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Application of geraniin in preparing medicament for treating diseases caused by human enterovirus 71 type infection

A technology of human enterovirus and geranium, which can be applied in the field of medicine and can solve the problem of unreported inhibitory effect.

Active Publication Date: 2013-10-02
INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Geraniin has a variety of biological activities, including anti-oxidation, anti-bacterial, anti-tumor, anti-hypertensive, liver protection, inhibition of osteoclastogenesis and inhibition of a few viral replication, but its inhibitory effect on EV71 has not been reported so far

Method used

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  • Application of geraniin in preparing medicament for treating diseases caused by human enterovirus 71 type infection
  • Application of geraniin in preparing medicament for treating diseases caused by human enterovirus 71 type infection

Examples

Experimental program
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Embodiment 1

[0020] In the EV71-infected human rhabdomyoma cell model, the newly cultured 24-hour human rhabdomyoma cells were inoculated into 96-well plates, and the number of cells per well was 2×10 4 indivual. After 24 hours of cultivation in a 37°C incubator, the virus was inoculated.

[0021] The EV71 used is a popular strain in mainland China, the strain name is FY0805, isolated from Fuyang, Anhui, and the gene sequence is stored in NCBI as HQ882182. The dose of virus inoculated cells was 100TCID 50 , with a volume of 98 microliters, and continue to culture in a 37°C incubator.

[0022] Geraniin was dissolved in saline and administered 2 hours after the virus infected the cells in a drug volume of 2 microliters. The final drug concentrations are 0.1 μg / ml, 0.5 μg / ml, 1 μg / ml, 2 μg / ml, 5 μg / ml, 10 μg / ml, 20 μg / ml, 50 μg / ml, 100 μg / ml, 200 micrograms / ml. Observe the cytopathy within 3 days, and calculate the effective concentration of the drug according to the number of cells with...

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Abstract

The invention relates to the field of medicaments and particularly to an application of geraniin in preparing medicament for treating diseases caused by human enterovirus 71 type infection. According to the invention, EV71 infected people rhabdomyoma cell (RD) model and a mouse model established in a lab are utilized for a research on the geraniin which suppresses copying of human enterovirus 71 type and the effects of the geraniin on treating the diseases caused by human enterovirus 71 type infection. The research proves that the geraniin has good treatment effects and has the potential to become clinical therapeutic drugs.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of geraniin in the preparation of medicines for treating diseases caused by human enterovirus 71 infection. Background technique [0002] Enterovirus 71 (English name: Human enterovirus 71), referred to as EV71, is one of the main pathogens that cause hand-foot and mouth disease (HFMD) in infants. Human enterovirus 71 was first isolated from stool samples of infants with central nervous system diseases in California in 1969. These viruses can be cultured in rhesus monkey kidney cells (RhMK) and human embryos. Somatic cells (human fetal diploid cell). If the sample is taken from the patient's stool and tissue, it will be cultured with diploid strain of human fetal kidney cell (HFDK); if it is a throat swab sample, human embryonic lung diploid cell (diploid strain of human fetal lung cell) culture. After these cells were cultured, the purified virus was analyzed, and it w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P31/12
Inventor 张连峰杨亚军刘江宁
Owner INST OF LAB ANIMAL SCI CHINESE ACAD OF MEDICAL SCI