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Mass spectrometry method based on single-pixel solid-phase extraction technology and application thereof

A mass spectrometry imaging, single-pixel technology, applied in the field of mass spectrometry, can solve the problem of low imaging sensitivity

Active Publication Date: 2017-02-22
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the deficiencies of the above-mentioned prior art, the present invention provides a mass spectrometry imaging method based on single-pixel solid-phase extraction technology, thereby effectively solving the problem of low imaging sensitivity of low-abundance components on the surface of complex samples

Method used

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  • Mass spectrometry method based on single-pixel solid-phase extraction technology and application thereof
  • Mass spectrometry method based on single-pixel solid-phase extraction technology and application thereof
  • Mass spectrometry method based on single-pixel solid-phase extraction technology and application thereof

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Embodiment 1

[0058] Such as figure 2 As shown, a single-pixel solid-phase extraction array plate used for sample pretreatment in mass spectrometry imaging, the single-pixel solid-phase extraction array plate includes a matrix membrane 3, and the material of the matrix membrane 3 is silicone gel-Teflon Dragon film, its thickness is 0.12mm.

[0059] The matrix membrane 3 is provided with several tapered through holes 1 arranged at a pitch of 100 μm, which means that the circle center of one of the tapered through holes and the circle center of the adjacent tapered through hole are 100 μm. Such as figure 1 Shown, the diameter (L 1 ) is 80μm, the diameter of the opening at the other end (L 2 ) is 30 μm.

[0060] Adsorption materials 2 that are adsorbed to target molecules to be mass spectrometered in plant tissues are loaded in the tapered through holes 1 . The selection of the adsorption material 2 can be determined according to the properties of the target molecules to be imaged in the...

Embodiment 2

[0065] A preprocessing method for mass spectrometry imaging based on single-pixel solid-phase extraction technology, comprising the following steps:

[0066] (1) Transfer and imprinting of chemical components in plant tissue slices: the fresh plant tissue slices are selected as 5mm in thickness, and after freezing, they are quickly combined with the surface of the single-pixel solid-phase extraction array plate in Example 1 (the tapered through hole is opened at one end) The larger side) is docked and pressed for about 5 seconds, and then the tissue slices are peeled off. At this time, the chemical components in the plant tissue slices are transferred to the tapered through-holes on the single-pixel solid-phase extraction array plate to realize the chemical components in the plant tissue slices. chemical component transfer and imprinting;

[0067] (2) Purification in the tapered through-holes: use an organic solvent (such as a mixed solution of methanol and water) to elute in ...

Embodiment 3

[0070] A mass spectrometry imaging system based on single-pixel solid-phase extraction technology, the device includes a nanoliter spray device, a slice imprinting device, the single-pixel solid-phase extraction array plate in embodiment 1, a single-pixel purification device, a camera and a mass spectrometer The slice imprinting device is used to realize the transfer and imprinting of chemical components of plant slices; the single-pixel solid-phase extraction array plate and single-pixel purification device are used for in-situ solid-phase extraction of plant tissue slice imprints, for Regulate the chemical composition in the single pixel; the spray device is used to obtain the target chemical composition of each pixel in the process of mass spectrometry image acquisition; the camera is placed on both sides of the solid phase extraction plate, and is used to record the single pixel extraction process and the mass spectrometry signal acquisition process; The mass spectrometer i...

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Abstract

The invention discloses a mass spectrometry method based on a single-pixel solid-phase extraction technology and application thereof. The mass spectrometry method comprises the following steps: (1) spraying a solvent for desorbing target molecules into a purified conical through hole in a spray form of a spray, enabling the spray to run through the conical through hole, desorbing the target molecules to obtain a secondary spray comprising the target molecules, enabling the secondary spray to enter a sample feeding port of a mass spectrometer so as to finish sample feeding of one conical through hole, and similarly finishing sample feeding of a next conical through hole so as to finish mass spectrometry on each conical through hole by the mass spectrometer; (2) performing sample collection and data analysis by the mass spectrometer so as to acquire a mass spectrum image of component ions. The mass spectrometry method adopts the single-pixel solid-phase extraction technology innovatively; through intra-pixel point pretreatment, an inhibiting phenomenon of a complex matrix on the ions of the target molecules is effectively avoided, so that influence of complex matrix interference on the accuracy of the mass spectrum image is practically avoided and the sensitivity of low-abundance component imaging is improved.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry, and in particular relates to a mass spectrometry imaging analysis method based on single-pixel solid-phase extraction technology and its application. Background technique [0002] Traditional molecular imaging techniques, such as spectroscopy using radioactive isotopes or fluorescent labels, are time-consuming and laborious, and the labels may dissociate during the imaging process, while "label-free" imaging spectroscopy methods have weak luminescent signals and low background Disadvantages such as strong interference. Mass spectrometry imaging (MSI) is a new type of imaging technology. Using this technology, the spatial distribution information of various proteins or small molecular metabolites can be obtained directly from the surface of biological tissue slices. The principle of this in situ analysis technique is to use laser or ion beam to ionize the molecules on the surface of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64B01D15/10
CPCB01D15/10G01N27/64
Inventor 陈相峰陈德华李婉
Owner SHANDONG ANALYSIS & TEST CENT
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