Mixed plant protein fish feed as well as preparation method and application thereof

A technology of mixing plants and fish feeds, applied in animal feeds, animal feeds, applications, etc., can solve the problems of single enzyme system, slow fermentation speed, amino acid imbalance between rapeseed meal and cotton meal, etc., and achieve strong anti-bacterial ability, High fermentation efficiency and high conversion effect

Inactive Publication Date: 2012-09-19
WUHAN RICH GREEN AGRI BIOLOGICAL ENG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The vegetable protein feed prepared by the existing miscellaneous meal fermentation technology mostly uses single bacteria or two strains of mixed fermentation to process the miscellaneous meal. The enzyme system is relatively simple,

Method used

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  • Mixed plant protein fish feed as well as preparation method and application thereof
  • Mixed plant protein fish feed as well as preparation method and application thereof
  • Mixed plant protein fish feed as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of Preliminary Example 1 Bacillus subtilis Fermented Liquid

[0033] 1. Seed cultivation

[0034] After activating the Bacillus subtilis strain, carry out shake flask seed culture;

[0035] The composition of shake flask seed culture medium (by mass percentage):

[0036] 20% potato + 2% sucrose PDA medium;

[0037] Culture conditions: 150r / min, 37°C shaker culture for 18 hours.

[0038] 2. Secondary liquid expansion culture

[0039] Liquid fermentation medium: 3% corn flour, 2% amino acid powder, 0.5% potassium dihydrogen phosphate, 1% calcium chloride, 1% sucrose; sterilize the above liquid medium fermentation tank in place;

[0040] Liquid expansion culture conditions: fermentation temperature is 37°C, the oxygen flow rate can be 100-300L / h, initial pH: 7.0-7.5; speed: 150-200r / min; tank pressure: 0.6-0.8Mpa, fermentation culture 24 Hours, the general effective number of viable bacteria up to 10 9 CFU / ML is enough.

[0041] 3. Liquid fermentation cu...

Embodiment 2

[0044] Preparation of Preparatory Example 2 Enterococcus faecalis Fermented Liquid

[0045] 1. Seed cultivation

[0046] After activating Enterococcus faecalis strains, carry out shake flask seed culture;

[0047] The composition of shake flask seed culture medium (by mass percentage):

[0048] Medium composition: glucose 2%, peptone 1%, yeast extract 0.5%;

[0049] Culture conditions: 150r / min, 32°C shaker culture for 18 hours.

[0050] 2. Secondary liquid expansion culture

[0051] Liquid fermentation medium: 3% soybean meal powder, 3% amino acid powder, 0.5% dipotassium hydrogen phosphate, 2% calcium oxide; sterilize the above liquid medium fermentation tank in place;

[0052] Liquid expansion culture conditions: fermentation temperature is 32°C, its oxygen flow can be 50-100L / h, initial pH: 7.5-8.0; speed: 150-200r / min; tank pressure: 0.1-0.3Mpa, fermentation culture 24 Hours, the general effective number of viable bacteria up to 10 9 CFU / ML is enough.

[0053] 3. L...

Embodiment 3

[0056] Preparation of Preliminary Example 3 Aspergillus Niger Fermented Liquid

[0057] 1. Seed cultivation

[0058] After activating the Aspergillus niger strain, carry out shake flask seed culture;

[0059] The composition of shake flask seed culture medium (by mass percentage):

[0060] 20% potato + 2% sucrose PDA medium;

[0061] Culture conditions: 150r / min, shaker at 28°C for 18 hours.

[0062] 2. Secondary liquid expansion culture

[0063] Liquid fermentation medium: 10% potato, 0.5% diammonium citrate, 0.2% magnesium chloride, 2% sucrose; in-situ sterilization in the above-mentioned liquid medium fermenter;

[0064] Secondary liquid expansion conditions: the fermentation temperature is 28°C, the oxygen flow rate can be 100-300L / h,

[0065] Initial pH: 7.0-7.5; rotating speed: 150-200r / min; tank pressure: 0.6-0.8Mpa, ferment and cultivate for 36 hours, and the spore concentration generally reaches 10 6 more than one / ml.

[0066] 3. Liquid fermentation culture:

...

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Abstract

The invention discloses a mixed plant protein fish feed as well as a preparation method and an application of the mixed plant protein fish feed. The mixed plant protein fish feed is prepared from the following raw materials by fermenting: a mixture of rapeseed meals and cotton meals, a bacillus subtilis fermenting solution, an enterococcus faecalis fermenting solution, an Aspergillus niger fermenting solution, a brewing yeast fermenting solution and an inorganic salt. The mixed plant protein fish feed is obtained by mixing and fermenting four bacteria including bacillus subtilis, enterococcusfaecalis, Aspergillus niger and a brewing yeast, so that an enzyme system is complete, the fermenting efficiency and the conversion are high, and the other-bacteria resisting capability is strong. The rapeseed meals and the cotton meals are mixed and are fermented and a material amino acid is more balanced; and the cost of raw materials is lower than that of the soybean meals. The yields of smallpeptides and free amino acids of the mixed plant protein fish feed can reach to 15.5% and 0.94%, respectively; and the removing rates of phytic acid, tannin and gossypol can reach to 87%, 78.2% and 100%, respectively. A fish feeding test result shows that after the mixed plant protein fish feed partially replaces fish powder or the rapeseed meals in the feed, the growth of fish can be obviously accelerated.

Description

technical field [0001] The present invention relates to fish feed, in particular to a mixed vegetable protein fish feed made by mixed fermentation of miscellaneous dregs and various microbial strains and a preparation method thereof. The present invention also relates to the application of the mixed vegetable protein fish feed as fish feed, which belongs to aquatic feed Raw material field. Background technique [0002] The global consumption of aquatic products is increasing year by year, and the dietary structure is changing. However, global marine fishing is limited, and the supply of aquatic products is mainly based on farmed aquatic products. China is the largest aquaculture country in the world. The total output of aquatic products accounts for 35% of the world, of which the output of aquaculture aquatic products accounts for about 70% of the world. While the consumer demand for aquatic products is growing rapidly, China's aquaculture water resources are showing a dow...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/14
Inventor 刘全礼蔡君曹芬谷序文凌华云
Owner WUHAN RICH GREEN AGRI BIOLOGICAL ENG TECH CO LTD
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