Preparation of panaxadiol saponins component and pharmaceutical application for prevention and treatment of Parkinson disease

A technology of ginseng diol saponins and Parkinson's disease, applied in the field of medicine, can solve problems such as difficult medicinal value, involuntary movement, abnormality, etc., and achieve the effect of maintaining or restoring self-care ability and quality of life

Active Publication Date: 2012-10-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, dopa drugs have two major defects that cannot be overcome by themselves: one is the occurrence of motorfluctuation and abnormal, involuntary movements (abnormal involuntary movements) during treatment, which are defined as motor difficulties caused by levodopa ( Dyskinesia) or dyskinesia; with the prolongation of treatment time, the incidence rate continues to increase, about 50% after 5 years of treatment,

Method used

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  • Preparation of panaxadiol saponins component and pharmaceutical application for prevention and treatment of Parkinson disease
  • Preparation of panaxadiol saponins component and pharmaceutical application for prevention and treatment of Parkinson disease
  • Preparation of panaxadiol saponins component and pharmaceutical application for prevention and treatment of Parkinson disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Preparation of ginsengdiol saponin component-A (PDSF-A) from ginseng rhizome medicinal materials

[0059] 1. Separation and purification of ginsengdiol saponins component-A (PDSF-A) :

[0060] Ginseng rhizome medicinal material (8000g, sample-A, the five ginsengdiol saponins Rb contained in it 1 , Rc, Rb 2 , Rb 3 The content of Rd and Rd and their total content are shown in Table 5) After being crushed into coarse powder, the extract was percolated and extracted with 50% ethanol aqueous solution until the extract was colorless. The percolate was combined to recover the ethanol under reduced pressure and freeze-dried to obtain the extract (678.6 G). The extract was dissolved in 3000 ml of 45% ethanol aqueous solution, and the sample solution was separated by macroporous adsorption resin (Diaion HP-20, 6000 g) column chromatography. Firstly, it was eluted with 45% ethanol aqueous solution until the eluent had no obvious saponin The reaction was eluted with 70% et...

Embodiment 2

[0083] Example 2 Preparation of ginsenoside component-B (PDSF-B) from American ginseng rhizome medicinal materials

[0084] Use American ginseng root and stem medicinal materials (7000 grams, sample-B, which contains five ginsengdiol saponins Rb 1 , Rc, Rb 2 , Rb 3 The content of and Rd and their total content are shown in Table 5, and the HPLC spectrum is shown in Figure 1-3 ), using the same method as in Example 1 to prepare the ginsenoside component - B (PDSF-B, 101.3 g). HPLC analysis shows: PDSF-B also contains ginsenoside Rb 1 , Rc, Rb 2 , Rb 3 And Rd five kinds of ginsenosides, the total content of these five kinds of saponins is 91.60%, of which ginsenoside Rb 1 The highest content, followed by Rb 3 , Rd, Rb 2 And Rc; and Rb in ginsengdiol saponin component-B 1 / Rc / Rb 2 / Rb 3 / Rd content ratio and Rb in American ginseng root and stem medicinal materials (Sample-B) 1 / Rc / Rb 2 / Rb 3 The ratio of / Rd content is basically the same (Table 5).

Embodiment 3

[0085] Example 3 Preparation of ginsengdiol saponins component -C ( PDSF -C)

[0086] Purchased commercial ginseng rhizome total saponins (sample-C 1 , Its total saponins content ≥80%) and American ginseng stems and leaves total saponins (sample-C 2 , Its total saponins content ≥80%) HPLC analysis shows that the five ginsengdiol saponins contained in these two ginseng total saponins products have their own characteristics, namely: Sample-C 1 Both contain ginsenoside Rb 1 , Rc, Rb 2 , Rb 3 And Rd, but Rb 3 The content is very low (see attached diagram 2-1 And Table 5); Sample-C 2 Mainly containing Rb 3 And Rd, and Rb 1 , Rc and Rb 2 The content is very low (see attached Figure 2-2 And Table 5). This implementation comprehensively utilizes these two samples (Sample-C 1 And sample-C 2 ) Is used as the raw material to prepare the active component of ginsengdiol saponins of the present invention, especially the total content of five ginsengdiol saponins is ≥90%, and the content of th...

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PUM

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Abstract

The invention provides an application of a panaxadiol saponins component in the preparation of the drug for treating the Parkinson disease. The main components of the panaxadiol saponins component comprise Rb1, Rb2, Rb3, Rc and Rd. The drug panaxadiol saponins component is an active ingredient placed alone or together with other drugs and used for constituting the drug together with the pharmaceutically acceptable carriers. The drug is prepared by using the roots of Panax, the root of Panax quinquefolium, the stem leaf of Panax, the stem leaf of Panax quinquefolium and the total extract thereof or total saponins and other raw materials, and then performing chromatographic separation through the combined macroporous adsorption resin and octadecyl silane bonded silica gel column chromatography, purifying and the like. The panaxadiol saponins component provided by the invention can improve the motor function of PD patients, supplement the balance among a variety of neurological functions of the brain, fight against the dopaminergic neurons DEGradation of nigra-striatum system and the neurotoxicity of levodopa drugs, treat the neuropsychic disturbance of the PD patients, and maintain the validity of levodopa drugs in a long time. The structural formula of panaxadiol saponins is as shown in the specification.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the preparation of a panaxadiol saponins active component (hereinafter referred to as panaxadiol saponins fraction, Panaxadiol Saponins Fraction, PDSF) and a kind of panaxadiol saponins fraction (PDSF) in the preparation and prevention of Parkinson's Application in Syndrome Drugs. More specifically, the comprehensive utilization of ginseng, American ginseng medicinal materials and their stems and leaves and their extracts or total saponins to prepare ginseng diol saponin components; and ginseng diol saponin components alone or with other substances including known therapeutic Pa The pharmaceutical preparation made of Kimson's syndrome drugs, especially levodopa drugs, is used in the treatment and prevention of Parkinson's disease and the synergistic and attenuating effects of levodopa drugs. Background technique [0002] Parkinson's disease is a complex disease characterized by motor dysfu...

Claims

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Application Information

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IPC IPC(8): A61K31/704A61P25/16C07J17/00
Inventor 连晓媛张治针陈金龙
Owner ZHEJIANG UNIV
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