outer clamping feet and inner clamping feet, wherein a grinding net is fixed in the tubular edge, the outer and inner clamping feet are arranged at the lower parts of the tubular edge, and a clamping groove is formed between each outer clamping foot and each inner clamping foot. The testis live sperm collector has the beneficial effects that as the design is rational, the collector can be matched with a majority of bearing utensils used in experiments and tests; as the operation is simple and easy to master, the time for the operation is reduced; as the grinding time is short, the influence of mechanical stress on samples is small; and as the cost is relatively low, the collector can be used for once and discarded after use, thereby avoiding cross infection of the samples.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a testicular tissue live sperm collector. Background technique [0002] At present, in teaching experiments and medical examinations, for the collection of microscopic testicular tissue living sperm, the collection method is not convenient to use grinding machinery for mechanical grinding due to the microscopic characteristics of the sample (for example, the general sampling volume is less than 0.5cm in length) . The current method is mainly to peel off and crush the seminiferous tubules of the testicular tissue by hand, and then collect them by centrifugation. In the manual peeling and crushing steps, there are many deficiencies: 1. In the manual peeling and crushing process, the safety is poor, and the samples are easily knocked over. 2. The in vitro operation time is too long and the temperature drops significantly, which affects the activity of testicular sperm in the sample. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/33
Inventor 陈子江李城吴克良马金龙
Owner 陈子江