Method for preparing red elemental selenium by utilizing super-selenium resistance microorganism

A technology of elemental selenium and microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of enhanced bacterial inhibition and achieve the effect of broad application prospects

Inactive Publication Date: 2012-10-03
于建泉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Li Ruiping et al. (2005) reported that Bacillus bacillus HBS4 has high biological tolerance to sodium selenite, and can still grow when the concentration of sodium selenite is as high as 11mM, but with the increase of selenium concentration, the inhibitory effect on bacteria is enhanced.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described in detail below in conjunction with the examples, but the embodiments of the invention are not limited thereto.

[0025] Selenium slag was collected from the Yutangba selenium deposit in Enshi, 3 grams were weighed, put into broth (commercially available finished product), and cultured at 35 °C for 6 hours.

[0026] Weigh 3.2 grams of nutrient agar medium (commercially available finished product), add 100 ml of distilled water to a 250ml Erlenmeyer flask, heat to dissolve, cover with a sponge stopper, sterilize under conventional high pressure, and divide into 5 sterile culture dishes to make a solid medium .

[0027] Pick up the enrichment solution with an inoculation loop and inoculate it on the nutrient agar medium by streaking method, culture at 35 °C for 24 hours, and isolate a single colony.

[0028] Carry out Gram staining, observe under the microscope of 1000 times, by Gram staining positive bacillus, have spore, ...

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Abstract

The invention relates to a method for preparing red elemental selenium by utilizing a super-selenium resistance microorganism. The preparation method is characterized by comprising the following steps of: a, breeding a target bacterial strain; b, preparing a selenium-contained culture medium; c, inoculating and culturing; d, preparing the red elemental selenium; e, producing the red elemental selenium; and f, harvesting the product. According to the method for preparing the red elemental selenium by utilizing the super-selenium resistance microorganism, the super-selenium resistance microorganism capable of normally surviving in a high-selenium environment is adopted, wherein the super-selenium resistance microorganism has the characteristic of reducing inorganic selenium into the red elemental selenium, can be used for preparing low-toxicity nanometer organic selenium and is wide in application prospect.

Description

technical field [0001] The invention relates to a method for preparing red elemental selenium by utilizing super selenium-resistant microorganisms. Background technique [0002] Microorganisms that have been reported to tolerate sodium selenite mainly include: Escherichia coli, Ralstonia CH34, Rhodospirillum rubrum, Capsulated Rhodobacter, Pseudomonas fluorescens, nitrogen-fixing Rhodobacter, yeast, etc. . However, the concentration of selenium tolerance is not high. For example, the concentration of sodium selenite that can be tolerated by Ralstonia CH43 is 6mmol / L, and the concentration of Escherichia coli that can tolerate sodium selenite is up to 20mmol / L. Wang Dongliang et al. (2007) reported a photosynthetic bacterium S3 that has high tolerance to sodium selenite and can reduce sodium selenite to red elemental selenium. Above this concentration no growth will occur. Yeast has a relatively high ability to enrich and transform selenium, usually about 300 μg / g. Through...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/07
Inventor 彭祚全黄剑锋
Owner 于建泉
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