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Streptococcus alactolyticus and application thereof

A Streptococcus, Lactose Technology

Active Publication Date: 2012-09-19
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional water extraction and alcohol precipitation method is the most commonly used extraction method at present, and the extraction rate of astragalus polysaccharide is about 5.34% on average; the ultrafine pulverization method, the extraction rate of astragalus polysaccharide in the superfine powder is about 4.69%, and the extraction rate of astragalus polysaccharide in the coarse powder is about 4.69%. The extraction rate is only about 2.16%; the extraction rate of astragalus polysaccharide by ultrasonic cell crushing method is about 7.6%; the extraction rate of astragalus polysaccharide by microwave extraction method is about 6.55%; the extraction rate of astragalus polysaccharide by ultrafiltration is lower than that by water extraction and alcohol precipitation method , but the polysaccharide content in the product is high; the extraction rate of astragalus polysaccharide obtained by alkaline water extraction is between 4.7-19.2%, and the stability is poor; the extraction rate of astragalus polysaccharide obtained by alcohol-alkaline extraction can reach up to 19.15%; cellulase The extraction rate of astragalus polysaccharides by this method is higher than that by water extraction and alcohol precipitation method, and the content of polysaccharides in the extract is high, but there is no scientific reference data for the extraction rate due to unstable extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) the non-lactolytic streptococcus ( Streptococcus alactolyticus ) LZMYFGM9 CGMCC No.4227 strain (the inoculum size is 10 3 cfu) were cultured on a slope of MRS medium: time: 16 hours; environment: anaerobic fermentation; temperature: 37.5°C; initial pH: 6.4.

[0042] (2) The culture obtained in the previous step (the inoculum size is 10 3 cfu) into the primary or secondary seed medium for cultivation: time: 16 hours; environment: anaerobic fermentation; temperature: 37.5°C; initial pH: 5.9, and the fermentation inoculant was obtained after cultivation.

Embodiment 2

[0044] (1) the non-lactolytic streptococcus ( Streptococcus alactolyticus ) LZMYFGM9 CGMCC No.4227 strain (the inoculum size is 10 3 cfu) were cultured on a slope of MRS medium: time: 20 hours; environment: anaerobic fermentation; temperature: 36.5°C; initial pH: 6.0.

[0045] (2) The culture obtained in the previous step (the inoculum size is 10 3 cfu) into primary or secondary seed medium for cultivation: time: 20 hours; environment: anaerobic fermentation; temperature: 36.5°C; initial pH: 5.5, and fermented bacteria were obtained after cultivation.

Embodiment 3

[0047] (1) the non-lactolytic streptococcus ( Streptococcus alactolyticus ) LZMYFGM9 CGMCC No.4227 strain (the inoculum size is 10 3 cfu) were cultured on a slope of MRS medium: time: 18 hours; environment: anaerobic fermentation; temperature: 37°C; initial pH: 6.2.

[0048] (2) The culture obtained in the previous step (the inoculum size is 10 3 cfu) were inserted into primary or secondary seed medium for cultivation: time: 18 hours; environment: anaerobic fermentation; temperature: 37°C; initial pH: 5.7, and fermented bacteria were obtained after cultivation.

[0049] (3) Application examples

[0050] Non-lactolytic streptococcus LZMYFGM9 was used to extract astragalus polysaccharides in the fermentation reaction, specifically as follows:

[0051] (1) will prepare the containing non-lactolytic streptococcus ( Streptococcus alactolyticus ) The fermentation agent of LZMYFGM9 CGMCC No.4227 was transferred to the fermentation medium for extracting astragalus polysacchari...

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PUM

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Abstract

The invention discloses streptococcus alactolyticus LZMYFGM9 for the first time. The collection number of the strain is CGMCCNo.4227; and the invention also discloses a fermentation bacterium agent comprising the strain and an application thereof. The streptococcus alactolyticus in LZMYFGM9CGMCCNo4227 disclosed by the invention can be used for extracting astragalus polysaccharides. The method has the advantages of strong practicability, simple and convenient operation and high efficiency, and has a wide practical application prospect.

Description

technical field [0001] The present invention discovers a non-lactolytic streptococcus LZMYFGM9 for the first time, and the Latin classification of the strain is named as Streptococcus alactolyticus , the strain was deposited on October 19, 2010 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (abbreviation of the depository unit: CGMCC, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing), and the preservation number is CGMCC No. 4227. [0002] The invention relates to the field of fermentation, in particular to a non-lactolytic streptococcus ( Streptococcus alactolyticus ) LZMYFGM9 CGMCC No.4227 and its application. Background technique [0003] There are still few related studies on non-lactolytic streptococci, and the "Bergey's Bacterial Identification Manual" did not include non-lactolytic streptococci, which were first discovered by Farraw et al. in 1984. In 1984, Farrow et al. used the genetic class...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C08B37/00C12R1/46
Inventor 张凯李建喜张景艳孟嘉仁杨志强王学智
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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